Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Early Survival and Karyotype of Backcross Bullhead Hybrid between Pseudobagrus fulvidraco Female and P. fulvidraco $\times$ Leiocassis ussuriensis Hybrid Male

동자개 Pseudobagrus fulvidraco 암컷과 동자개 P. fulvidraco $\times$ 대농갱이 Leiocassis ussuriensis 잡종 수컷간 역교배체의 초기 생존을 및 염색체 핵형

  • Park, Sang-Yong (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Kang, Eun-Jong (Southern Regional Inland Fisheries Research Institute, NFRDI) ;
  • Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 박상용 (순천향대학교 해양생명공학과) ;
  • 강언종 (국립수산과학원 남부내수면연구소) ;
  • 남윤권 (부경대학교 양식학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Published : 2009.02.25
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Abstract

Backcross hybridization between Korean bullhead Pseudobagrus fulvidraco female and Korean bullhead P. fulvidraco $\times$ Ussurian bullhead Leiocassis ussuriensis hybrid male was performed, and early viability and karyotype of the backcross hybrids were examined along with their parental species. Mean fertilization rate (86.8%), hatching success (70.7%) and early survival rate (76.4%) of backcross hybrids were similar with those found in the maternal species, the Korean bullhead. From the chromosome analysis, modal chromosome numbers of Korean bullhead, Ussurian bullhead, their hybrid and backcross hybrid were the same as 2n = 52. However, their karyotypes were different among genotypes. The karyotype of backcross hybrid was 22 metacentric + 18 submetacentacentric + 12 acrocentric chromosomes.

동자개(PF) 암컷과 대농갱이(LU) 수컷에 의해 유도된 잡종(FU)을 대상으로 동자개 및 대농갱이 암컷과 역교배체(PFU, LFU)를 유도하여 수정률, 부화율, 초기생존을, 염색체 수 및 핵형분석을 수행하였다. 유도된 PFU ($PF{\times}FU$)의 수정률은 86.8%, 부화율은 70.7% 및 생존율은 76.4%로 양친으로 사용된 PF 및 LU보다 유의적으로 높게 나타났다. 그러나 FU를 사용한 LFU ($PF{\times}FU$)는 모두 사망하였다. PF (2n=52)와 FU(2n=52)간의 PFU의 염색체 수는 2n = 52로 양친과 동일하게 나타났으며 핵형분석 결과 22 metacentrics + 20 submetacentric + 10 acrocentric으로 구성되어 있었다.

Keywords

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