한국어병학회지 (Journal of fish pathology)

  • 제22권3호
  • /
  • Pages.239-251
  • /
  • 2009
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

배양 조건이 Photobacterium damselae subsp. damselae의 미생물학적 성상 및 병원성에 미치는 영향

Effects of the microbiological properties and pathogenicity of Photobacterium damselae subsp. damselae under different culture conditions

  • 권문경 (국립수산과학원 병리연구과) ;
  • 조병열 (국립수산물품질검사원 인천지원) ;
  • 박명애 (국립수산과학원 병리연구과)
  • Kwon, Mun-Gyeong (Pathology Division, National Fisheries Research Institute) ;
  • Cho, Byoung-Youl (Incheon Branch Office, National Fisheries Products Inspection Service) ;
  • Park, Myeong-Ae (Pathology Division, National Fisheries Research Institute)
  • 발행 : 2009.12.01
⟨ 이전 논문 다음 논문 ⟩

초록

배양 온도, 배양 배지 중의 pH, NaCl 농도, iron 농도와 같은 배양 조건이 P. damselae의 미생물학적 성상 및 병원성에 미치는 영향을 조사하였다. P. damselae는 배양 온도 15-30${^{\circ}C}$에서 배지의 pH 5-9, NaCl 농도 0-6%와 $FeCl_{3}$ 첨가시 성장이 촉진되었지만, iron-chelator 첨가 시성장이 억제되었다. Iron-limited 조건에서 배양시 ECPs protein 농도, phospholipase, 용혈능, siderophore 및 cytotoxicity 활성 이 높게 나타났고, 넙치 혈청의 살균작용 대한 생존율도 증가 되었으나, iron-added 조건에서는 감소되어, 배양 조건 중 iron 농도에 영향을 가장 많이 받는 것으로 나타났다. 따라서, 본 연구에서 적용한 iron-limited 조건은 철농도가 낮은 숙주 내와 유사한 환경이므로 iron-limited에서 P. damselae 배양 시 나타나는 병원성의 증가는 어체에 감염시 나타나는 병원성 발현 기전과 유사할 것으로 사료된다.

The effects of microbiological properties and pathogenicity of Photobacterium damselae subsp. damselae were investigated under different culture conditions, temperature, pH, NaCl and iron concentration on culture media. Favorable conditions for bacterial growth were between 15-30${^{\circ}C}$, pH 5-9, 0-4% NaCl concentration and iron contents of over 10 mM, whereas the bacterial growth was inhibited under iron chelator existence. When P. damselae was cultured in iron-limited tryptic soy broth, total protein concentration of extracellular products, cytotoxic ability of ECPs on cell line, bacterial viability in flounder serum, phospholipase and siderophore activities of ECPs were significantly increased. On the other hand, the activities of P. damselae cultured under iron-added conditions were decreased. In this study, the iron-limited conditions were similar to the host in which iron concentration is low. During infection caused by P. damselae, the conditions could be related to the pathogenesis of the pathogen.

키워드

참고문헌

  1. Abeck, D., Johnson, A.P., Korting, H.C., Stolz, W., Struck-Suer, E. and Braun-Falco, O.: Effect of iron limitation on protein composition and ultrastructure of Haemophilus ducreyi. FEMS Microbiol. Lett., 68:41-44, 1990 https://doi.org/10.1016/0378-1097(90)90121-6
  2. Austin, B., Austin, D.A., Dalsgaar, I., Gudmundsd Ottir, B.K., Thornton, S.H., Larsen, J.L., O'Hici, B. and Powell, R.: Characterization of atypical Aeromonas salmonicida by different methods. Syst. Appl. Microbiol., 21: 50-64, 1998
  3. Brown, M.R., Anwar, H. and Costerton, J.W.: Surface antigen in vivo; a mirror for vaccine development. Can. J. Microbiol., 39:491-498, 1988
  4. Brown, M.R. and Williams, P.: The influence of environment on envelope properties affecting survival of bacteria in infections. Ann. Rev. Microbiol., 39:527-556, 1985 https://doi.org/10.1146/annurev.mi.39.100185.002523
  5. Chart, H. and Rowe, B.: Iron restriction and growth of Salmonella enteritidis. Epidemiol. Infect. 110:41-47, 1993 https://doi.org/10.1017/S0950268800050664
  6. Colquhoun, D.J., Alvheim, K., Dommarsnes, K., Syvertsen, C. and Sorum, H.: Relevance of incubation temperature for Vibrio salmonicida vaccine production. J. Appl. Microbiol., 92:1087-1096, 2002 https://doi.org/10.1046/j.1365-2672.2002.01643.x
  7. Davies, R.L., Gibbs, H.A., McCluskey, J., Coote, J.G., Freer, J.H. and Parton, R.: Development of an intraperitoneal implant chamber for the study of in vivo-grown Pasteurella haemolytica in cattle. Microbial Pathogenesis, 16:423-433, 1994 https://doi.org/10.1006/mpat.1994.1042
  8. Duncan, D.B.: Multiple-range and multiple F tests. Biometrics, 11:1-42, 1955 https://doi.org/10.2307/3001478
  9. Fernandez-Beros, M.E., Gonzalez, C., McIntosh, M.A. and Cabello, F.C.: Immune response to the iron-deprivation-induced protein of Salmonella typhi in thyphoid fever. Infect. Immun., 57:1271-1275, 1989
  10. Ferrala, N.F., Champlin, A.K. and Fekete, F.A.: Morphological differences in the capsular polysaccharide of nitrogen-fixing Azotobacter chroococcum B-8 as a function of iron and molybdenum. FEMS Microbiol., 33:137-142, 1986 https://doi.org/10.1016/0378-1097(86)90201-6
  11. Fouz, B., Toranzo, A.E., Biosca, E.G., Mazoy, R. and Amro, C.: Role of iron in the pathogenicity of Vibrio damsela for fish and mammals. FEMS Microbiol. Lett., 121:181-188, 1994 https://doi.org/10.1111/j.1574-6968.1994.tb07097.x
  12. Fouz, B., Novoa, B., Troanzo, A.E. and Figueras, A.: Histopathological lesions caused by Vibrio damsela in cultured turbot, Scophthalmus maximus(L.): inoculations with live cells and extracellular products. J. Fish Dis., 18:357-364, 1995 https://doi.org/10.1111/j.1365-2761.1995.tb00312.x
  13. Fouz, B., Barja, J.L., Rivas, C., Amaro, C. and Toranzo, A.E.: Toxicity of the extracellular products of Vibrio damsela isolates from diseased fish. Curr. Microbiol., 27:341-347, 1993 https://doi.org/10.1007/BF01568958
  14. Fouz, B., Larsen, J.L., Nielsen, B., Barja, J.L. and Toranzo, A.E.: Characterization of Vibrio damsela strains isolated from turbot Scophthalmus maximus in Spain. Dis. Aquat. Org., 12:155-166, 1992 https://doi.org/10.3354/dao012155
  15. Fujioka, R.S., Greco, S.B., Cates, M.B. and Schroeder, J.P.: Vibrio damsela from wounds in bottlenose dolphins Tursiops truncatus. Dis. Aquat. Org., 4:1-8, 1988 https://doi.org/10.3354/dao004001
  16. Garduno, R.A., Thornton, J.C. and Kay, W.W.: Fate of the fish pathogen Aeromonas salmonicida in the peritoneal cavity of rainbow trout. Can. J. Microbiol., 39:1051-1058, 1993a https://doi.org/10.1139/m93-159
  17. Garduno, R.A., Thornton, J.C. and Kay, W.W.: Aeromonas salmonicida grown in vivo. Infect. Immun., 61:3854-3862, 1993b
  18. Ho, W.-L. and Chou, C.-C.: Effects of carbon and nitrogen sources, sodium chloride and culture conditions on cytotoxin production by Salmonella choleraesus. Inter. J. Food Microbiol., 67:81-88, 2001 https://doi.org/10.1016/S0168-1605(01)00439-1
  19. Ho, W.-L., Yu, R.-C. and Chou, C.-C.: Effect of iron limitation on the growth and cytotoxin production of Salmonella choleraesuis SC- 5. Inter. J. Food Microbiol., 90:295-302, 2004 https://doi.org/10.1016/S0168-1605(03)00316-7
  20. Jacques, M., Belanger, M., Diarra, M.S., Dargis, M. and Malouin, F.: Modulation of Pasteurella multocida capsular polysaccharide during growth under iron-restricted condition and in vivo. Microbiol., 140:263-270, 1994 https://doi.org/10.1099/13500872-140-2-263
  21. Jepsen, P.K., Riise, E., Biedermann, K., Kristensen, P.C. and Emberg, C.: Two-level factrial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease. Appl. Environ. Microbiol., 53:2593-2596, 1987
  22. Kreger, A.S.: Cytolytic activity and virulence of Vibrio damsela. Infect. Immun., 44:326-331, 1984
  23. Kreger, A.S., Bernheimer, A.W., Etkin, L.A. and Daniel, L.W.: Phospholipase D activity of Vibrio damsela cytolysin and its interaction with sheep erythrocytes. Infect. Immun., 55:3209-3212, 1987
  24. Litwin, C.M. and Calderwood, S.B.: Role of iron in regulation of virulence genes. Clin. Microbiol. Rev., 6:137-159, 1993
  25. Liu, P.C., Lee, K.K. and Chen, S.N.: Pathogenicity of different isolates of Vibrio harveyi from diseased Kuruma prawns Penaeus japonicus. Curr. Microbiol., 33:129-132, 1996 https://doi.org/10.1007/s002849900087
  26. Love, M., Fisher, D.T., Horse, J.E., Farmer, J.J., Hickman, F.W. and Fanning, G.R.: Vibrio damsela, as a marine bacterium, causes skin ulcers on the damselfish Chromis punctipinnis. Science, 214:1140-1141, 1981 https://doi.org/10.1126/science.214.4525.1139
  27. Miles, A.A. and Misra, S.S.: The estimate of the bactericidal power of the blood. J. Hygiene, 38:873-885, 1938 https://doi.org/10.1017/S002217240001158X
  28. Poole, K. and Braun, V.: Influence of growth temperature and lipopolysaccharide on hemolytic activity of Serratia marcescens. J. Bacteriol., 170:5146-5152, 1988
  29. Sakata, T., Matsuura, M. and Shimokawa, Y.: Characteristics of Vibrio damsela isolated from diseased yellowtail(Seriola quinqueradiata). Nippon Suisan Gakkashi, 55:135-141, 1989 https://doi.org/10.2331/suisan.55.135
  30. Schwyn, B. and Neilands, J.B.: Universal chemical assay for the detection an determination of siderophores. Anal. Biochem. 160:46-56, 1987 https://doi.org/10.1016/0003-2697(87)90612-9
  31. SPSS Inc.: SPSS Base 7.5 for Window, SPSS Inc., 444N. Michigan Avenue, Chicago, IL, 60611, 1997
  32. Truper, H.G. and De'Clari, L.: Taxonomic note: necessary correction of specific epithets formed as substantives (nouns) 'in apposition', Int. J. System. Bacteriol., 41:529-534, 1997
  33. Vera, P., Navas, J.L. and Fouz, B.: First isolation of Vibrio damsela from seabream(Sparus aurata). Bull. Eur. Assoc. Fish Pathol., 11: 112-113, 1991
  34. Weinberg, E.D.: Iron withholding: a defense against infection and neoplasia. Physiol. Rev., 64:65-102, 1984
  35. Wong, H.C. and Lee, Y.S.: Effect of iron on the recovery of viable bacteria from the cardiac blood in an experimental mouse model and on the serum resistance and macrophage phagocytosis of Vibrio parahaemolyticus. J. Diarrhoeal Dis. Rev., 13:219-223, 1995
  36. Zhang, X.-H. and Austin, B.: Pathogenicity of Vibrio harveyi to salmonids. J. Fish Dis., 23:93-102, 2000 https://doi.org/10.1046/j.1365-2761.2000.00214.x
  37. 권문경, 박상언, 방종득, 박수일: 넙치, Paralichthys olivaceus에서 병원성 Photobacterium damselae subsp. damselae의 분리. 한국어병학회지, 18:205-214, 2005