Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
권호 표지
DOI QR코드

DOI QR Code

NMR Studies of Ni-binding Luteinizing Hormone Releasing Hormone

  • Kim, Jin (Department of Applied Chemistry, Hanyang University) ;
  • Won, Ho-Shik (Department of Applied Chemistry, Hanyang University)
  • Published : 2009.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

Luteinizing Hormone Releasing Hormone (LHRH) is composed of 10 amino acids, and is best known as a neurotransmitter. Because of the 80% homology in animals, much more concerns have focused on the substances that have similar functions or can control LHRH. Ni, Cu-LHRH complexes were synthesized. The degree of complexation was monitored by $^1H,\;^{13}C$-NMR chemical shifts, and final products were identified by ESI-Mass spectrum. Solution-state structure determination of Ni-LHRH complex was accomplished by using NMR results and NMR-based distance geometry (DG). Interproton distances from nuclear Overhauser effect spectroscopy (NOESY) were utilized for the molecular structure determination. Results were compared with previous structures obtained from energy minimization and other spectroscopic methods. Structure obtained in this study has a cyclic conformation which is similar to that of energy minimized, and exhibits a specific a-helical turn with residue numbers (2~7) out of 10 amino acids. Comparison of chemical shifts and EPR studies of Ni, Cu-LHRH complexes exhibit that Ni-LHRH complex has same binding sites with the 4-coordination mode as in Zn-LHRH complex.

Keywords

References

  1. Matsuo, H., Baba, Y., Nair, R. M. G., Schally, A. V., Biochem. Biophys. Res. Commun. 43, 1334-1339 (1971) https://doi.org/10.1016/S0006-291X(71)80019-0
  2. Baba, Y., Matsuo, H., Schally, A. V., Biochem. Biophys. Res. Commun., 44, 459-463 (1971) https://doi.org/10.1016/0006-291X(71)90623-1
  3. Schally, A. V., Arimura, A., Carter, W. H., Redding, T. W., Geiger, R., Kraig, W., Wissman, H., Jaeger, G., Sandow, J., Biochem. Biophys. Res. Commun., 48, 366-375 (1972) https://doi.org/10.1016/S0006-291X(72)80060-3
  4. Hehrman, H. R., Aten, R. F., Marcolin, Y., Can. J. Physiol. Pharmacol., 67, 954-956 (1989) https://doi.org/10.1139/y89-150
  5. Hsueh, A. J., Jones, P. B. C., Endocrine. Reviews, 2, 437-461 (1981) https://doi.org/10.1210/edrv-2-4-437
  6. Smith, R. A., Branca, A. A., Reichert Jr., L. E., J. Biol. Chem., 260, 14297-14303 (1985)
  7. Ascoli, M., Segaloff, D. L., Endocrin. Reviews., 11, 27-44 (1989)
  8. Shin, J., Ji, T. H., J. Biol. Chem., 260, 12828-12831 (1985)
  9. van Ginkel, K. A., Loeber, J. G., Acta Endocrinologica, 121, 73-82 (1989)
  10. Hooper, N. M., Kenny, A. J., Turner, A. J., Biochem. J., 231, 357-361 (1985) https://doi.org/10.1042/bj2310357
  11. Hooper, N. M., Turner, A. J., Bichem. J., 241, 625-633 (1987)
  12. Turner, A. J., Neuropeptides and Their Peptidases,,165-198, VCH, New York (1987)
  13. Carnone, F. A., Stetler-Stevenson, M. A., May, V., Labarbera, A., Flouret, G., Am. J. Physiol., 253, E317-E321 (1987)
  14. Coy, D. H., Coy, E. J., Schally, A. V., Vilchez-Martinez, J., Hirotsu, Y., Arimura, A., Biochem. Biophys. Res. Commun., 57, 335-340 (1974) https://doi.org/10.1016/0006-291X(74)90934-6
  15. Monoahan, M. W., Amoss, M. S., Anderson, H. A., Vale, W., Biochemistry, 12, 4616-4620 (1973) https://doi.org/10.1021/bi00747a012
  16. Coy, D. H., Coy, E. J., Schally, A. V., J Med. Chem., 16, 1140-1143 (1973) https://doi.org/10.1021/jm00268a016
  17. Kitajima, Y., Catt, K. J., Chen, H., Biochem. Biophys. Res. Comm., 159, 893-898 (1989) https://doi.org/10.1016/0006-291X(89)92192-X
  18. Paul, P. K. C., Dauber-Osguthrope, P., Campbell, M. M., Osguthrope, D. J., Biochem. Biophys. Res. Comm., 165, 1051-1058 (1989) https://doi.org/10.1016/0006-291X(89)92708-3
  19. Dutta, A. S., Gormley, J. J., McLaclan, P. F., Woodburn, J. R., Biochem. Biophys. Res. Comm, 159, 1114-1120 (1989) https://doi.org/10.1016/0006-291X(89)92224-9
  20. Momany, F. A., J. Med. Chem., 21, 63-68 (1978) https://doi.org/10.1021/jm00199a011
  21. Burrows, G. H., Barnea, A., Endocrinlogy, 110, 1456-1458 (1982) https://doi.org/10.1210/endo-110-4-1456
  22. Won, H.; Olson, K. D.; Wolfe, R. S.; Summers, M. F. J. Am. Chem. Soc., 112, 2178-2184 (1990) https://doi.org/10.1021/ja00162a020
  23. Won, H.; Olson, K. D.; Hare, D. R.; Wolfe, R. S.; Kratky, C.; Summers, M. F. J. Am. Chem. Soc., 114, 6880-6887 (1992) https://doi.org/10.1021/ja00043a038
  24. D. Kim, J. Rho, H. Won, J. Kor. Mag. Reson. Soc., 3, 44-51 (1999)