Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effects of Spider-Derived Protease (Arazyme®) Supplementation of Corn-Soy Diets on the Performance in Laying Hens at the Late Production

산란 후기 사료 내 거미(Nephila clavata) 유래 단백질 분해효소제(Arazyme®)의 첨가 급여가 난 생산성에 미치는 영향

  • Kim, J.U. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Kim, J.Y. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Kim, J.S. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Lee, B.K. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Lee, S.Y. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Lee, W.S. (College of Animal Bioscience and Technology, Konkuk University) ;
  • You, S.J. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Ahn, B.K. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Kim, E.J. (Division of Animal Care, Cheonan Yonam College) ;
  • Park, H.Y. (Korea Research Institute of Bioscience and Biotechnology) ;
  • Son, K.H. (Insect Biotech Co. Ltd.) ;
  • Shin, D.H. (Insect Biotech Co. Ltd.) ;
  • Kang, C.W. (College of Animal Bioscience and Technology, Konkuk University)
  • 김정언 (건국대학교 동물생명과학대학) ;
  • 김재영 (건국대학교 동물생명과학대학) ;
  • 김지숙 (건국대학교 동물생명과학대학) ;
  • 이보근 (건국대학교 동물생명과학대학) ;
  • 이소연 (건국대학교 동물생명과학대학) ;
  • 이완섭 (건국대학교 동물생명과학대학) ;
  • 유선종 (건국대학교 동물생명과학대학) ;
  • 안병기 (건국대학교 동물생명과학대학) ;
  • 김은집 (천안연암대학 동물복지계열) ;
  • 박호용 (한국생명공학연구원) ;
  • 손광희 ((주)인섹트바이오텍) ;
  • 신동하 ((주)인섹트바이오텍) ;
  • 강창원 (건국대학교 동물생명과학대학)
  • Published : 2009.03.31
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Abstract

This study was carried out to investigate the effects of spider-derived protease [Arazyme (AZ)] supplementation on egg production, egg quality, cecal microflora and viscosity of intestinal contents in laying hens at the late production. A total of two hundred, 59-wks-old, Hy-Line Brown layers were divided into four groups, placed into 5 replicates per group (50 birds per group) and fed one of four diets containing 0.05, 0.075 or 0.1% AZ or control for 5 wks. The results showed that egg weight in group fed the diet containing 0.05% AZ was significantly higher than those of other groups (P<0.05). There were no significant differences in egg interior and eggshell qualities among the groups. The serum parameters such as total cholesterol, the levels of GOT and GPT were not influenced by AZ feeding. There were no significant differences in the cecal number of total microbes, Coliforms and lactic acid bacteria among the groups. The viscosity and ammonia concentration of intestinal contents in the groups fed AZ were significantly reduced compared to those of the control (P<0.05). In conclusion, laying hens fed AZ supplemented diet improved egg weight as compared with that of control. In addition, the AZ supplemented diet reduced the viscosity and ammonia concentration of intestinal contents, reflecting a significant feed enzyme effect.

본 실험은 산란 후기 사료 내 거미 유래 단백질 분해효소 제 AZ의 첨가 급여가 산란계의 생산성, 계란의 품질, 혈액 특성, 장 내용물의 점도, 맹장 미생물 변화 및 맹장 내 암모니아 농도에 미치는 영향을 조사하기 위한 목적으로 수행하였다. 59주령의 Hy-Line Brown 실용계 200수를 공시하여 총 4개 처리에 각 처리구별 반복당 10수씩 5반복으로 완전 임의 배치하였고, 각각의 실험 사료에 AZ을 각각 0.05%, 0.075% 및 0.1% 혼합하여 총 5주간 급여하였다. 총 실험 기간 중 AZ 첨가에 따라 난중에서 유의한 차가 나타났으며, AZ 0.05%를 첨가한 처리구에서 가장 높은 증가가 나타났다(P<0.05). AZ 첨가는 난각 두께, 난각 강도, 난각색, 난황색 및 Haugh Unit는 처리구간 통계적 유의성은 없는 것으로 나타났다. 혈액 내 총 콜레스테롤 함량과 GOT 및 GPT 활성에 있어서는 처리구간 통계적 차이가 없는 것으로 나타났다. 맹장 내 미생물 균총을 조사해 본 결과 총 균수, 유산균수 및 대장균군수는 처리구간 차이를 보이지 않았다. 장 내용물의 점도 및 장내 암모니아 농도는 대조구에 비해 AZ을 첨가한 처리구에서 유의하게 감소하는 것으로 나타났다(P<0.05). 본 실험 결과, 식물성 및 동물성 단백질을 포함한 산란 후기 사료에 AZ의 첨가 급여는 난중 및 장 내 점도를 개선하고 암모니아 농도를 저감하는데 효과적이라는 것을 시사한다. 따라서 산란 후기 사료에 거미 유래 단백질 분해효소제 AZ의 첨가 급여는 산란 후기의 생산성, 장내 점도를 개선하여 사료 효소제로서 가치가 있는 것으로 판단된다. 또한 본 실험 결과 산란 후기 사료에서의 AZ의 첨가 수준은 0.05%와 0.075%에서 개선되는 결과가 나타났으나, 첨가 비용을 고려 할 때, 0.05% 첨가 수준이 가장 높은 효과가 있는 것으로 판단된다.

Keywords

References

  1. Bedford MR, Wyatt C 1998 Using feed enzymes to maximise nutrient utilisation in corn-soy or sorghum-soy based layer diets. Zootecnica Int 9:32-35
  2. Duncan DB 1955 Multiple range and multiple F test. Biometr 11:1-42 https://doi.org/10.2307/3001478
  3. Gao F, Jiang Y, Zhou GH, Han ZK 2007 The effects of xylanase supplementation on growth, digestion, circulatting hormone and metabolite levels, immunity and gut microflora in cockerels fed on wheat-based diets. Br Poult Sci 48:480-488 https://doi.org/10.1080/00071660701477320
  4. Gonzalez NJ, Cornejo S, Jimenez F, Lorenzoni G 2001 Effect of dietary supplementation with an enzyme complex (Avizyme$^{\circledR}$ 1500) containing amylase, xylanase and protease on performance and egg quality of commercial layer. Poult Sci 80:1040 (Abstr)
  5. Jaroni D, Scheideler SE, Beck MM, Wyatt C 1999a The effect of dietary wheat middlings and enzyme supplementation. 1. Late egg production efficiency, egg yields, and egg composition in two strains of Leghorn hens. Poult Sci 78:841-847 https://doi.org/10.1093/ps/78.6.841
  6. Jaroni D, Scheideler, Beck MM, Wyatt C 1999b The effect of dietary wheat middlings and enzyme supplementation. 2. Apparent nutrient digestibility, digestive tract size, gut viscosity, and gut morphology in two strains of Leghorn hens. Poult Sci 78:1664-1674 https://doi.org/10.1093/ps/78.12.1664
  7. Kwak JY, Lee KE, Shin DH, Maeng JS, Park DS, Oh HW, Son KH, Bae KS, Park HY 2007 Biochemical and genetic characterization of Arazyme, an extracellular metalloprotease produced from Serratia proteamaculans HY-3. J Microbiol Biotechnol 17:761-768
  8. Lumeij JT 1997 Avian clinical biochemistry. Pages 857-883 In: Clinical Biochemistry of Domestic Animals (eds. Kanebo, J. J., J. W. Harvey, and M. L. Bruss, 5th), Academic Press
  9. Mathlouthi N, Mohamed MA, Larbier M 2003 Effect of enzyme preparation containing xylanase and $\beta$-glucanase on performance of laying hens fed wheat/barley or maize/soybean meal based diets. Br Poul Sci 44:60-67 https://doi.org/10.1080/0007166031000085374
  10. Mourao JL, Ponte PIP, Prates JAM, Centeno MSJ, Ferreira LMA, Soares MAC, Fontes CMGA 2006 Use of $\beta$-glucanases and $\beta$-1,4-xylanase to supplement diets containing alfalfa and rye for laying hens: Effects on bird performance and egg quality. J Appl Poult Res 15:256-265 https://doi.org/10.1093/japr/15.2.256
  11. Nagaraj M, Hess JB, Bilgili SF 2007 Evaluation of a feed-grade enzyme in broiler diets to reduce pododermatitis. J Appl Poult Sci 16:52-61 https://doi.org/10.1093/japr/16.1.52
  12. NRC 1994 Nutrient Requirements of Poultry. 9th ed. National Academy Press. Washington D.C.
  13. Owens B, Tucker L, Collins MA, McCracken KJ 2008 Effects of different feed additives alone or in combination on broiler performance, gut microflora and ileal histology. Br Poult Sci 49:202-212 https://doi.org/10.1080/00071660802004890
  14. Roberts JR, Choct M 2006 Effects of commercial enzyme preparations on egg and eggshell quality in laying hens. Br Poult Sci 47:501-510 https://doi.org/10.1080/00071660600834175
  15. SAS 2002 SAS User's guide. Statistics, Version 8. e., SAS Institute. Inc. Cary, N.C.
  16. Scheidler SE, Beck MM, Abudabos A, Wyatt CL 2005 Multiple-Enzyme(Avizyme) supplementation of corn-soy-based layer diets. J Appl Poult Res 14:77-86 https://doi.org/10.1093/japr/14.1.77
  17. Short F, Hruby M, Burrows H, Bedford M 2002 The effect of a xylanase and protease enzyme on egg production in laying birds fed wheat based diets. Poult Sci 81(Suppl. 1):136
  18. Sohail SS, Bryant MM, Roland DA, Sr, Apajalahti JHA, Pierson EEM 2003 Infuluence of avizyme 1500 on performance of commercial Leghorns. J Appl Poult Res 12:284-290 https://doi.org/10.1093/japr/12.3.284
  19. Swiatkiewicz S, Koreleski J 2007 Effect of dietary level of maize-and rye distiller dried grains with solubles on nutrient utilization and digesta viscosity in laying hens. J Anim Feed Sci 16:668-677 https://doi.org/10.22358/jafs/66824/2007
  20. Wu G, Bryant MM, Voitle RA, Roland DA, Sr 2005 Effects of $\beta$-mannanase in corn-soy diets on commercial Leghorns in second-cycle hen. Poult Sci 84:894-897 https://doi.org/10.1093/ps/84.6.894
  21. Yoruk MA, Gol M, Hayirli A, Karaoglu M 2006 Multi-Enzyme supplementation to peak producing hens fed corn-soybean meal based diets. Int J Poult Sci 5:374-380 https://doi.org/10.3923/ijps.2006.374.380
  22. 김해진 조진호 진영걸 유종상 민병준 김인호 2007 갯벌 미생물 유래 단백질 분해 효소제의 급여가 산란계의 산란 생산성, 영양소 소화율 및 혈청내 총 단백질 함량에 미치는 영향. 한국가금학회지 34:9-14 https://doi.org/10.5536/KJPS.2007.34.1.009
  23. 민병준 김인호 홍종욱 문태현 이지훈 한영근 권오석 이상환 이원백 2002 산란계에서 탄수화물 분해효소제의 첨가가 계란의 품질 및 영양소 소화율에 미치는 영향. 한국가금학회지 29:19-23
  24. 이기은 김철희 권현정 곽장열 신동하 박두상 배경숙 박호용 2004 무당거미에서 분리한 Serratia proteamaculans에서 분비되는 단백질분해효소의 생화학적 특성. 한국미생물학회지 40:269-274
  25. 전흥규 2006 사료 내 미생물 Phytase 첨가가 닭과 돼지의 생산성과 영양소 이용성에 미치는 영향. 건국대학교 박사학위 청구논문