DOI QR코드

DOI QR Code

HPLC를 이용한 인삼 진세노사이드의 최적 분석 조건 및 홍삼 제품과 원료삼의 진세노사이드 함량 분석

Optimal Analytical Conditions for Panax Ginseng Ginsenosides using HPLC and Ginsenosides Content Analysis of Red Ginseng Products and their Raw Materials

  • 탁근만 (호서대학교 식품생물공학과) ;
  • 손민희 (호서대학교 식품생물공학과) ;
  • 채희정 (호서대학교 식품생물공학과)
  • 발행 : 2009.02.28

초록

인삼사포닌인 진세노사이드의 분석조건을 고성능액체크로마토그래피(HPLC)를 이용하여 최적화하였다. Gradient 조건을 변경하여 최적 분석조건을 확립하였고, 같은 조건하에서 인삼원재료와 홍삼 제품 중의 진세노사이드를 분석하였다. 원재료 중에서는 홍삼이 Rg1, Rb1, Rc, Rb2, Rd를 각각 0.29%, 0.82%, 0.33%, 0.32%, 0.11%를 함유하여 가장 높은 진세노사이드 함량을 보였으며, 여러 가지 홍삼제품 중에서는 홍삼엑기스가 가장 높은 함량을 보였다. 인삼 원료와 제품의 종류마다 진세노사이드 함량에 차이를 보였지만 대부분의 원료와 제품에서 Re, Rb1이 그 중 에서도 가장 높은 함량을 보였다.

The analytical conditions of ginsenosides were optimized using high performance liquid chromatography (HPLC). The optimal analysis conditions were set up from the experiments using different gradient conditions, and the ginsenosides contents of red ginseng products and their raw materials were analysed. Red ginseng contained 0.29% Rg1, 0.82% Rb1, 0.38% Rc, 0.32% Rb2, 0.11% Rd, showing the highest ginsenoside contents. Among the red ginseng products, red ginseng extract showed the highest content. The ginsenoside contents were varied according to the raw material type and product type. Re and Rb1 contents were the highest in most raw materials and products.

키워드

참고문헌

  1. Watanabe, J., Oh, K. W., Kim, H. S., M. Tanahashi and H. Kaneto. (1988), J. Pharmacobio-Dyn, 11, 453-458. https://doi.org/10.1248/bpb1978.11.453
  2. Brekhman, I. I. (1957), Panax ginseng. Gosudarst Isdatet Med, 2, 10-24.
  3. Namba, T. (1980), The Encyclopedia of Wakan -Yaku with Color Pictures. Hoikusha, Osaka, Japan, 23-48.
  4. Shibata, S., Tanaka, O., Soma, K., lita, Y., Ando, T. and Nakamura H. (1965), Studies on saponins and sapogenins of ginseng: the structure of panaxatriol. Tetrahedron Lett, 3, 207-213.
  5. Shibata, S. (1974), Some chemical studies on ginseng, Proc. Intern. Ginseng Sym, 69-76.
  6. Park, J. D. (1996), Recent studies on the chemical constituents of Korean ginseng. Korean J. Ginseng Sci, 20, 389-415.
  7. Sanata, S., Kondo, N., Shoji, J., Tanaka, O. and Shibata, S. (1974), Studies on the saponins of ginseng. I Structure of ginseng-R0, Rb1, Rb2, Rc and Rd. Chem. Pharm. Bull, 22, 421-428. https://doi.org/10.1248/cpb.22.421
  8. Kitagawa, I., Taniyama, T., Shibuya, H., Nota, T. and Yoshikawa, M. (1987), Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2) ;Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root. Yakugaku Zasshi, 107, 495-505. https://doi.org/10.1248/yakushi1947.107.7_495
  9. Yokozawa, T., Kobayashi, T., aura, H. and Kawashima, Y. (1985), Studies on the mechanism of the hypoglycemic activity of ginsenoside-Rb2 in streptozotocin-diabetic rats. Chem. Pharm. Bull, 33, 869-872. https://doi.org/10.1248/cpb.33.869
  10. Keum, Y. S., Park, K. K., Lee, J. M., Chun, K. S., Park, J. H., Lee, S. K., Kwon, H. and Surh, Y. J. (2000), Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Lett, 150, 41-48. https://doi.org/10.1016/S0304-3835(99)00369-9
  11. Kim, S. E., Lee, Y. H., Park, J. H. and Lee, S. K. (1999), Ginsenoside-Rs3, a new diol-type ginseng saponin, selectively elevates protein levels of p53 and p21WAF1 leading to induction of apoptosis in SK-HEP-1 cells. Anticancer Res, 19, 487-491. https://doi.org/10.1097/CAD.0b013e3282fd5310
  12. Kim, W. Y., Kim, J. M., Han, S. B., Lee, S. K., Kim, N. D., Park, M. K., Kim, C. K. and Park, J. H. (2000), Steaming of ginseng at high temperature enhances biological activity. J. Nat. Prod, 63, 1702-1704 https://doi.org/10.1021/np990152b
  13. Bao, H. Y., Zhang, J., Yeo, S. J., Myung, C. S., Kim, H. M., Kim, J. M., Park, J. H., Cho, J. S. and Kang, J. S. (2005), Memory enhancing and neuroprotective effects of selected ginsenosides. Arch. Pharm. Res, 28, 335-342. https://doi.org/10.1007/BF02977802
  14. Jung, K. Y., Kim, D. S., Oh, S. R., Lee, I. S., Lee, J. J., Park, J. D., Kim, S. I. and Lee, H. K. (1998), Platelet activating factor antagonist activity of ginsenosides. Biol. Pharm. Bull, 21, 79-80. https://doi.org/10.1248/bpb.21.79
  15. Y. Saruwatari, H. Besso, K. Futamura, et al., (1979), Chem. Pharm. Bull, 27, 147 - 151. https://doi.org/10.1248/cpb.27.147
  16. H. oura, S. hiai, Y. odaka and T. yokozawa, (1975), Studies on the Biochemical Action of Ginseng Saponin. J. Biochem, 77, 1057-1065. https://doi.org/10.1093/oxfordjournals.jbchem.a130806
  17. Sticher, O. and Solidat,i F. (1979), HPLC separation and quantitative determination of ginseno- sides from Panax giseng, Panax quinquefolium and from ginseng drug preparations. Planta Med, 36, 30-42. https://doi.org/10.1055/s-0028-1097237
  18. Park, M. K., Park, J. H., Kang, J. S., Lee, M. Y., Park, Y. I., Yu, S. J. and Han, B. H. (1993), Rapid hydrolysis of ginseng saponin by microwave oven reaction. Kor. J. Ginseng Sci, 17, 35-38.
  19. Saeki, T., Koide, K. and Nikaide, T. (1999), A comparative study on commercial, botanical gardens and wild samples of the roots of Platycodon grandiflorum by HPLC analysis. Planta med. 65, 428-431. https://doi.org/10.1055/s-1999-14021
  20. Kim, G. S., Kim, H. T., Seong. J. D., Park, H. S. and Kim, S. D. (2002), Quantitative analysis of platycodin d from Platycodon grandiflorum by HPLC-ELSD. Korean J. Medicinal Crop Sci, 10, 200-205.
  21. Park, J. Y., Lee, C. Y., Won, J. Y. (2007). Analytical optimum of ginsenosides according to the gradient elution of mobile phase in high performance liquid chromatography. Kor. J. Medicinal Crop Sci, 15, 215-219.
  22. Matsuda, h., Samukawa. K. and Kubo. M. (1998), The 13th Symo. The Medical society for red ginseng research (Abstract), 8
  23. Zhang, J. T., Qu, Z. W., Liu, Y. and Deng, H. L. (1990). Chin. Med. J, 103, 932
  24. Zhang, J. T., Yang, Y., Qu, Z. W., Jing, X. Y. and Liu, M. (1993). Proc. of 6th Int'l Ginseng Symposium, Korea Ginseng & Tobacco Research Institute, Korea, 69
  25. Han, Y. M., Jin, B. S., Ko, S. K. and Lee, J. H. (2004), Immunoactivity of ginsenoside Re and Rg1 that enhances resistance of mice against experimental disseminated candidiasis. Natural Product Sci, 10, 134-139.
  26. Choi, K. J., Go, S. R., Kim. S. C. and Park, J. D. (1989), Saponin and ginsenoside content in Korea red ginseng products. Kor. J. Ginseng Sci, 13, 178-182.
  27. Ko, S. K., Lee, K. H., Hong, J. K., Kang, S. A,. Sohn, U. D., Im, B. O., Han, S. T., Yang, B. W., Chung, S. H. and Lee, B. Y. (2005), Change of ginsenoside composition in Ginseng extract by vinegar process. Food Sci. Biotechnol, 14, 509-513.