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This study intends to evaluate the efficiency of non-point source reduction technique by using the porous lightweight and recycled aggregate which microorganism is seeded. In case of infiltration velocity 30~70 mm/hr in high concentration of influent, it is indicated that SS was 40~94%, COD 44~91%, BOD 4~91%, TN 1.2~66%, TP 7~70% of removal efficiency. Removal efficiency is good in infiltration velocity 30 > 50 > 70㎜/hr order. Therefore, the non-point source treatment facility filled with lightweight and recycled aggregate using microbial seeding shows higher removal efficiency than a conventional sand and gravel. We confirm that the function and efficiency are improved significantly and applied to treat non-point sources.