Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
권호 표지

Enhancing Enzymatic Saccharification of Corn Stover by Aqueous Ammonia Soaking Pretreatment

옥수수 줄기의 암모니아수 침지 전처리에 의한 효소 당화 향상

  • Shin, Soo-Jeong (Chungbuk National University, College of Agriculture, Life & Environment Sciences, Dept. of Wood and Paper Science) ;
  • Yu, Ju-Hyun (Korea Research Institute of Chemical Technology, Chemical Biotechnology Research Center) ;
  • Cho, Nam-Seok (Chungbuk National University, College of Agriculture, Life & Environment Sciences, Dept. of Wood and Paper Science) ;
  • Han, Sim-Hee (Dept. of Forest Resources Development, Korea Forest Research Institute) ;
  • Kim, Mun-Sung (Chungbuk National University, College of Agriculture, Life & Environment Sciences, Dept. of Wood and Paper Science) ;
  • Park, Jong-Moon (Chungbuk National University, College of Agriculture, Life & Environment Sciences, Dept. of Wood and Paper Science)
  • 신수정 (충북대학교 농업생명환경대학 목재.종이과학전공) ;
  • 유주현 (한국화학연구원 신화학연구단 바이오정밀화학 센터) ;
  • 조남석 (충북대학교 농업생명환경대학 목재.종이과학전공) ;
  • 한심희 (국립산림과학원 산림자원육성부 산림유전자원과) ;
  • 김문성 (충북대학교 농업생명환경대학 목재.종이과학전공) ;
  • 박종문 (충북대학교 농업생명환경대학 목재.종이과학전공)
  • Received : 2009.02.23
  • Accepted : 2009.05.12
  • Published : 2009.07.25
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Abstract

Enhancing enzymatic saccharification of corn stover by aqueous ammonia soaking pretreatment was investigated on chemical compositional changes and enzymatic hydrolysis characteristics. At three different levels of aqueous ammonia soaking temperature and time ($140^{\circ}C$-1 h, $90^{\circ}C$-16 h and $50^{\circ}C$-6 days), higher temperature and shorter treatment time led to more xylan and lignin removal based on overall composition analysis and carbohydrate compositional analysis. More xylan and lignin removal in higher temperature treatment led to higher enzymatic saccharification of cellulose and xylan to monosaccharide by commercial cellulase mixtures (Celluclast 1.5L and Novozym 342 from Novozyme, Denmark).

본 연구는 암모니아수 침지 처리가 옥수수대의 화학 조성과 효소당화 특성에 미치는 영향에 대하여 연구한 것이다. 암모니아수 침지에서 처리 온도와 시간이 본 연구에서 주요 변수였다. 무 처리 분말과 침지 처리 분말의 전체 조성 분석과 탄수화물 조성 분석 결과, 3가지 다른 수준의 암모니아수 침지 조건($140^{\circ}C$ - 1 h, $90^{\circ}C$ - 16h 및 $50^{\circ}C$ - 6일)에서 고온 단기간 처리가 더 많은 양의 자이란과 리그닌을 용출시켰다. 암모니아 침지에 의한 자일란과 리그닌 용출 제거 효과가 고온 침지 처리 된 옥수수 줄기 분말에서 상업용 셀룰라아제를 사용하는 셀룰로오스와 자이란의 효소 당화를 촉진시켰다.

Keywords

Acknowledgement

Supported by : 학술진흥재단

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