Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Basic Fibroblast Growth Factor Increases Intracellular Magnesium Concentration through the Specific Signaling Pathways

  • Hong, Bing-Zhe (Department of Pharmacology, Chonbuk National University Medical School) ;
  • Park, Sun-Ah (Department of Pharmacology, Chonbuk National University Medical School) ;
  • Kim, Han-Na (Department of Pharmacology, Chonbuk National University Medical School) ;
  • Ma, Tian-Ze (Department of Pharmacology, Chonbuk National University Medical School) ;
  • Kim, Han-Gyu (Department of Pharmacology, Chonbuk National University Medical School) ;
  • Kang, Hyung-Sub (Department of Pharmacology, Chonbuk National University College of Veterinary Medicine) ;
  • Kim, Hwan-Gyu (Division of Biological Sciences, Chonbuk National University) ;
  • Kwak, Yong-Geun (Department of Pharmacology, Chonbuk National University Medical School)
  • Received : 2009.04.01
  • Accepted : 2009.06.08
  • Published : 2009.07.31
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Abstract

Basic fibroblast growth factor (bFGF) plays an important role in angiogenesis. However, the underlying mechanisms are not clear. $Mg^{2+}$ is the most abundant intracellular divalent cation in the body and plays critical roles in many cell functions. We investigated the effect of bFGF on the intracellular $Mg^{2+}$ concentration ($[Mg^{2+}]_i$) in human umbilical vein endothelial cells (HUVECs). bFGF increased ($[Mg^{2+}]_i$) in a dose-dependent manner, independent of extracellular $Mg^{2+}$. This bFGF-induced $[Mg^{2+}]_i$ increase was blocked by tyrosine kinase inhibitors (tyrphostin A-23 and genistein), phosphatidylinositol 3-kinase (PI3K) inhibitors (wortmannin and LY294002) and a phospholipase $C{\gamma}$ ($PLC{\gamma}$) inhibitor (U73122). In contrast, mitogen-activated protein kinase inhibitors (SB202190 and PD98059) did not affect the bFGF-induced $[Mg^{2+}]_i$ increase. These results suggest that bFGF increases the $[Mg^{2+}]_i$ from the intracellular $Mg^{2+}$ stores through the tyrosine kinase/PI3K/$PLC{\gamma}$-dependent signaling pathways.

Keywords

Acknowledgement

Supported by : Korea Research Foundation

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