The expressions of C-reactive protein and macrophage colony-stimulating factor in gingival tissue of human chronic periodontitis with hypertension

고혈압을 동반한 만성 치주염 환자의 치은 조직에서 C-reactive protein과 macrophage colony-stimulating factor의발현

  • Kim, Chul-Woo (Department of Periodontology, School of Dentistry, Kyungpook National University) ;
  • Park, Jin-Woo (Department of Periodontology, School of Dentistry, Kyungpook National University) ;
  • Suh, Jo-Young (Department of Periodontology, School of Dentistry, Kyungpook National University) ;
  • Cho, Je-Yoel (Department of Biochemistry, School of Dentistry, Kyungpook National University) ;
  • Lee, Jae-Mok (Department of Periodontology, School of Dentistry, Kyungpook National University)
  • 김철우 (경북대학교 치의학전문대학원 치주과학교실) ;
  • 박진우 (경북대학교 치의학전문대학원 치주과학교실) ;
  • 서조영 (경북대학교 치의학전문대학원 치주과학교실) ;
  • 조제열 (경북대학교 치의학전문대학원 구강생화학교실) ;
  • 이재목 (경북대학교 치의학전문대학원 치주과학교실)
  • Published : 2009.12.31

Abstract

Purpose: The purpose of this study was to quantify and compare the expressions of CRP and M-CSF in the gingival tissues of the patients with chronic periodontitis associated to hypertension. Methods: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. Clinically healthy gingival tissue samples from systemically healthy 12 patients were categorized as group 1 (n=12). Inflammatory gingival tissue samples from patients with chronic periodontitis were categorized as group 2 (n=12). Inflammatory gingival tissue samples from patients with chronic periodontitis associated with hypertension were categorized as group 3 (n=12). Tissue samples were prepared and analyzed by Western blotting. The quantification of CRP and M-CSF were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. Results: There were significant differences between group 1 and group 2 and between group 1 and group 3 in both CRP and M-CSF. The differences between group 2 and group 3 were not statistically significant in both proteins. However, the expression levels of CRP and M-CSF in hypertensive inflammatory gingiva showed increased tendency compared to non-hypertensive inflammatory gingiva. Conclusions: It is suggested that CRP and M-CSF might be used as inflammatory and bone resorption markers in periodontal diseased tissue. It is assumed that hypertension may be associated with the progression of periodontal inflammation and alveolar bone resorption.

Keywords

References

  1. Shapira L, Soskolne WA, Sela MN, Offenacher S, Barak V. The secretion of FE2, IL-1$\beta$, IL-6 and TNF-$\alpha$ by adherent mononuclear cells from early onset periodontitis patients. J Periodontol 1994;65:139-146 https://doi.org/10.1902/jop.1994.65.2.139
  2. Hernichel-Gorbach E, Kornman KS, Holt SC et al. Host responses in patients with generalized refractory periodontitis. J Periodontol 1994;65:8-16 https://doi.org/10.1902/jop.1994.65.1.8
  3. Page RC. The role of inflammatory mediators in the pathogenesis of periodontal disease. J Periodont Res 1991;26:230-242 https://doi.org/10.1111/j.1600-0765.1991.tb01649.x
  4. Liu D, Xu JK, Figliomeni L et al. Expression of RANKL and OPG mRNA in periodontal disease: Possible involvement in bone destruction. Int J Mol Med 2003;11:17-21
  5. Crotti T, Smith MD, Hirsch R et al. Receptor activator NF kappaB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis. J Periodont Res 2003;38:380-387 https://doi.org/10.1034/j.1600-0765.2003.00615.x
  6. Suda T, Takahashi N, Martin TJ. Modulation of osteoclast differentiation. Endocr Rev 1992;13:6-80
  7. Lacey DL, Timms E, Tan HL et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 1998;93:165-167 https://doi.org/10.1016/S0092-8674(00)81569-X
  8. Katagiri T, Takahashi N. Regulatory mechanism of osteoblast and osteoclast differentiation. Oral Diseases 2000;8:147-159 https://doi.org/10.1034/j.1601-0825.2002.01829.x
  9. Cochran DL. Inflammation and bone loss in periodontal disease. J Periodontol 2008;suppl:1569-1576
  10. Masato K, Masahide M, Motokawa et al. VEGF and M-CSF levels in periodotal tissue during tooth movement. Biomed Res 2008;29:181-187 https://doi.org/10.2220/biomedres.29.181
  11. Steel D, Whitehead AS. The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A protein. Immunol Today 1994;15:81-88 https://doi.org/10.1016/0167-5699(94)90138-4
  12. Baumann H, Gauldie J. The acute phase response. Immunol Today 1994;15:74-80 https://doi.org/10.1016/0167-5699(94)90137-6
  13. Buhin K, Gustafsson A, Pockley AG, Frostegard J, Klinge B. Risk factors for cardiovascular disease in patients with periodontitis. Eur Heart J 2003;24:2099-2107 https://doi.org/10.1016/j.ehj.2003.09.016
  14. Vigushin DM, Pepsy MB, Hawkins PN. Metabolic and scintigraphic studies of radioiodinated human C-reactive protein in health and diseases. J Clin Invest 1993;91:1351-1357 https://doi.org/10.1172/JCI116336
  15. Yeh ETH, Willerson JT. Coming of Age of C-reactive protein using Inflammation markers in cardiology. Circulation 2003;107:370-371 https://doi.org/10.1161/01.CIR.0000053731.05365.5A
  16. Loos BG, Craandijk J, Hoek FJ, Wertheim-van Dillen P, van der Velden. Elevation of systemic markers related to cardiovascular diseases in the peripheral blood of periodontitis patients. J Periodontol 2000;71:1528-1534 https://doi.org/10.1902/jop.2000.71.10.1528
  17. Slade GD, Offenbacher S, Beck JD, Heiss G, Pankow JS. Acute phase inflammatory response to periodontal disease in the US population. J Dent Res 2000;79:49-57 https://doi.org/10.1177/00220345000790010701
  18. Scannapieco FA, Bush RB, Paju S. Associations between periodontal diseases and risk for atherosclerosis, cardiovascular disease, and stroke. Ann Periodontol 2003;8:38-53 https://doi.org/10.1902/annals.2003.8.1.38
  19. Destefano F, Anda RF, Kahn HS, Williamson DF, Russell CR. Dental disease and risk of coronary heart disease and mortality. BMJ 1993;306:688-691 https://doi.org/10.1136/bmj.306.6879.688
  20. Albandar JM, Strekfus CF, Adesanya MR, Winn DM. Cigar, pipe, and cigarette smoking as risk factors for periodontal diseases and tooth loss. J Periodontol 2000;71:1874-1881 https://doi.org/10.1902/jop.2000.71.12.1874
  21. Susin C, Oppermann RV, Haugejorden O, Albandar JM. Periodontal attachment loss attributable to cigarette smoking in an urban Brazilian population. J Clin Periodontol 2004;31:951-958 https://doi.org/10.1111/j.1600-051x.2004.00588.x
  22. Emrich LJ, Shlossman M, Genco RJ. Periodontal disease in non-insulin-dependent diabetes mellitus. J Periodontol 1991;62:123 -131 https://doi.org/10.1902/jop.1991.62.2.123
  23. Bridges RB, Anderso JW, Saxe SR, Greogrory K, Bridge SR. Periodontal status of diabetic and non-diabetic men: effects of smoking, glycemic control, and socioeconomic factors. J Periodontol 1996;67:1185-1192 https://doi.org/10.1902/jop.1996.67.11.1185
  24. Muhlemann HR, Son SH. Gingival sulcus bleeding-a leading symptom in initial gingivitis. Helv Odontol Acta. 1971;15:107-113
  25. Kim DH, Park JW, Lee JM et al. The comparison of MMP-2, MMP-9 and tumor necrosis factor-$\alpha$ expressions in human gingiva with chronic periodontitis with or without associated to type 2 diabetes mellitus. J Korean Aca Periodonto 2006;36:409-426
  26. Cho JY, Xing S, Liu X, Buckwalter TLF et al. Expression and activity of human ${Na}^+$/$I^{-}$ symporter in human glioma cells by adenovirus-mediated gene delivary. Gene Therapy 2000;7:740-749
  27. Frederiksson MI, Figueredo CMS, Gustafsson A, Bergstrom KG, Asman BE. Effect of periodontitis and smoking on blood leukocytes and acute-phase proteins. J Periodontol 1999;70:1355-1360 https://doi.org/10.1902/jop.1999.70.11.1355
  28. Ebersole JL, Machen RL, Steffen MJ, Willmann DE. systemic acute phase reactants, C-reactive protein and haptoglobin in adult periodontitis. Clin Exp Immunol 1997;107:347-352 https://doi.org/10.1111/j.1365-2249.1997.270-ce1162.x
  29. Beck JD, Slade G, Offenbacher S. Oral disease, cardiovascular disease and systemic inflammation. J Periodontol 2000;23:110-120 https://doi.org/10.1034/j.1600-0757.2000.2230111.x
  30. Noack B, Genco R.J, Trevisan M et al. Periodontal infections contribute to elevate systemic C-reactive protein level. J Periodontol 2001;72:1221-1227 https://doi.org/10.1902/jop.2000.72.9.1221
  31. Matilla K, Vesanen M, Valtonen V et al. Effect of treating periodontitis on C-reactive protein levels: A pilot study. BMC Infect Dis 2002;2:30-32 https://doi.org/10.1186/1471-2334-2-30
  32. Ide M, Mcpartin D, Coward PY et al. Effect of treatment of chronic periodontitis on levels of serum markers of acute-phase inflammatory and vascular responses. J Clin Periodontol 2003;30:334-340 https://doi.org/10.1034/j.1600-051X.2003.00282.x
  33. Tussanee Y, Kanokporn L, Prasit P. Human periodontal ligament cells secret macrophage colony-stimulating factor in response to tumor necrosis factor-alpha in vitro. J periodontol 2006;77:955-962 https://doi.org/10.1902/jop.2006.050338
  34. Roberts FA, McCaffery KA, Michalek SM. Profile of cytokine mRNA expression in chronic adult periodontitis. J Dent Res 1997;76:1833-1839 https://doi.org/10.1177/00220345970760120501
  35. Sudha A, Charu SC, Nicholas PP et al. Regulation of periodontal ligament cell functions by interleukin-1 beta. Infect Immun 1998;66:932-937
  36. Castelli WA, Diaz-Perez R, Nasjleti CE, Caffesse RG. Effect of renovascular hypertension of the morphology of oral blood vessels. Oral Surg Oral Med Oral Pathol 1978;46:576-582 https://doi.org/10.1016/0030-4220(78)90388-2
  37. Perlstein MI, Bissada NF. Influence of obesity and hypertension on the severity of periodontitis in rats. Oral Surg Oral Med Oral Pathol 1977;4:707-719
  38. Angeli F, Verdecchia P, Pelligrino C et al. Association between periodontal diseases and left ventricle mass in essential hypertension. Hypertension 2003;41:488-492 https://doi.org/10.1161/01.HYP.0000056525.17476.D7
  39. Ridker PM, Buring JE, Cook NR, Rifai N. C-reactive protein, the metabolic syndrome, and risk of incident cardiovascular events: an 8-year follow up of 1419 initially healthy American women. Circulation 2003;107:391-397 https://doi.org/10.1161/01.CIR.0000055014.62083.05
  40. Lakoski SG, Cushman M, Palmas W et al. The relationship between blood pressure and C-reactive protein in the Multi-Ethnic study of Atherosclerosis (MESA). J Am Coll Cardiol 2005;46:1869-1874 https://doi.org/10.1016/j.jacc.2005.07.050