Journal of Microbiology and Biotechnology

  • 제19권12호
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  • Pages.1688-1694
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  • 2009
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Characterization of a Phenazine and Hexanoyl Homoserine Lactone Producing Pseudomonas aurantiaca Strain PB-St2, Isolated from Sugarcane Stem

  • Mehnaz, Samina (School of Biological Sciences, Quaid-e-Azam Campus, University of the Punjab) ;
  • Baig, Deeba Noreen (School of Biological Sciences, Quaid-e-Azam Campus, University of the Punjab) ;
  • Jamil, Farrukh (School of Biological Sciences, Quaid-e-Azam Campus, University of the Punjab) ;
  • Weselowski, Brian (Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada) ;
  • Lazarovits, George (Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada)
  • 발행 : 2009.12.31
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초록

A novel strain of fluorescent pseudomonad (PB-St2) was isolated from surface-sterilized stems of sugarcane grown in Pakistan. The bacterium was identified as Pseudomonas aurantiaca on the basis of 16S rRNA gene sequence analysis and results from physiological and biochemical characteristics carried out with API50 CH and QTS 24 bacterial identification kits. Assays using substrate-specific media for enzymes revealed lipase and protease activities but cellulase, chitinase, or pectinase were not detected. The bacterium was unable to solubilize phosphate or produce indole acetic acid. However, it did produce HCN, siderophores, and homoserine lactones. In dual culture assays on agar, the bacterium showed antifungal activity against an important pathogen of sugarcane in Pakistan, namely Colletotrichum falcatum, as well as for pathogenic isolates of Fusarium oxysporium and F. lateritium but not against F. solani. The antifungal metabolites were identified using thin-layer chromatography, UV spectra, and MALDI-TOFF spectra and shown to be phenazine-1-carboxylic acid (PCA), 2-hydroxyphenazine (2-OH-PHZ), and N-hexanoyl homoserine lactone (HHL) (assessed using only TLC data). The capacity of this bacterium to produce HCN and 2-OH-PHZ, as well as to inhibit the growth of C. falcatum, has not been previously reported.

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  2. Enhanced production of 2-hydroxyphenazine in Pseudomonas chlororaphis GP72 vol.89, pp.1, 2009, https://doi.org/10.1007/s00253-010-2863-1
  3. Elucidation of Antifungal Metabolites Produced by Pseudomonas aurantiaca IB5-10 with Broad-Spectrum Antifungal Activity vol.22, pp.3, 2009, https://doi.org/10.4014/jmb.1106.06042
  4. Evaluation ofPseudomonas chlororaphissubsp.aurantiacaSR1 for growth promotion of soybean and for control ofMacrophomina phaseolina vol.24, pp.9, 2014, https://doi.org/10.1080/09583157.2014.910293
  5. Complete Genome Sequence of the Sugar Cane Endophyte Pseudomonas aurantiaca PB-St2, a Disease-Suppressive Bacterium with Antifungal Activity toward the Plant Pathogen Colletotrichum falcatum vol.2, pp.1, 2009, https://doi.org/10.1128/genomea.01108-13
  6. Reaction Kinetics for the Biocatalytic Conversion of Phenazine-1-Carboxylic Acid to 2-Hydroxyphenazine vol.9, pp.6, 2009, https://doi.org/10.1371/journal.pone.0098537
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  8. Genetic engineering of Pseudomonas chlororaphis GP72 for the enhanced production of 2-Hydroxyphenazine vol.15, pp.None, 2009, https://doi.org/10.1186/s12934-016-0529-0
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  11. Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass vol.27, pp.3, 2017, https://doi.org/10.4014/jmb.1601.01021
  12. Metabolic and Genomic Traits of Phytobeneficial Phenazine-Producing Pseudomonas spp. Are Linked to Rhizosphere Colonization in Arabidopsis thaliana and Solanum tuberosum vol.86, pp.4, 2009, https://doi.org/10.1128/aem.02443-19
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