Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Inhibitory effects of Tinospora cordifolia and Rubia cordifolia Linn. on egg albumin-induced experimental allergic conjunctivitis in rats

  • Rishit, Zalawadia (Pharmacy Department, Faculty of Tech. & Engg., Kalabhavan, M. S. University of Baroda) ;
  • Chintan, Gandhi (Pharmacy Department, Faculty of Tech. & Engg., Kalabhavan, M. S. University of Baroda) ;
  • Vaibhav, Patel (Pharmacy Department, Faculty of Tech. & Engg., Kalabhavan, M. S. University of Baroda) ;
  • Balaraman, R (Pharmacy Department, Faculty of Tech. & Engg., Kalabhavan, M. S. University of Baroda)
  • Published : 2009.03.31
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Abstract

Allergic conjunctivitis is the most common allergic disease. These diseases are severe & frequent which requires search of new treatments. The aim of the study was to investigate the effects of Tinospora cordifolia (TC), Rubia cordifolia Linn. (RC) on experimentally induced allergic conjunctivitis in rats. In this study, dried water soluble extracts of TC and RC. (250 and 500 mg/kg, p.o. for 7 days) were evaluated for their antiallergic activity in Wistar rats. They were tested for inhibition of egg albumin-induced vascular permeability, inhibition of histamine release from the rat conjunctiva as well as in histamine content in tears. TC and RC showed significant (P < 0.05) inhibition in vascular permeability, inhibition in histamine release from the rat conjunctiva which is reflected by reduced level of histamine content in tears. The activities were found to be comparable to azelastine hydrochloride. These results suggest that the inhibitory effect on egg albumin-induced experimental allergic conjunctivitis in rat may be due to the antihistaminic activity of TC and RC. Our studies provide evidence that TC and RC may be beneficial in the treatment of allergic conjunctivitis.

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References

  1. Abelson MB, George MA, Garofalo C. (1993) Differential diagnosis of ocular allergic disorders. Ann. Allergy 70, 95-109
  2. Abelson MB, Udell IJ, Allansmith MR, Raizman MB. (1994) Principles and practice of ophthal-mology. In: Allergic and toxic reactions, edited by Albert DM, Jakobiec FA, p.77, W.B. Saunders, Philadelphia
  3. Allansmith MR, Baird RS. (1981) Percentage of degranulated mast cells in vernal conjunctivitis and giant papillary conjunctivitis associated with contact lens wear. Am. J. Ophthalmol. 9, 71-75 https://doi.org/10.1111/j.1442-9071.1981.tb00915.x
  4. Allansmith MR, Ross RN. (1986) Ocular allergy and mast cell stabilizers. Surv. Ophthalmol. 30, 229-234 https://doi.org/10.1016/0039-6257(86)90119-0
  5. Anton AH, Sayre DF. (1969) A modified flurometric procedure for tissue histamine and its distribution in various animals. J. Pharmacol. Exp. Ther. 166, 285-292
  6. Berdy GJ. (1999) Atopic Keratoconjunctivitis. Acta Ophthalmol. Scandinavica 77, 7-9
  7. Bielory L, Buddiga P, Bigelson S. (2004) Ocular allergy treatment comparisons: azelastine and olopatadine. Curr. Allergy Asthma Rep. 4, 320-325 https://doi.org/10.1007/s11882-004-0078-1
  8. Bishayi B, Roychowdhury S, Ghosh S, Sengupta M. (2002) Hepatoprotective and immunomodulatory properties of Tinospora cordifolia in $CCl_4$ intoxicated mature albino rats. J. Toxicol. Sci. 27, 139-146 https://doi.org/10.2131/jts.27.139
  9. Calonge MC, Pastor JC, Herreras JM, Gonzalez JL. (1990) Pharmacologic modulation of vascular permeability in ocular allergy in the rat. Invest. Ophthalmol. Vis. Sci. 31, 176-180
  10. Chand N, Pillar J, Diamantis W, Perhach JLJ, Sofia RD. (1983) Inhibition of calcium ionophore (A23187)-stimulated histamine release from rat peritoneal mast cells by azelastine: implications for its mode of action. Eur. J. Pharmacol. 96, 227-233 https://doi.org/10.1016/0014-2999(83)90311-4
  11. Ciprandi G, Buscaglia S, Catrullo A, Pesce G, Fiorino N, Montagna P. (1997) Azelastine eye drops reduce and prevent allergic conjunctival reaction and exert anti-allergic activity. Clin. Exp. Allergy 27, 182-191 https://doi.org/10.1111/j.1365-2222.1997.tb00691.x
  12. Donshik PC, Ehlers WH. (1994) The Cornea. In: Clinical immunologic diseases, edited by Smolin G, Thoft RA, p. 347, Little, Brown and Company, Boston
  13. Epstein AB. (2002) New horizons in ocular allergy. Rev. Optometry. 139, 117-124
  14. Hamid Q, Tulic' MK, Liu MC, Moqbel R. (2003) Inflammatory cells in asthma: mechanisms and implications for therapy. J. Allergy Clin. Immunol. 111, S5-S12 https://doi.org/10.1067/mai.2003.22
  15. Jorapurkar AA, Deode NM, Zambad SP, Umathe SN. (2003a) Immunomodulatory activity of Rubia cordifolia Linn. Indian Drugs 40, 179-181
  16. Jorapurkar AA, Zambad SP, Wanjari MM, Umathe SN. (2003b) In vivo evaluation of antioxidant activity of alcoholic extract of Rubia cordifolia Linn. and its influence on ethanol-induced immunosuppression. Indian J. Pharmacol. 35, 232-236
  17. Kasture SB, Kasture VS, Chopde CT. (2001) Antiinflammatory activity of Rubia cordifolia roots. J. Nat. Remedies 2, 111-115
  18. Leonardi A. (2000) Role of histamine in allergic conjunctivitis. Acta Ophthalmol. Scand. 230, 18-21 https://doi.org/10.1034/j.1600-0420.2000.078s230018.x
  19. Leonardi A. (1999) Pathophysiology of allergic conjunctivitis. Acta Ophthalmol. Scand. 228, 21-23
  20. Leonardi A, Bloch KJ, Briggs R, Allansmith MR. (1990) Histology of ocular late-phase reaction in guinea pigs passively sensitized with IgG1 antibodies. Ophthalmic Res. 22, 209-219 https://doi.org/10.1159/000267025
  21. McGill JI, Bacon A. (1998) Allergic eye disease mechanisms. Br. J. Pharmacol. 82, 1203-1214
  22. Minami K, Kamei C. (2004) A chronic model for evaluating the itching associated with allergic conjunctivitis in rats. Int. Immunopharmacol. 4, 101-108 https://doi.org/10.1016/j.intimp.2003.10.013
  23. Nakahara H, Izushi K, Sugimoto Y, Watanabe T, Kamei C. (2000) Vascular permeability in allergic conjunctivitis in mice lacking histamine H1 receptors. Eur. J. Pharmacol. 409, 313-317 https://doi.org/10.1016/S0014-2999(00)00863-3
  24. Pendse VK, Mahavar MM, Khanna KC, Somani SK. (1981) Anti-inflammatory and related activity of water extract of Tinospora cordifolia, 'Neem- Giloe'. Indian Drugs 19, 14-21
  25. Petersen LJ, Mosbech H, Skov PS. (1996) Allergeninduced histamine release in intact human skin in vivo assessed by skin microdialysis technique: characterization of factors infuencing histamine releasability. J. Allergy Clin. Immunol. 97, 672-679 https://doi.org/10.1016/S0091-6749(96)70313-5
  26. Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC. (2003) Chemistry and medicinal properties of Tinospora cordifolia (guduchi). Indian J. Pharmacol. 35, 83-91
  27. Stock EL, Pendleton RB. (1993) Pharmacologic treatment of ocular allergic diseases. Int. Ophthalmol. Clin. 33, 47-58 https://doi.org/10.1097/00004397-199303340-00006
  28. Subramanian M, Chintalwar GJ, Chattopadhyay S. (2002) Antioxidant properties of a Tinospora cordifolia polysaccharide against iron-mediated lipid damage and gamma-ray-induced protein damage. Redox Rep. 7, 137-143 https://doi.org/10.1179/135100002125000370
  29. Togias A. (2003) H1-receptors: localization and role in airway physiology and in immune functions. J. Allergy Clin. Immunol. 112, S60-S68 https://doi.org/10.1016/S0091-6749(03)01878-5
  30. Tripathi YB, Muto T, Fukami H, Saito K, Horikawa C, Tsuruoka N, Saito M, Sugiura N, Yamashiro K, Sumida M, Kakutani S, Fukuda Y. (2002) Chymase participates in chronic dermatitis by inducing eosinophil infiltration. Lab. Invest. 82, 789-794
  31. Tripathi YB, Sharma M, Manickam M. (1997) Rubiadin, a new antioxidant from Rubia cardifolia. Indian J. Biochem. Biophys. 3, 302-306
  32. Yanni JM, Stephens DJ, Miller ST, Weimer LK, Graff G, Parnell D. (1996) The in vitro and in vivo ocular pharmacology of olopatadine (AL-4943A), an effective anti-allergic/antihistaminic agent. J. Ocul. Pharmacol. Ther. 4, 389-400 https://doi.org/10.1089/jop.1996.12.389