Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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H19 Gene Is Epigenetically Stable in Mouse Multipotent Germline Stem Cells

  • Oh, Shin Hye (Department of Bioscience and Biotechnology, Bio-Organ Research Center, Konkuk University) ;
  • Jung, Yoon Hee (Department of Bioscience and Biotechnology, Bio-Organ Research Center, Konkuk University) ;
  • Gupta, Mukesh Kumar (Department of Bioscience and Biotechnology, Bio-Organ Research Center, Konkuk University) ;
  • Uhm, Sang Jun (Department of Bioscience and Biotechnology, Bio-Organ Research Center, Konkuk University) ;
  • Lee, Hoon Taek (Department of Bioscience and Biotechnology, Bio-Organ Research Center, Konkuk University)
  • Received : 2008.12.10
  • Accepted : 2009.04.21
  • Published : 2009.06.30
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Abstract

Testis-derived germline stem (GS) cells can undergo reprogramming to acquire multipotency when cultured under appropriate culture conditions. These multipotent GS (mGS) cells have been known to differ from GS cells in their DNA methylation pattern. In this study, we examined the DNA methylation status of the H19 imprinting control region (ICR) in multipotent adult germline stem (maGS) cells to elucidate how epigenetic imprints are altered by culture conditions. DNA methylation was analyzed by bisulfite sequencing PCR of established maGS cells cultured in the presence of glial cell line-derived neurotrophic factor (GDNF) alone or both GDNF and leukemia inhibitory factor (LIF). The results showed that the H19 ICR in maGS cells of both groups was hypermethylated and had an androgenetic pattern similar to that of GS cells. In line with these data, the relative abundance of the Igf2 mRNA transcript was two-fold higher and that of H19 was three fold lower than in control embryonic stem cells. The androgenetic DNA methylation pattern of the H19 ICR was maintained even after 54 passages. Furthermore, differentiating maGS cells from retinoic acid-treated embryoid bodies maintained the androgenetic imprinting pattern of the H19 ICR. Taken together these data suggest that our maGS cells are epigenetically stable for the H19 gene during in vitro modifications. Further studies on the epigenetic regulation and chromatin structure of maGS cells are therefore necessary before their full potential can be utilized in regenerative medicine.

Keywords

Acknowledgement

Supported by : Rural Development Administration

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