Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer

  • Zhang, Y.H. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Song, E.S. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim, E.S. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Cong, P.Q. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Lee, S.H. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Lee, J.W. (Department of Animal Resource Science, College of Industrial Science, Kongju National University) ;
  • Yi, Y.J. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Park, Chang-Sik (Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • Received : 2008.12.16
  • Accepted : 2009.03.19
  • Published : 2009.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) removal on the development of nuclear transfer (NT) embryos. There were no significant differences in the rates of fusion and cleavage among the five different oviductal fluid concentrations. However, the rates of blastocyst formation and the cell numbers per blastocyst were high in the embryos at the 14 and 28 $\mu{g}$/ml concentrations of oviductal fluid compared to the 0, 56 and 100 $\mu{g}$/ml concentrations. The rates of cleavage and blastocyst formation, and the cell numbers per blastocyst were higher in the PZM-3, PZM-5 and modified PZM-5 media than in the PZM-4 medium. However, there were no significant differences in the fusion rates of oocytes among the four culture media. The cell numbers per blastocyst in the embryos without ZP were significantly greater than those with ZP. However, there were no significant differences in the rates of fusion, cleavage and blastocyst formation between the embryos with and without ZP. In conclusion, we improved blastocyst development and the quality of NT embryos by replacing PVA with 3 mg/ml of BSA in PZM-5 medium and supplementing the PZM-5 medium with 14 $\mu{g}$/ml oviductal fluid. The NT embryos produced by the zona-free NT method had a high rate of blastocyst formation in the modified PZM-5 medium.

Keywords

References

  1. Abe, H. and T. Oikawa. 1990. Ultrastructural evidence for an association between an oviductal glycoprotein on the zona pellucida of the golden hamster egg. J. Exp. Zool. 256:210-221
  2. Abe, H., C. Numazawa, M. Abe, H. Hoshi and T. Oikawa. 1993. Production and characterization of monoclonal antibodies specific for bovine oviductal glycoproteins. J. Exp. Zool. 265:77-83 https://doi.org/10.1002/jez.1402650111
  3. Archibong, A. E., R. M. Petters and B. H. Johnson. 1989. Development of porcine embryos from one- and two-cell stages to blastocysts in culture medium supplemented with porcine oviductal fluid. Biol. Reprod. 41(6):1076-1083 https://doi.org/10.1095/biolreprod41.6.1076
  4. Bavister, B. D. 1995. Culture of preimplantation embryos: facts and artifacts. Hum. Reprod. Update 1(2):91-148 https://doi.org/10.1093/humupd/1.2.91
  5. Biggers, J. D., J. A. Lawitts and C. P. Lechene. 1993. The protective action of betine on the deleterious effects of NaCl on preimplantation mouse embryos in vitro. Mol. Reprod. Dev. 34:380-390 https://doi.org/10.1002/mrd.1080340406
  6. Boice, M. L., R. D. Geisert, R. M. Blair and M. G. Verhage. 1990. Identification and characterization of bovine oviductal glycoproteins synthesized at estrus. Biol. Reprod. 43:457-465 https://doi.org/10.1095/biolreprod43.3.457
  7. Booth, P. J., S. J. Tan, R. Reipurth, P. Holm and H. Callesen. 2001a. Simplification of bovine somatic cell nuclear transfer by application of a zona-free manipulation technique. Cloning Stem Cells 3(3):139-150 https://doi.org/10.1089/153623001753205098
  8. Booth, P. J., S. J. Tan, P. Holm and H. Callesen. 2001b. Application of the zona-free manipulation technique to porcine somatic nuclear transfer. Cloning Stem Cells 3(3):191-197
  9. Brown, C. R. and W. K. Cheng. 1986. Changes in composition of the porcine zona pellucida during development of the oocyte to the 2- to 4-cell embryo. J. Embryol. Exp. Morphol. 92:183-191
  10. Buhi, W. C., J. L. Vallet and F. W. Bazer. 1989. De novo synthesis and release of polypeptides from cyclic and early pregnant porcine oviductal tissue in explant culture. J. Exp. Zool. 252:79-88 https://doi.org/10.1002/jez.1402520111
  11. Dawson, K. M. and J. M. Baltz. 1997. Organic osmolytes and embryos: substrates of the Gly andβ transport systems protect mouse zygotes against the effects of raised osmolarity. Biol. Reprod. 56:1550-1558 https://doi.org/10.1095/biolreprod56.6.1550
  12. Galli, C., I. Lagutina, G. Crotti, S. Colleoni, P. Turini, N. Ponderato, R. Duchi and G. Lazzari. 2003. Pregnancy a cloned horse born to its dam twin. Nature 424:635 https://doi.org/10.1038/424635a
  13. Gandolfi, F., S. Modina, T. A. Brevini, C. Galli, R. M. Moor and A. Lauria. 1991. Oviduct ampullary epithelium contributes a glycoprotein to the zona pellucida, perivitelline space and blastomere membranes of sheep embryos. Eur. J. Basic Appl. Histochem. 35:383-392 https://doi.org/10.1095/biolreprod.102.011874
  14. Hammer, M. A. and J. M. Baltz. 2003. $\beta$-Alanine but not taurine can function as an organic osmolyte in preimplantation mouse embryos cultured from fertilized eggs. Mol. Reprod. Dev. 66:153-161 https://doi.org/10.1002/mrd.10343
  15. Hedrick, J. L., J. J. Wardrip and T. Berger. 1987. Differences in the macromolecular composition of the zona pellucida isolated from pig oocytes, eggs, and zygotes. J. Exp. Zool. 241:257-262 https://doi.org/10.1002/jez.1402410213
  16. Im, G. S., L. Lai, Z. Liu, Y. Hao, D. Wax, A. Bonk and R. S. Prather. 2004. In vitro development of preimplanation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Theriogenol. 61:1125-1135 https://doi.org/10.1016/j.theriogenology.2003.06.006
  17. Kikuchi, K., N. Kashiwazaki, J. Noguchi, A. Shimada, R. Takahashi, M. Hirabayashi, M. Shino, M. Ueda and H. Kaneko. 1999. Developmental competence, after transfer to recipients, of porcine matured, fertilized, and cultured in vitro. Biol. Repord. 60:336-340 https://doi.org/10.1095/biolreprod60.2.336
  18. Kikuchi, K., A. Onishi, N. Kashiwazaki, M. Iwamoto, J. Noguchi, H. Kaneko, T. Akita and T. Nagai. 2002. Successful piglet production after transfer of blastocysts produced by a modified in vitro system. Biol. Repord. 66:1033-1041 https://doi.org/10.1095/biolreprod66.4.1033
  19. Krisher, R. L., R. M. Petters, B. H. Johnson, B. D. Bavister and A. E. Archibong. 1989. Development of porcine embryos from the one-cell stage to blastocyst in mouse oviducts maintained in organ culture. J. Exp. Zool. 249(2):235-239 https://doi.org/10.1002/jez.1402490217
  20. Lagutina, I., G. Lazzari, R. Duchi, S. Colleoni, N. Ponderato, P. Turini, G. Crotti and C. Galli. 2005. Somatic cell nuclear transfer in horses: effect of oocyte morphology, embryo reconstruction method and donor cell type. Reproduction 130:559-567 https://doi.org/10.1530/rep.1.00772
  21. Lagutina, I., G. Lazzari and C. Galli. 2006. Birth of cloned pigs from zona-free nuclear transfer blastocysts developmed in vitro before transfer. Cloning Stem Cells 8(4):283-293 https://doi.org/10.1089/clo.2006.8.283
  22. Leese, H. J. 1988. The formation and function of the oviduct fluid. J. Reprod. Fertil. 82:843-856 https://doi.org/10.1016/S0034-5288(98)90154-0
  23. Li, R. F., L. H. Wen, S. Y. Wang and S. Bou. 2003. Free amino acid content of oviductal and uterine fluid at different oestrous stages in the cow. Acta. Zoologica. Sinica. 49:80-85
  24. Minami, N., B. D. Bavister and A. Iritani. 1988. Development of hamster two-cell embryos in the isolated mouse oviduct in organ culture system. Gamete Res. 19(3):235-240 https://doi.org/10.1002/mrd.1120190303
  25. Oback, B., A. T. Wiersema, P. Gaynor, G. Laible, F. C. Tucker, J. E. Oliver, A. L. Miller, H. E. Troskie, K. L. Wilson, J. T. Forsyth, M. C. Berg, K. Cockrem, V. McMillan, H. R. Tervit and D. N. Wells. 2003. Cloned cattle derived from a novel zona-free embryo reconstruction system. Cloning Stem Cells 5:3-12 https://doi.org/10.1089/153623003321512111
  26. Parrish, J. J., J. L. Susko-Parrish, R. R. Handrow, M. M. Sims and N. L. First. 1989. Capacitation of bovine spermatozoa by oviduct fluid. Biol. Reprod. 40(5):1020-1025 https://doi.org/10.1095/biolreprod40.5.1020
  27. Pemble, L. B. and P. L. Kaye. 1986. Whole protein uptake and metabolism by mouse blastocysts. J. Reprod. Fertil. 78(1):149-157 https://doi.org/10.1530/jrf.0.0780149
  28. Petters, R. M. and M. L. Reed. 1991. Addition of taurine or hypotaurine to culture medium improves development of one and two-cell pig embryos in vitro. Theriogenology 35:253 https://doi.org/10.1016/0093-691X(91)90229-7
  29. Petters, R. M. and K. D. Wells. 1993. Culture of pig embryos. J. Repord. Fert. Suppl. 48:61-73
  30. Peura, T. T., I. M. Lewis and A. O. Trounson. 1998. The effect of recipient oocyte volume on nuclear transfer in cattle. Mol. Reprod. Dev. 50(2):185-191 https://doi.org/10.1002/(SICI)1098-2795(199806)50:2<185::AID-MRD9>3.0.CO;2-G
  31. Quinn, P., J. F. Kerin and G. M. Warnes. 1985. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil. Steril. 44(4):493-498 https://doi.org/10.1385/1-59259-340-2:35
  32. Rexroad, C. E. Jr. and A. M. Powell. 1988. Co-culture of ovine ova with oviductal cells in medium 199. J. Anim. Sci. 66(4):947-953 https://doi.org/10.1016/0093-691X(89)90568-2
  33. Roberts, G. P., J. M. Parker and M. W. Symonds. 1975. Proteins in the luminal fluid from the bovine oviduct. J. Reprod. Fertil. 45:301-313 https://doi.org/10.1530/jrf.0.0450301
  34. Sendai, Y., H. Abe, M. Kikuchi, T. Satoh and H. Hoshi. 1994. Purification and molecular cloning of bovine oviduct-specific glycoprotein. Biol. Reprod. 50:835-844 https://doi.org/10.1095/biolreprod50.4.835
  35. Suzuki, C., K. Yoshioka and S. Iwamura. 2002. Effects of amino acids and BSA on the development of in vitro produced porcine embryos. Programs of the 95th Annual Meeting of the Japanese Society of Animal Reproduction, Iwate, Japan
  36. Suzuki, C., S. Iwamura and K. Yoshioka. 2004. Birth of piglets through the non-surgical transfer of blastocysts produced in vitro. J. Reprod. Dev. 50(4):487-491 https://doi.org/10.1262/jrd.50.487
  37. Tervit, H. R., D. G. Whittingham and L. E. Rowson. 1972. Successful culture in vitro of sheep and cattle ova. J. Reprod. Fertil. 30(3):493-497 https://doi.org/10.1016/S0093-691X(97)00134-9
  38. Vajta, G., I. M. Lewis, P. Hyttel, G. A. Thouas and A. O. Trounson. 2001. Somatic cell cloning without micromanipulators. Cloning 3(2):89-95 https://doi.org/10.1089/15204550152475590
  39. Vajta, G., P. Bartels, J. Joubert, M. de la Rey, R. Treadwell and H. Callesen. 2004. Production of a healthy calf by somatic cell nuclear transfer without micromanipulators and carbon dioxide incubators using the handmade cloning (HMC) and the submarine incubation system (SIS). Theriogenology 62:1465-1472 https://doi.org/10.1016/j.theriogenology.2004.02.010
  40. Vajta, G., Y. Zhang and Z. Machaty. 2007. Somatic cell nuclear transfer in pigs: recent achievements and future possibilities. Reprod. Fertil. Dev. 19:403-423 https://doi.org/10.1071/RD06089
  41. Vierbuchen, M. 1991. Lectin Receptors. In: Current topics in pathology (Ed. C. L. Berry and E. Grundmann). 83:271-361
  42. Wegner, C. C. and G. J. Killian. 1991. In vitro and in vivo association of an oviduct estrus-associated protein with bovine zona pellucida. Mol. Reprod. Dev. 29:77-84 https://doi.org/10.1002/mrd.1080290112
  43. Wu, G. M., L. Lai, J. Mao, T. C. McCauley, J. N. Caamaño, T. Cantley, A. Rieke, C. N. Murphy, R. S. Prather, B. A. Didion and B. N. Day. 2004. Birth of piglets by in vitro fertilization of zona-free porcine oocytes. Theriogenology 62:1544-1566 https://doi.org/10.1016/j.theriogenology.2004.02.016
  44. Yoshioka, K., C. Suzuki, A. Tanaka, M. K. Anas Idris and S. Iwamura. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 66:112-119 https://doi.org/10.1095/biolreprod66.1.112
  45. Zhang, Y. H., E. S. Song, E. S. Kim, P. Q. Cong, S. H. Lee, J. W. Lee, Y. J. Yi and C. S. Park. 2009. Effects of donor cell passage, size and type on development of porcine embryos derived from somatic cell nuclear transfer. Asian-Aust. J. Anim. 22:194-200