구강회복응용과학지 (Journal of Dental Rehabilitation and Applied Science)

  • 제25권4호
  • /
  • Pages.361-374
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  • 2009
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  • 2384-4353(pISSN)
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  • 2384-4272(eISSN)
대한턱관절교합학회 (Korean Academy of Stomatognathic Function and Occlusion)
권호 표지

PSII를 이용한 마그네슘 이온 주입 임플란트에 대한 MC3T3-E1 골모양 세포 반응 연구

Cell study on the Magnesium ion implanted surface with PSII

  • 신형주 (강릉원주대학교 치과대학 보철학교실 및 구강과학연구소) ;
  • 김대곤 (강릉원주대학교 치과대학 보철학교실 및 구강과학연구소) ;
  • 박찬진 (강릉원주대학교 치과대학 보철학교실 및 구강과학연구소) ;
  • 조리라 (강릉원주대학교 치과대학 보철학교실 및 구강과학연구소) ;
  • 이희수 (강릉원주대학교 치과대학 구강해부학교실 및 구강과학연구소) ;
  • 차민상 (울산대학교 의과대학 강릉아산병원 치과보철학교실)
  • Shin, Hyeong-Joo (Department of Prsothodontics, College of Dentistry, Gangneung-Wonju National University) ;
  • Kim, Dae-Gon (Department of Prsothodontics, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Chan-Jin (Department of Prsothodontics, College of Dentistry, Gangneung-Wonju National University) ;
  • Cho, Lee-Ra (Department of Prsothodontics, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Hee-Su (Department of oral Anatomy, College of Dentistry, Gangneung-Wonju National University) ;
  • Cha, Min-Sang (Department of Prosthetic Dentistry, Gangneung Asan Hospital, College of Medicine, University of Ulsan)
  • 투고 : 2009.09.25
  • 심사 : 2009.12.25
  • 발행 : 2009.12.31
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초록

임플란트와 골 반응을 개선하기 위한 생화학적 표면 처리 방법으로 다양한 이온을 이용한 이온주입법에 대한 관심이 높아지고 있다. 본 연구는 플라즈마 상태의 마그네슘 이온을 임플란트 표면에 주입하여 이온 피막을 형성하는 방법으로 표면 처리를 한 임플란트에 대한 MC3T3-E1 골모양 세포의 초기 반응을 평가해 보고자 하였다. 티타늄 디스크를 네 가지 군으로 표면처리를 달리하였다. A군은 연마만 하였고 B군은 연마 후 마그네슘 이온을 주입하였다. C군은 알루미늄 입자분사 하였고, D군은 알루미늄 입자분사 후 마그네슘 이온을 주입하였다. 조골세포의 반응을 세포 부착, 증식, 분화의 단계별로 평가하였다. 세포 부착을 평가하기 위해 MC3T3-E1 골모양 세포를 4시간, 24시간, 48시간 금속 표면에서 배양하여 주사현미경으로 관찰하였다. 세포분화도평가는 세포를 4일간 배양 후 알칼리성 인산분해효소 활성도 분석을 통해 시행하였다. 세포외기질의 세포내 발현은 RT-PCR을 통해 평가하였다. 이상의 실험에서 다음과 같은 결과를 얻었다. 1. 주사현미경 관찰시 시간의 흐름에 따라 세포 부착량은 증가하였으며, 마그네슘 이온을 주입한 시편에서 더 많은 양 세포 증식이 관찰되었으며 분화정도도 더 높은 것으로 관찰되었다. 2. RT-PCR 분석시 알루미늄 입자분사 후 마그네슘 이온을 주입한 시편에서 c-fos와 osteonectin의 발현이 증가된 소견을 보였다. 3. 알칼리성 인산분해효소 활성도 분석시 금속 표면처리 방법에 따른 차이는 발생하지 않았다. 이상의 결과를 종합하면 Mg 이온이 주입된 군의 세포가 Mg 이온이 주입되지 않은 군보다 초기의 세포반응이 더 우수하다는 것을 알 수 있다.

For successful osteogenesis around the implants, interaction between implant surface and surrounding tissue is important. Biomechanical bonding and biochemical bonding are considered to influence the response of adherent cells. But the focus has shifted surface chemistry. The purpose of this study is to evaluate the MC3T3-E1 osteoblast like cell responses of magnesium (Mg) ion implanted titanium surface produced using a plasma source ion implantation method. Commercially pure titanium disc was used as substrates. The discs were prepared to produce four different surface, A: Machine turned surface, B: Mg implanted surface, C: sandblasted surface, D: sandblasted and Mg implanted surface. MC3T3 El osteoblastic like cells were cultured on the disc specimens. Cell adhesion, proliferation, differentiation, and synthesis of extracellular matrix were evaluated. The cell adhesion morphology was evaluated by SEM. RT PCR assay was used for assessment of cell adhesion, proliferation and differentiation. ALP activity was measured for cell differentiation. The results of this study were as follows: 1. SEM showed that cell on Mg ion groups was more proliferative than that of non Mg ion groups. On the machine turned surface, cell showed some degree of contact guidance in aligning with the machining grooves. 2. In RT PCR analysis, osteonectin and c-fos mRNA were more expressed on sandblasted and Mg ion implanted group. 3. ALP activity was not significantly different among all groups. Within the limitations of this study, the following conclusions were drawn: It might indicate Mg ion implanted titanium surface induce better bone response than non Mg ion groups.

키워드

과제정보

연구 과제 주관 기관 : 강릉대학교

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