Molecules and Cells

  • 제27권1호
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  • Pages.83-88
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  • 2009
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Characterization of RbmD (Glycosyltransferase in Ribostamycin Gene Cluster) through Neomycin Production Reconstituted from the Engineered Streptomyces fradiae BS1

  • Nepal, Keshav Kumar (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University) ;
  • Oh, Tae-Jin (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University) ;
  • Subba, Bimala (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University) ;
  • Yoo, Jin Cheol (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Sohng, Jae Kyung (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University)
  • 투고 : 2008.09.10
  • 심사 : 2008.10.16
  • 발행 : 2009.01.31
⟨ 이전 논문 다음 논문 ⟩

초록

Amino acid homology analysis predicted that rbmD, a putative glycosyltransferase from Streptomyces ribosidificus ATCC 21294, has the highest homology with neoD in neomycin biosynthesis. S. fradiae BS1, in which the production of neomycin was abolished, was generated by disruption of the neoD gene in the neomycin producer S. fradiae. The restoration of neomycin by self complementation suggested that there was no polar effect in the mutant. In addition, S. fradiae BS6 was created with complementation by rbmD in S. fradiae BS1, and secondary metabolite analysis by ESI/MS, LC/MS and MS/MS showed the restoration of neomycin production in S. fradiae BS6. These gene inactivation and complementation studies suggested that, like neoD, rbmD functions as a 2-N-acetlyglucosaminyltransferase and demonstrated the potential for the generation of novel aminoglycoside antibiotics using glycosyltransferases in vivo.

키워드

과제정보

연구 과제 주관 기관 : Ministry of Commerce, Industry, and Energy

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피인용 문헌

  1. Heterologous production of paromamine in Streptomyces lividans TK24 using kanamycin biosynthetic genes from Streptomyces kanamyceticus ATCC12853 vol.27, pp.5, 2009, https://doi.org/10.1007/s10059-009-0080-5
  2. Heterologous production of ribostamycin derivatives in engineered Escherichia coli vol.161, pp.7, 2009, https://doi.org/10.1016/j.resmic.2010.04.013
  3. Biosynthesis of Ribostamycin Derivatives by Reconstitution and Heterologous Expression of Required Gene Sets vol.163, pp.3, 2009, https://doi.org/10.1007/s12010-010-9045-6
  4. 2-Deoxystreptamine-containing aminoglycoside antibiotics: Recent advances in the characterization and manipulation of their biosynthetic pathways vol.30, pp.1, 2009, https://doi.org/10.1039/c2np20092a
  5. Paired-termini antisense RNA mediated inhibition of DoxR in Streptomyces peucetius ATCC 27952 vol.20, pp.3, 2009, https://doi.org/10.1007/s12257-014-0810-1