Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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The efficacy and influence on growth of olive flounder Paralichthys olivaceus vaccinated against Edwardsiella tarda and Streptococcus iniae

넙치, Paralichthys olivaceus에 투여된 Edwardsiella tarda와 Streptococcus iniae에 대한 혼합백신의 예방효과와 성장에 미치는 영향

  • Kim, Myoung-Sug (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Kyoung-Duck (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Kim, Kang-Woong (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Jin-Woo (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute)
  • 김명석 (국립수산과학원 병리연구과) ;
  • 김경덕 (국립수산과학원 양식관리과) ;
  • 김강웅 (국립수산과학원 양식관리과) ;
  • 박명애 (국립수산과학원 병리연구과) ;
  • 김진우 (국립수산과학원 수산동물방역센타)
  • Published : 2009.12.01
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Abstract

This study was performed to verify the efficacy and safety of vaccine mixed formalin killed Edwardsiella tarda and formalin killed Streptococcus iniae in olive flounder Paralichthys olivaceus. Sera were obtained from each group at 2, 4, 6 and 8 weeks after vaccination and agglutination titers to E. tarda and S. iniae were determined using the microtiter method. Three weeks after vaccination, the fish challenged by intraperitoneal injection of E. tarda or S. iniae and immunization resulted in a relative percentage survival (RPS) of above 81.0-92.8%. Neither the groups vaccinated nor control groups resulted in significantly reduced weight gain 140 days post-vaccination, but differences between the groups vaccinated and control groups were found at the early days post-vaccination. This results suggest that it is possible to prevent edwardsiellosis and streptococosis after a vaccination without affecting growth of olive flounder.

본 연구는 넙치, Paralichthys olivaceus에서 Edwardsiella tarda와 Streptococcus iniae에 대한 혼합백신의 효능과 안전성에 대해 조사하고자 하였다. 혼합백신을 주사한 넙치에서 2, 4, 6, 8주 째에 채혈하여 E. tarda와 S. iniae에 대한 항체가 를 microtitre 법으로 조사하였다. 백신 주사 후 3주째에 E. tarda와 S. iniae를 각각 인위감염 시켰을 때 백신구에서 81.0-92.8%의 높은 상대생존율을 보여 혼합백신에 의한 항병력의 증가가 확인되었다. 백신 주사 후 초기에 백신구가 대조구에 비해 체중 증가가 느렸으나 사육기간이 길어지면서 회복되어 대조구와 백신구 체중의 차이를 보이지 않았다. 이 결과는 백신 주사가 넙치의 성장에 영향을 주지 않고 에드와드병과 연쇄구균증을 예방할 수 있음을 보여준다.

Keywords

References

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