Mycobiology

  • 제37권1호
  • /
  • Pages.62-66
  • /
  • 2009
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

Occurrence of Internal Stipe Necrosis of Cultivated Mushrooms (Agaricus bisporus) Caused by Ewingella americana in Korea

  • Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jhune, Chang-Sung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cheong, Jong-Chun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yun, Hyung-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Weon-Dae (National Academy of Agricultural Science, RDA)
  • 발행 : 2009.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The internal stipe necrosis of cultivated mushrooms (Agaricus bisporus) is caused by the bacterium Ewingella americana, a species of the Enterobacteriaceae. Recently, Ewingella americana was isolated from cultivated white button mushrooms in Korea evidencing symptoms of internal stipe browning. Its symptoms are visible only at harvest, and appear as a variable browning reaction in the center of the stipes. From these lesions, we isolated one bacterial strain (designated CH4). Inoculation of the bacterial isolate into mushroom sporocarps yielded the characteristic browning symptoms that were distinguishable from those of the bacterial soft rot that is well known to mushroom growers. The results of Gram stain, flagellal staining, and biochemical tests identified these isolates as E. americana. This was verified by pathogenicity, physiological and biochemical characteristics, and the results of an analysis of the 16S rRNA gene sequences and the fatty acids profile. This is the first report of the isolation of E. americana from cultivated white button mushrooms in Korea.

키워드

참고문헌

  1. Braithwaite, M., Pay, J. M. and Eade, K. 2005. First record of Ewingella americana on cultivated mushrooms in New Zealand. Proc. and Abstr. of the 15th biennial Australasian plant pathology conference. p. 96. Australasian Plant Pathology Society, Geelong
  2. Cowan, S. T. and Steel, K. J. 1965. Manual for the Identification of Medical Bacteria. Cambridge University Press, London
  3. Dowson, W. J. 1957. Plant disease due to bacteria. Cambridge University Press, London
  4. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the booststrap. Evolution 39:783-791 https://doi.org/10.2307/2408678
  5. Grimont, P. A., Farmer, J. J., Grimont III, F., Asbury, M. A., Brenner, D. J. and Deval, C. 1983. Ewingella americana gen. nov., sp. nov., a new Enterobacteriaceae isolated from clinical specimens. Ann. Microbiol. (Paris) 134:39-52
  6. Inglis, P. W. 1995. Internal stipe necrosis of Agaricus bisporus-Etiology and Molecular Genetic Studies. Ph.D. Thesis, University of Nottingham, University Park, Nottingham, UK
  7. Inglis, P. W., Burden, J. L. and Peberdy, J. F. 1996. Evidence for the association of the enteric bacterium Ewingella americana with internal stipe necrosis of Agaricus bisporus. Microbiol. 142:3255-3260
  8. Inglis, P. W. and Peberdy, J. F. 1996. Evidence of the association of the enteric bacterium Ewingella Americana with internal stipe necrosis of Agaricus bisporus. Microbiol. 142:3253-3260 https://doi.org/10.1099/13500872-142-11-3253
  9. Kumar, S., Tamura, K. and Nei, M. 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform. 5:150-163 https://doi.org/10.1093/bib/5.2.150
  10. Lane, D. J. 1991. 16S/23S rRNA sequencing. In: Nucleic Acid Techniques in Bacterial Systematics, pp. 115-175. Eds. E. Stackebrandt and M. Goodfellow. John Wiley & Sons, New York
  11. Lanyi, B. 1987. Classical and rapid identification methods for medically important bacteria. Methods Microbiol. 19:1-6
  12. Roy Chowdhury P., Pay, J. and Braithwaite, M. 2007. Isolation, identification and ecology of Ewingella americana (the causal agent of internal stipe necrosis) from cultivated mushrooms in New Zealand. Australasian Plant Pathol. 36:424-428 https://doi.org/10.1071/AP07045
  13. Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425
  14. Sasser, M. J. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical note 101. Microbial ID Inc. Newark, DE, USA
  15. Stainer, R. Y., Palleroni, N. J. and Doudoroff, M. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43:159-271 https://doi.org/10.1099/00221287-43-2-159

피인용 문헌

  1. Identification and RAPD-typing of Ewingella americana on cultivated mushrooms in Castilla-La Mancha, Spain vol.133, pp.3, 2012, https://doi.org/10.1007/s10658-012-9952-1