Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Establishing Test Method of Sporicidal activity of Commercial Sterilants

아포살균용 살균소독제 유효성 평가방법 확립

  • Kim, Hyung-Il (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation) ;
  • Jeon, Dae-Hoon (Food Additive Standard Division, Korea Food and Drug Administration) ;
  • Yoon, Hae-Jung (Food Chemical Residues Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kwak, In-Shin (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation) ;
  • Eom, Mi-Ok (Food Additive Standard Division, Korea Food and Drug Administration) ;
  • Sung, Jun-Hyun (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation) ;
  • Park, Na-Young (Food Additive Standard Division, Korea Food and Drug Administration) ;
  • Won, Sun-Ah (Food Additive Standard Division, Korea Food and Drug Administration) ;
  • Bae, Seo-Young (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation) ;
  • Lee, Young-Ja (Food Additive Standard Division, Korea Food and Drug Administration) ;
  • Kim, So-Hee (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation)
  • 김형일 (식품의약품안전평가원 첨가물포장과) ;
  • 전대훈 (식품의약품안전청 첨가물기준과) ;
  • 윤혜정 (식품의약품안전평가원 화학물질과) ;
  • 곽인신 (식품의약품안전평가원 첨가물포장과) ;
  • 엄미옥 (식품의약품안전청 첨가물기준과) ;
  • 성준현 (식품의약품안전평가원 첨가물포장과) ;
  • 박나영 (식품의약품안전청 첨가물기준과) ;
  • 원선아 (식품의약품안전청 첨가물기준과) ;
  • 배서영 (식품의약품안전평가원 첨가물포장과) ;
  • 이영자 (식품의약품안전청 첨가물기준과) ;
  • 김소희 (식품의약품안전평가원 첨가물포장과)
  • Published : 2009.12.31
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Abstract

Usually, bacterial spores are hundreds or thousands of times more resistant to chemical sanitizers than are vegetable bacteria. Consequently, it is hard to assess whether a commercial sterilant, containing hydrogen peroxide and peracetic acid as ingredients, has or does not have sporicidal activity under certain conditions using the National Standard Test Method for assessing bactericidal activity against Escherichia coli and Staphylococcus aureus. Hence we established alternative the standard test method and requirements to determine whether they are effective in showing at least reduction of $10^3$ in the number of Bacillus subtilis ATCC 6633 spores under the required test condition for evaluation of sporicidal activity including verification methodology. This standardized method has proved to be suitable for evaluating effectiveness of commercial sterilants and could be used as Standardization Test Method for assessing sporicidal activity.

세균포자는 살균소독제에 대해 영양세포보다 수백~수천배이상 저항성을 가지고 있기 때문에 과산화수소 및 과산화초산을 원료로 하는 아포살균용 살균소독제는 Escherichia coli 및 Staphylococcus aureus의 감소율을 측정하는 현행 살균소독제 유효성 평가방법을 적용하기 어렵다. 따라서, 아포살균용 살균속독제의 유효성 평가를 위해 Bacillus subtilis ATCC 6633 포자를 표준균주로 하여 아포살균용 살균소독제를 처리했을 때 초기포자수를 $10^3$이상 감소시키는지를 측정하는 정량적 표준시험방법을 확립하였다. 확립된 시험법은 아포살균력을 측정하는 국가표준시험법으로서 아포살균용 살균소독제의 유효성 평가에 적합하였다.

Keywords

References

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