Archives of Plastic Surgery

  • 제36권2호
  • /
  • Pages.135-139
  • /
  • 2009
  • /
  • 2234-6163(pISSN)
  • /
  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
권호 표지

1년 이상 냉동 보관한 흡인 지방조직 내의 세포 생존

Viability of cells in aspirated fat tissue after 1 year cryopreservation

  • 손대구 (계명대학교 의과대학 성형외과학교실) ;
  • 오재훈 (가가성형외과) ;
  • 최태현 (계명대학교 의과대학 성형외과학교실) ;
  • 김준형 (계명대학교 의과대학 성형외과학교실) ;
  • 한기환 (계명대학교 의과대학 성형외과학교실)
  • Son, Daegu (Department of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center) ;
  • Oh, Jaehoon (Gaga Aesthetic Plastic Surgical Clinic) ;
  • Choi, Taehyun (Department of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center) ;
  • Kim, Junhyung (Department of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center) ;
  • Han, Kihwan (Department of Plastic and Reconstructive Surgery, Keimyung University Dongsan Medical Center)
  • 발행 : 2009.03.15
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose: The use of an autogenous fat graft has become a common procedure in plastic surgery. However, questions remain concerning on the viability of fat cells and preservation method of aspirated fat. The purpose of this study was to examine the viability of fat cells stored at $-20^{\circ}C$ in the freeze for 1 year after harvest from abdominal liposuction. Methods: Eighteen adults (aged 24 to 65 years old, 16 female and 2 male) were recruited for this study. Harvested aspirated fat tissues were obtained by suction - assisted lipectomy and frozen at $-20^{\circ}C$ commercial refrigerator for one year (average 12.5 months). The viability off at cells in specimens were measured after thawing. The numbers of viable cells were measured on a fluorescence microscope after staining with fluorescein diacetate and propidium iodide. GPDH (Glycerol - 3 - phosphate dehydrogenase) activity was measured. Cell culture was done for 3 weeks. Results: There were no viable cells under the fluorescence microscope, no detectable GPDH activity, and no cultured cells. Conclusion: These findings suggest that aspirated fat after frozen storage for one year at $-20^{\circ}C$ freezer is inadequate to reuse.

키워드

참고문헌

  1. Scarborough DA, Schuen W, Bisaccia E: Fat transfer for aging skin: technique for rhytids. J Dermatol Surg Oncol 16: 651, 1990 https://doi.org/10.1111/j.1524-4725.1990.tb00095.x
  2. Pereira LH, Radwanski HN: Fat grafting of the buttocks and lower limbs. Aesthetic Plastic Surg 20: 409, 1996 https://doi.org/10.1007/BF02390316
  3. Guerrerosantos J, Gonzalez-Mendoza A, Masmela Y, Gonzalez MA, Deos M, Diaz P: Long-term survival of free fat grafts in muscle: an experimental study in rats. Aesthetic Plast Surg 20: 403, 1996 https://doi.org/10.1007/BF02390315
  4. Moore JH Jr, Kolaczynski JW, Morales LM, Considine RV, Pietrzkowski Z, Noto PF, Caro JF: Viability of fat obtained by syringe suction lipectomy: effects of local anesthesia with lidocaine. Aesthetic Plast Surg 19: 335, 1995 https://doi.org/10.1007/BF00451659
  5. Shiffman MA, Mirrafati S: Fat transfer techniques: The effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg 27: 819, 2001 https://doi.org/10.1046/j.1524-4725.2001.01062.x
  6. Pu LL, Cui X, Fink BF, Cibull ML, Gao D: The viability of fatty tissues within adipose aspirates after conventional liposuction: a comprehensive study. Ann Plast Surg 54: 288, 2005
  7. Schuller-Petrovic S: Improving the aesthetic aspect of soft tissue defects on the face using autologous fat transplantation. Facial Plast Surg 13: 119, 1997 https://doi.org/10.1055/s-2008-1064474
  8. Sommer B, Sattler G: Current concept of fat graft survival: histology of aspirated adipose tissue and review of the literature. Dermatol Surg 26: 1159, 2000 https://doi.org/10.1046/j.1524-4725.2000.00278.x
  9. Wolter TP, von Heimburg D, Stoffels I, Groeger A, Pallua N: Cryopreservation of mature human adipocytes: in vitro measurement of viability. Ann Plast Surg 55: 408, 2005 https://doi.org/10.1097/01.sap.0000181345.56084.7d
  10. Liu J, Woods EJ, Agca Y, Critser ES, Critser JK: Cryobiology of rat embryos II: a theoretical model for the development of interrupted slow freezing procedure. Biol Reprod 63: 1303, 2000 https://doi.org/10.1095/biolreprod63.5.1303
  11. Tchoukalova YD, Harteneck DA, Karwoski RA, Tarara J, Jensen MD: A quick, reliable, and automated method for fat cell sizing. J Lipid Res 44: 1795, 2003 https://doi.org/10.1194/jlr.D300001-JLR200
  12. von Heimburg D, Zachariah S, Heschel I, Kühling H, Schoof H, Hafemann B, Pallua N: Human preadipocytes seeded on freeze-dried collagen scaffolds investigated in vitro and in vivo. Biomaterials 5: 429, 2001
  13. Rohrich RJ, Sorokin ES, Brown SA: In search of imporved fat transfer viability: a quantitative analysis of the role of centrifugation and harvest site. Plast Reconstr Surg 113: 391, 2004 https://doi.org/10.1097/01.PRS.0000097293.56504.00
  14. Matsumoto D, Shigeura T, Sato K, Inoue K, Suga H, Kato H, Aoi N, Murase S, Gonda K, Yoshimura K: Influence of preservation at various temperatures on liposuction aspirates. Plast Reconstr Surg 120: 1510, 2007 https://doi.org/10.1097/01.prs.0000288015.70922.e4
  15. MacRae JW, Tholpady SS, Ogle RC, Morgan RF: Ex vivo fat graft preservation; Effects and implications of cryopreservation. Ann Plast Surg 52: 281, 2004 https://doi.org/10.1097/01.sap.0000110559.58945.2e