Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Dysregulation of Cannabinoid CB1 Receptor Expression in Subcutaneous Adipocytes of Obese Individuals

  • Lee, Yong-Ho (Diabetes and Metabolism Translational Medicine Unit, University of Vermont College of Medicine) ;
  • Tharp, William G. (Diabetes and Metabolism Translational Medicine Unit, University of Vermont College of Medicine) ;
  • Dixon, Anne E. (Division of Pulmonary and Critical Care Medicine, University of Vermont College of Medicine) ;
  • Spaulding, Laurie (Department of Surgery, University of Vermont College of Medicine) ;
  • Trost, Susanne (Diabetes and Metabolism Translational Medicine Unit, University of Vermont College of Medicine) ;
  • Nair, Saraswathy (Center for Biomedical Studies, University of Texas at Brownsville) ;
  • Permana, Paska A. (Phoenix Veterans Affairs Health Care System) ;
  • Pratley, Ridhard E. (Diabetes and Metabolism Translational Medicine Unit, University of Vermont College of Medicine)
  • Published : 2009.12.31
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Abstract

The endocannabinoid system (ECS) plays a key role in the regulation of appetite, body weight and metabolism. We undertook the present study to further clarify the regulation of the cannabinoid CB1 receptor (CB1, CNR1) in human adipose tissue in obesity. CB1 receptor mRNA expression was ~1.6-fold (p<0.004) and 1.9-fold higher (P<0.05) in subcutaneous adipocytes from obese compared to non-obese subjects in microarray and quantitative real-time PCR studies, respectively. Higher CB1 receptor mRNA expression levels in both adipose tissue (~1.2 fold, P<0.05) and adipocytes (~2 fold, P<0.01) were observed in samples from visceral compared to subcutaneous depots collected from 22 obese individuals. Immunofluorescence confocal microscopy demonstrated the presence of CB1 receptor on adipocytes and also adipose tissue macrophages. These data indicate that adipocyte CB1 receptor is up-regulated in human obesity and visceral adipose tissue and also suggest a potential role for the ECS in modulating immune/inflammation as well as fat metabolism in adipose tissue.

Keywords

References

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