The Korean Journal of Pain

  • 제22권1호
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  • Pages.21-27
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  • 2009
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  • 2005-9159(pISSN)
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  • 2093-0569(eISSN)
대한통증학회 (The Korean Pain Society)
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백서의 척수신경결찰모델에서 Morphine의 투여가 항이질통 효과와 척수 α2 아드레날린계 수용체 아형 mRNA 발현에 미치는 영향

The Antiallodynic Effect and the Change of the α2 Adrenergic Receptor Subtype mRNA Expression by Morphine Administration in a Spinal Nerve Ligation Rat Model

  • 정규연 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 신상욱 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 권수아 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 김태균 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 백승훈 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 백승완 (부산대학교 의학전문대학원 마취통증의학교실)
  • Chung, Kyu Yeon (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Shin, Sang Wook (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Kwon, Su Ah (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Kim, Tae Kyun (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Baek, Seung Hoon (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Baik, Seong Wan (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University)
  • 투고 : 2009.02.03
  • 심사 : 2009.03.25
  • 발행 : 2009.04.01
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초록

Background: The neuropathic pain arising from nerve injury is difficult to treat and the therapeutic effects of opioid drugs remain debatable. Agonists acting at the ${\alpha}_2$ adrenergic and opioid receptors have analgesic properties and they act synergistically when co-administered in the spinal cord. The lack of subtype-selective pharmacological agents has previously impeded the synergistic effects that are mediated by the adrenergic receptor subtypes. Methods: We created neuropathic pain model by ligating the L5 spinal nerve in Sprague-Dawley rats (n = 18). We divided the rats into three groups (n = 6 for each group), and we administered intraperitoneal morphine (1 mg/kg, 3 mg/kg, 5 mg/kg) and then we measured the mechanical allodynia with using von-Frey filaments for 8 hours. We then injected morphine (5 mg/kg) intraperitoneally, twice a day for 2 weeks. We measured the tactile and cold allodynia in the morphine group (n = 9) and the saline group (n = 9). After 2 weeks, we decapitated the rats and harvested the spinal cords at the level of lumbar enlargement. We compared the ${\alpha}_2$ subtype mRNA expression with that of control group (n = 6) by performing real time polymerase chain reaction (RTPCR). Results: Intraperitoneal morphine reduced the neuropathic pain behavior in the dose-dependent manner. Chronic morphine administration showed an antiallodynic effect on the neuropathic pain rat model. The rats did not display tolerance or hyperalgesia. The expression of the mRNAs of the ${\alpha}_{2A}$, ${\alpha}_{2B}$, ${\alpha}_{2C}$ subtypes decreased, and morphine attenuated this effect. But we could not get statistically proven results. Conclusions: Systemic administration of morphine can attenuate allodynia during both the short-term and long-term time course. Morphine has an influence on the expression of ${\alpha}_2$ receptor subtype mRNA. Yet we need more research to determine the precise effect of morphine on the ${\alpha}_2$ subtype gene expression.

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과제정보

연구 과제 주관 기관 : 부산대학교

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