대한수의학회지 (Korean Journal of Veterinary Research)

  • 제49권1호
  • /
  • Pages.59-62
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  • 2009
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

Single-nucleotide polymorphisms in prion protein gene of the Korean subspecies of Chinese water deer

  • 심사 : 2009.02.25
  • 발행 : 2009.03.30
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초록

Susceptibility to chronic wasting disease (CWD) in cervid species has been associated with polymorphisms in the prion protein gene (PRNP). The single nucleotide polymorphisms (SNPs) were found in the PRNP of the Korean subspecies of Chinese water deer via analyses of the DNA sequences obtained from 34 individual deer. Two SNPs were detected at codons 77 and 100. One SNP at codon 77 encoding Glycine was determined to be a silent mutation but the other SNP detected at codon 100 induced an amino acid change, from Asparagine to Serine. The prion protein (PrP) amino acid sequence of the water deer showed 98.8-99.2% homology with those of American elk, white-tailed deer and mule deer. The PrP of the water deer contained amino acid residues closely related with CWD-susceptibility. This study is the first to describe genetic variations in the PRNP of the Korean subspecies of Chinese water deer.

키워드

참고문헌

  1. Brayton KA, O'Rourke KI, Lyda AK, Miller MW,Knowles DP Jr. A processed pseudogene contributesto apparent mule deer prion gene heterogeneity. Gene 2004, 326, 167-173 https://doi.org/10.1016/j.gene.2003.10.022
  2. Collinge J, Sidle KC, Meads J, Ironside J, Hill AF.Molecular analysis of prion strain variation and theaetiology of 'new variant' CJD. Nature 1996, 383, 685-690 https://doi.org/10.1038/383685a0
  3. Hamir AN, Gidlewski T, Spraker TR, Miller JM,Creekmore L, Crocheck M, Cline T, O'Rourke KI.Preliminary observations of genetic susceptibility of elk(Cervus elaphus nelsoni) to chronic wasting disease by experimental oral inoculation. J Vet Diagn Invest 2006,18, 110-114 https://doi.org/10.1177/104063870601800118
  4. Hunter N. PrP genetics in sheep and the implicationsfor scrapie and BSE. Trends Microbiol 1997, 5, 331-334 https://doi.org/10.1016/S0966-842X(97)01081-0
  5. Jewell JE, Conner MM, Wolfe LL, Miller MW,Williams ES. Low frequency of PrP genotype 225SFamong free-ranging mule deer (Odocoileus hemionus)with chronic wasting disease. J Gen Virol 2005, 86,2127-2134 https://doi.org/10.1099/vir.0.81077-0
  6. Johnson C, Johnson J, Clayton M, McKenzie D,Aiken J. Prion protein gene heterogeneity in freeranging white-tailed deer within the chronic wasting disease affected region of Wisconsin. J Wildl Dis 2003,39, 576-581 https://doi.org/10.7589/0090-3558-39.3.576
  7. Johnson C, Johnson J, Vanderloo JP, Keane D,Aiken JM, McKenzie D. Prion protein polymorphismsin white-tailed deer influence susceptibility to chronicwasting disease. J Gen Virol 2006, 87, 2109-2114 https://doi.org/10.1099/vir.0.81615-0
  8. Mathiason CK, Powers JG, Dahmes SJ, Osborn DA,Miller KV, Warren RJ, Mason GL, Hays SA, Hayes-Klug J, Seelig DM, Wild MA, Wolfe LL, SprakerTR, Miller MW, Sigurdson CJ, Telling GC, Hoover EA. Infectious prions in the saliva and blood of deerwith chronic wasting disease. Science 2006, 314, 133-136 https://doi.org/10.1126/science.1132661
  9. Miller MW, Williams ES, McCarty CW, SprakerTR, Kreeger TJ, Larsen CT, Thorne ET. Epizootiology of chronic wasting disease in free rangingcervids in Colorado and Wyoming. J Wildl Dis 2000, 36, 676-690 https://doi.org/10.7589/0090-3558-36.4.676
  10. O'Rourke KI, Besser TE, Miller MW, Cline TF,Spraker TR, Jenny AL, Wild MA, Zebarth GL,Williams ES. PrP genotypes of captive and freerangingRocky Mountain elk (Cervus elaphus nelsoni) with chronic wasting disease. J Gen Virol 1999, 80,2765-2769 https://doi.org/10.1099/0022-1317-80-10-2765
  11. O'Rourke KI, Spraker TR, Hamburg LK, BesserTE, Brayton KA, Knowles DP. Polymorphisms in theprion precursor functional gene but not the pseudogeneare associated with susceptibility to chronic wastingdisease in white-tailed deer. J Gen Virol 2004, 85,1339-1346 https://doi.org/10.1099/vir.0.79785-0
  12. Williams ES. Chronic wasting disease. Vet Pathol 2005,42, 530-549 https://doi.org/10.1354/vp.42-5-530