Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Immunoblot Patterns of Taenia asiatica Taeniasis

  • Jeon, Hyeong-Kyu (Department of Parasitology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Eom, Kee-Seon S. (Department of Parasitology and Medical Research Institute, Chungbuk National University College of Medicine)
  • Published : 2009.03.31
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Abstract

Differential diagnosis of Taenia asiatica infection from other human taeniases by serology has been tested. An enzyme-linked immunoelectrotransfer blot (EITB) was applied to subjected human sera and tapeworm materials. Thirty-eight proteins reactive to serum IgG were observed between 121 and 10 kDa in adult worms, and more than 22 serum-reactive components between 97kDa and 21.5kDa were observed in eggs of T. asiatica. Antigens of adult T. asiatica revealed immunoblot bands between 120 and 21.5 kDa against T. asiatica infected sera. Antigens of adult Taenia saginata revealed 110-100, 66, 58-56, and 46 kDa immunoblot bands against T. asiatica infected sera. Antigens of adult Taenia solium also revealed 99-97, 68-66, and 46 kDa bands against T. asiatica infected sera. The immunoblot band of 21.5 kDa exhibited specificity to T. asiatica.

Keywords

References

  1. Eom KS, Rim HJ. Morphologic description of Taenia asiatica sp.n. Korean J Parasitol 1993; 31: 1-6 https://doi.org/10.3347/kjp.1993.31.1.1
  2. Eom KS, Rim HJ. Natural infections of Asian Taenia saginata metacestodes in the livers of Korean domestic pigs. Korean J Parasitol 1992; 30: 15-20 https://doi.org/10.3347/kjp.1992.30.1.15
  3. Eom KS, Jeon HK, Kong Y, Hwang UW, Yang Y, Li X, Xu L, Feng Z, Pawlowski ZS, Rim HJ. Identification of Taenia asiatica in China:molecular, morphological, and epidemiological analysis of a Luzhai isolate. J Parasitol 2002; 88: 758-764 https://doi.org/10.1645/0022-3395(2002)088[0758:IOTAIC]2.0.CO;2
  4. Allan JC, Avila G, Garcia-Noval J, Flisser A, Craig PS. Immunodiagnosis of taeniasis by corproantigen detection. Parasitology 1990; 101: 473-477 https://doi.org/10.1017/S0031182000060686
  5. Allan JC, Mencos F, Garcia-Noval J, Sarti E, Flisser A, Wang Y, Liu D, Craig PS. Dipstick dot ELISA for the detection of Taenia corproantigens in humans. Parasitology 1993; 107: 79-85 https://doi.org/10.1017/S0031182000079439
  6. Allan JC, Velasquez-Tohom M, Torres-Alvarez R, Yurrita P, Garcia-Noval J. Field trial of the corproantigen-based diagnosis of Taenia solium taeniasis by enzyme-linked immunosorbent assay. Am J Trop Med Hyg 1996; 54: 352-356
  7. Chapman A, Vallejo V, Mossie KG, Ortiz D, Agabian N, Flisser A. Isolation and characterization of species-specific DNA probes from Taenia solium and Taenia saginata and their use in an egg detection assay. J Clin Microbiol 1995; 33: 1283-1288
  8. Yamasaki H, Allan JC, Sato MO, Nakao M, Sako Y, Nakaya K, Qiu D, Mamuti W, Craig PS, Ito A. DNA differential diagnosis of taeniasis and cysticercosis by multiflex PCR. J Clin Microbiol 2004;42: 548-553 https://doi.org/10.1128/JCM.42.2.548-553.2004
  9. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976; 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  10. Tsang VCW, Peralta JM, Simons AR. Enzyme-linked immunotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Method Enzymol 1985; 92: 377-391 https://doi.org/10.1016/0076-6879(83)92032-3
  11. Simanjuntak GM, Margono SS, Okamoto M, Ito A. Taeniasis/cysticercosis in Indonesia as an emerging disease. Parasitol Today 1997; 13: 321-323 https://doi.org/10.1016/S0169-4758(97)01104-6
  12. Fan PC, Chung WC. Taenia saginata asiatica: epidemiology, infection, immunological and molecular studies. J Microbiol Immunol Infect 1998; 31: 84-89
  13. Eom KS, Rim HJ. Epidemiological understanding of Taenia tapeworms infections with special reference to Taenia asiatica in Korea. Korean J Parasitol 2001; 39: 267-283 https://doi.org/10.3347/kjp.2001.39.4.267
  14. Willingham AL, De NV, Doanh NQ, Cong le D, Dung TV, Dorny P, Cam PD, Dalsgaard A. Current status of cysticercosis in Vietnam. Southeast Asian J Trop Med Public Health 2003; 34(suppl 1):35-50
  15. Anantaphruti MT, Yamasaki H, Nakao M, Waikagul J, Watthanakulpanich D, Nuamtangong S, Maipanich S, Pubampen S, Sanguankiat S, Muennoo C, Nakaya K, Sato MO, Sako Y, Okamoto M, Ito A. Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand. Emerg Infec Dis 2007; 13: 1413-1416 https://doi.org/10.3201/eid1309.061148
  16. Ito A, Yamasaki H, Nakao M, Sako Y, Okamoto M, Sato MO, Nakaya K, Margono SS, Ikejima T, Kassuku AA, Afonso SM, Ortiz WB, Plancarte A, Zoli A, Geerts S, Craig PS. Multiple genotypes of Taenia solium-ramifications for diagnosis, treatment and control. Acta Trop 2003; 87: 95-101 https://doi.org/10.1016/S0001-706X(03)00024-X
  17. Fan PC, Chung WC, Lin CY, Wu CC. The pig as an intermediate host for Taiwan Taenia infection. J Helminthol 1990; 64: 223-231 https://doi.org/10.1017/S0022149X00012207
  18. Galan-Puchades MT, Fuentes MV. Human cysticercosis and larval tropism of Taenia asiatica. Parasitol Today 2000; 16: 174

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