The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Increasing Effects of Apoptosis When Co-treated Scutellaria barbata D. Don. with Anti-cancer Drugs

반지련(半枝蓮)과 항암제 병용 투여에 의한 암세포 성장 저해에 관한 연구

  • Nam, Ju-Young (Dept. of Ob&Gy, College of Korean Medicine, Dongguk Univ.) ;
  • Sung, Jung-Suk (Dept. of Life Science, College of Life Science, Dongguk University) ;
  • Jun, Hyun-Ik (Dept. of Life Science, College of Life Science, Dongguk University) ;
  • Lee, Jeong-Won (Dept. of Ob&Gy, College of Korean Medicine, Dongguk Univ.) ;
  • Kwon, Su-Kyung (Dept. of Ob&Gy, College of Korean Medicine, Dongguk Univ.) ;
  • Kim, Dong-Il (Dept. of Ob&Gy, College of Korean Medicine, Dongguk Univ.)
  • 남주영 (동국대학교 한의과대학 부인과학교실) ;
  • 성정석 (동국대학교 생명과학대학 생명과학과) ;
  • 전현익 (동국대학교 생명과학대학 생명과학과) ;
  • 이정원 (동국대학교 한의과대학 부인과학교실) ;
  • 권수경 (동국대학교 한의과대학 부인과학교실) ;
  • 김동일 (동국대학교 한의과대학 부인과학교실)
  • Published : 2009.02.27
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Abstract

Purpose: This experiment was designed to find out increasing effects of S. barbata. co-treatment with anti-cancer drugs at cancer cell's growth inhibition effect. Methods: Divergent observational study of the S. barbata. co-treatment with Cisplatin treatment on HeLa cell. Cell viability using MTT assay, Cell Culture and Cytotoxicity Studies, Cell Cycle Analysis, Annexin V-FITC/PI assay, Cell morphological assessment, PARP cleavage using Western blotting analysis when HeLa cell were co-treated with Cisplatin and Scutellaria Barbata extracts. Results: When HeLa cell were co-treated with Cisplatin and Scutellaria Barbata extracts, we found out viability of HeLa cell, changing in the distribution of cell cycle, Annexin V-FITC staining, DAPI staining, PARP clavage protein assay by Western-blot. So Scutellaria Barbata extracts have increased apoptosis Conclusion: When co-treated Scutellaria Barbata extracts with anti-cancer drugs, the anti-cancer effects were increased. We still not sure which constituent apoptosis at cancer cells and activates anti-cancer effects suppressing, but we believe that it'll be revealed here after with following experiments.

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References

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