Archives of Plastic Surgery

  • 제36권3호
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  • Pages.247-253
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  • 2009
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  • 2234-6163(pISSN)
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  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
권호 표지

콜라겐 코팅된 결정화 하이드록시아파타이트 담체를 이용한 골아세포의 성장

The Effect of Collagen Coating in Crystallized Hydroxyapatite Scaffold

  • 김태곤 (영남대학교 의과대학 성형외과학 교실) ;
  • 박원용 (영남대학교 의과대학 성형외과학 교실) ;
  • 김용하 (영남대학교 의과대학 성형외과학 교실) ;
  • 김연정 (안동대학교 자연대학 응용화학과) ;
  • 최식영 (안동대학교 자연대학 응용화학과)
  • Kim, Tae-Gon (Department of Plastic & Reconstructive Surgery, College of Medicine Yeungnam University) ;
  • Park, Won-Yong (Department of Plastic & Reconstructive Surgery, College of Medicine Yeungnam University) ;
  • Kim, Yong-Ha (Department of Plastic & Reconstructive Surgery, College of Medicine Yeungnam University) ;
  • Kim, Youn-Jung (Applied Chemistry, Natural Science, Andong National University) ;
  • Choi, Sik-Young (Applied Chemistry, Natural Science, Andong National University)
  • 발행 : 2009.05.15
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose: Hydroxyapatite(HA) has been widely used due to its chemical similarity to bone and good biocompatibility. HA is composed of macropores and micropores. Too much irregularities of the micropores are ineffective against the adhesion and proliferation of osteoblast. Many efforts have been tried to overcome these drawbacks. HA crystal coating on the irregular surface of HA scaffold, crystallized HA, is one of the method to improve cell adhesion. Meanwhile, the collagen has been incorporated with HA to create composite scaffold that chemically resembles the natural extracellular matrix components of bone. The authors proposed to examine the effect of collagen - coated crystallized HA on the adhesion and proliferation of osteoblast. Method: HA powder containing $10{\mu}m$ pore size was manufactured as 1 cm pellet size. For the making crystallized HA, 0.1 M EDTA solution was used to dissolve HA powder and heated $100^{\circ}C$ for 48 hours. Next, the crystallized HA pellets were coated with collagen (0.1, 0.5, and 1%). The osteoblasts were seeded into HA pellets and incubated for the various times (1, 5, and 9 days). After the indicating days, methylthiazol tetrazolium (MTT) assay was performed for cell proliferation and alkaline phosphatase (ALP) activty was measured for bone formation. Result: In SEM study, the surface of crystallized HA pellet was more regular than HA pellet. MTT assay showed that the proliferation of osteoblasts increased in a collagen dose - dependent and time - dependent manner and had a maximum effect at 1% collagen concentration. ALP activity also increased in a collagen dose - dependent manner and had a highest effect at 1% collagen concentration. Conclusion: These data showed that crystallization and collagen coating of HA was effective for osteoblast proliferation and ALP activity. Therefore, our results suggest that crystallized - HA scaffold with collagen coating is may be a good strategy for tissue engineering application for bone formation.

키워드

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