The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Powdery Mildew Caused by Erysiphe cruciferarum on Arabidopsis thaliana in Korea

  • Choi, Hyong-Woo (Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology) ;
  • Choi, Young-Jun (Laboratory of Fungal Taxonomy and Ecology, College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Dae-Sung (Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology) ;
  • Hwang, In-Sun (Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology) ;
  • Choi, Du-Seok (Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology) ;
  • Kim, Nak-Hyun (Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology) ;
  • Lee, Dong-Hyuk (Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology) ;
  • Shin, Hyeon-Dong (Laboratory of Fungal Taxonomy and Ecology, College of Life Sciences and Biotechnology, Korea University) ;
  • Nam, Jae-Sung (Department of Molecular Biology, Dong-A University) ;
  • Hwang, Byung-Kook (Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology)
  • Published : 2009.03.31
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Abstract

In November 2008, typical powdery mildew symptoms were observed on leaves of Arabidopsis thaliana ecotype Col-0 plants in a growth room under controlled laboratory conditions at Korea University, Seoul. The disease was characterized by the appearance of white powder-like fungal growth on the surface of infected leaves. As the disease progressed, infected leaves exhibited chlorotic or necrotic brown lesions, and leaf distortion and senescence. Conidiophores of the causal fungus were hyaline, unbranched, 3-4 celled, cylindrical, and $80-115{\times}6-9{\mu}m$ in size. Singly produced conidia (pseudoidium type) were hyaline, oblong to cylindrical or oval in shape, and $26-55{\times}15-20{\mu}m$ in size with a length/width ratio of average 3, angular/rectangular wrinkling of outer wall and no distinct fibrosin bodies. Appressoria on the hyphae were multi-lobed. These structures are typical of the powdery mildew Oidium subgenus Pseudoidium, anamorph of the genus Erysiphe. The measurements of the fungal structures coincided with those of Erysiphe cruciferarum. The phylogenetic analysis using ITS rDNA sequences revealed that the causal fungus Erysiphe sp. KUS-F23994 is identical to E. cruciferarum. The isolated fungus incited powdery mildew symptoms on the inoculated Arabidopsis leaves, which proved Koch's postulates. Taken all data together, we first report the occurrence of powdery mildew disease of A. thaliana caused by Erysiphe cruciferarum in Korea.

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References

  1. Adam, L. and Somerville, S. C. 1996. Genetic characterization of five powdery mildew disease resistance loci in Arabidopsis thaliana. Plant J. 9:341-356 https://doi.org/10.1046/j.1365-313X.1996.09030341.x
  2. Allen, R. L., Bittner-Eddy, P. D., Grenville-Briggs, L. J., Meitz, J. C., Rehmany, A. P., Rose, L. E. and Beynon, J. L. 2004. Hostparasite coevolutionary contlict between Arabidopsis and dovmy mildew. Science 306: 1957-1960 https://doi.org/10.1126/science.1104022
  3. Arabidopsis Genome Initiative 2000. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796-815 https://doi.org/10.1038/35048692
  4. Braun, U. 1987. A monograph of the Erysiphales (powdery mildews). Beihefte zurNova Hedwigia No. 89. J. Cramer, Berlin, Gemlany
  5. Ellis, J., Catanzariti, A. M. and Dodds, P. 2006. The problem of how fungal and oomycete avirulence proteins enter plant cells. Trends Plant Sci. 11:61-63 https://doi.org/10.1016/j.tplants.2005.12.008
  6. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the Bootstrap. Evolution 39:783-791 https://doi.org/10.2307/2408678
  7. Glazebrook, J.. Rogers, E. E. and Ausubel, F. M. 1997. Use of Arabidopsis for genetic dissection of plant defense responses. Annu. Rev. Genet. 31 :547-569 https://doi.org/10.1146/annurev.genet.31.1.547
  8. Holub, E. B. 2007. Natural variation in innate immlmity of a pioneer species. Curr. Opin. Plant Biol. 10:415-424 https://doi.org/10.1016/j.pbi.2007.05.003
  9. Hwang, B. K. and Heitefuss, R. 1982. Characterization of adult plant resistance of spring barley to powdery mildew (Erysiphe graminis f sp. hordei). J. Phytopathol. 104: 168-178 https://doi.org/10.1111/j.1439-0434.1982.tb00523.x
  10. Jee, H. J., Shim, C. K., Choi, Y. J. and Shin, H. D. 2008. Powdery mildew caused by Erysiphe cruciferanlln is found for the fIrst time on Chinese cabbage in Korea. Plant PathoI. 57:777
  11. Koch, E. and Slusarenko, A. J. 1990. Fungal pathogens of Arabidopsis thaliana (L.) Heyhn. Bot. Helv. 100:257-268
  12. Mauch-Mani, B. and Slusarenko, A. J. 1993. Arabidopsis as a model host for studying plant-pathogen interactions. Trends Microbiol. 1 :265-270 https://doi.org/10.1016/0966-842X(93)90049-W
  13. Micali, C., millner, K., Hwnphry, M., Consonni, C. and Panstruga, R. 2008. The powdery mildew disease of Arabidopsis: A paradigm for the interaction between plants and biotrophic fungi. The Arabidopsis Book pp. 1-19
  14. Plotnikova, J. M., Reuber. T. L. and Ausubel, F. M. 1998. Powdery mildew pathogenesis on Arabidopsis thaliana. Mycologia 90: 1009-1016 https://doi.org/10.2307/3761274
  15. Sanez, G S. and Taylor, J. W. 1999. Phylogeny of Erysiphales (powdery mildews) inferred from intel11al transcribed spacer (ITS) ribosomal DNA sequences. Can. J. Bot. 77:150-168 https://doi.org/10.1139/cjb-77-1-150
  16. Shin, H. D. and La.. Y. J. 1992. Addition to the new records of host plants of powdery mildews in Korea. Korean J. Plant Palhol. 8:57-60
  17. Takamatsu, S. and Kano, Y. 2001. PCR primers useful for nueleotide sequencing of rDNA of the powdery mildew fungi. Mycoscience 42:135-139 https://doi.org/10.1007/BF02463987
  18. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Bioi. Evol. 24: 1596-1599 https://doi.org/10.1093/molbev/msm092
  19. Tucker, S. L. and Talbot, N. J. 2001. Surface attachment and prepenetration stage development by pathogenic fungi. Annu. Rev. Phytopathol. 39:385-417 https://doi.org/10.1146/annurev.phyto.39.1.385
  20. Vogel, J. and Sommerville, S. 2002. Powdery mildew of Arabidopsis: a model system for host-parasite interactions, In: The Powdery Mildews, a comprehensive treatise. Belanger, R.R., Bushnell, W. R., Dik, A. J., Carver, T. N. W. eds. The Arnerican Phytopathological Society Press. St. Paul. pp. 161-168
  21. White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Arnplication and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols, A Guide to Methods and Application. Innis, M. A., Gelfand, D. H., Sninsky, J. J., White, T. J. eds. Academic Press, London. pp. 315-322
  22. Xiao, S., Ellwood, S., Findlay, K., Oliver, R. P. and Tumer, J. G. 1997. Characterization of three loci controlling resistance of Arabidopsis thaliana accession Ms-O to two powdery mildew diseases. Plant J 12:757-768 https://doi.org/10.1046/j.1365-313X.1997.12040757.x

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