Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Molecular identification and expression analysis of a natural killer enhancing factor-A from black rockfish Sebastes schlegelii

  • Lee, Jeong-Ho (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Joo-Won (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Park, Chan-Il (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
  • Published : 2009.12.01
⟨ Previous Next ⟩

Abstract

Natural-killer-cell-enhancing factor (NKEF) belongs to the newly defined peroxiredoxin (Prx) family. It was originally isolated from human erythroid cells. The black rockfish NKEF cDNA was identified through the expressed sequence tag (EST) analysis of PBLs libraries. The full-length NKEF cDNA was 1433 bp long and contained an open reading frame (ORF) of 594 bp that encoded 198 amino-acid residues. The 5' UTR had a length of 39 bp, and the 3’UTR 800 bp. The deduced amino-acid sequence of the black rockfish had a density 93.4, 92.9, 87.8, 85.8, 84.8, 83.8, 80.3, 79.7, 77.2, and 75.2% that of the pufferfish, olive flounder, channel catfish, zebrafish, chicken, common carp, Myotis lucifugus, cattle, human PrxI, rat PrxI, human NKEF-A, and Xenopus tropicalis, respectively. The NKEF gene was expressed in all the tissues of the black rockfish. The RT-PCR indicated that the NKEF transcripts were predominantly in the spleen and gill, less dominantly in the PBLs, head kidney, trunk kidney, and liver, and least in the intestine and muscles. This is the first report on the existence of the NKEF-A gene in black rockfish.

Keywords

References

  1. Baeck, G.W., Kim, J.W., Kim, K.H., Jun, K.Y., An, G.H. and Park, C.I.: Expressed Sequence Tags Analysis of Black Rockfish (Sebastes schlegelii) Peripheral Leukocytes Stimulated with Con A/PMA or LPS. J Fish Pathol., 21:129-137, 2008
  2. Baeck, G.W., Kim, J.W. and Park, C.I.: Identification and expression analysis of an interferon stimulated gene 15 (ISG15) from black rockfish, Sebastes schlegelii. Fish Shellfish Immunol., 25:679-81, 2008 https://doi.org/10.1016/j.fsi.2008.08.005
  3. Chae, H.Z., Robison, K., Poole, L.B., Church, G., Storz, G. and Rhee, S.G.: Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol specific antioxidant define a large family of antioxidant enzymes. Proc. Natl. Acad. Sci. U.S.A. 91:7017-7021, 1994 https://doi.org/10.1073/pnas.91.15.7017
  4. Chen, Y., Zhang, Y.X., F, T.J., Meng, L., Ren, G.C. and Chen, S.L.: Molecular identification and expression analysis of the natural killer cell enhancing factor (NKEF) gene from turbot (Scophthalmus maximus) Aquaculture, 261:1186-1193, 2006 https://doi.org/10.1016/j.aquaculture.2006.09.034
  5. Dong, W.R., Xiang, L.X. and Shao, J.Z.: Cloning and characterisation of two natural killer enhancing factor genes (NKEF-A and NKEF-B) in pufferfish, Tetraodon nigroviridis. Fish Shellfish Immunol., 22:1-15, 2007 https://doi.org/10.1016/j.fsi.2006.03.007
  6. Geiben-Lynn, R., Kursar, M., Brown, N.V., Addo, M.M., Shau, H. and Lieberman, J.: HIV-1 antiviral activity of recombinant natural killer cell enhancing factors, NKEF-A and NKEF-B, members of the peroxiredoxin family. J Biol Chem., 278:1569-1574, 2003 https://doi.org/10.1074/jbc.M209964200
  7. Gish, W. and David, J.S.: Identification of protein coding regions by database similarity search. Nature Genetics, 3:266-272, 1993 https://doi.org/10.1038/ng0393-266
  8. Herberman, R.B., Nunn, M.E., Holden, H.T. and Lavrin, D.H.: Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells. Int J Cancer, 16:230-239, 1975 https://doi.org/10.1002/ijc.2910160205
  9. Herberman, R.B. and Ortaldo, JR.: Natural killer cells: their roles in defenses against disease. Science, 214:24-30, 1981 https://doi.org/10.1126/science.7025208
  10. Jin, D.Y., Chae, H.Z., Rhee, S.G. and Jeang, K.T.: Regulatory role for a novel human thioredoxin peroxidase in NF-RB activation. J. Biol. Chem., 272:30952-30961, 1997 https://doi.org/10.1074/jbc.272.49.30952
  11. Kim, A.T., Sarafian, T.A. and Shau, H.: Characterization of antioxidant properties of natural killer-enhancing factor-B and induction of its expression by hydrogen peroxide. Toxicology and Applied Pharmacology, 147:135-142, 1997 https://doi.org/10.1006/taap.1997.8270
  12. Kim, H., Lee, T.H., Park, E.S., Suh, J.M. and Park, S.J., Chung, H.K.: Role of peroxiredoxins in regulating intracellular hydrogen peroxide and hydrogen peroxide-induced apoptosis in thyroid cells. J Biol Chem., 275:18266-18270, 2000 https://doi.org/10.1074/jbc.M001763200
  13. Kuznetsov, V.A.: "Harpoon"model for cell-cell adhesion and recognition of target cells by the natural killer cells. Journal of Theoretical Biology, 180:321-342, 1996 https://doi.org/10.1006/jtbi.1996.0106
  14. Li, R.W. and Waldbieser, G.C.: Genomic organisation and expression of the natural killer cell enhancing factor (NKEF) gene in channel catfish Ictalurus punctatus(Rafinesque). Fish Shellfish Immunol., 20:72-82, 2006 https://doi.org/10.1016/j.fsi.2005.04.002
  15. Prosperi, M.T., Ferbus, D., Karczinski, I. and Goubin, G.: A human cDNA corresponding to a gene overexpressed during cell proliferation encodes a product sharing homology with amoebic and bacterial proteins. J Biol Chem., 268:11050-11056, 1993
  16. Sato, H. and Bannai, S.: Peroxiredoxin: a new host antioxidation system. Seikagaku 71:333-337, 1999
  17. Sauri, H., Butterfield, L., Kim, A. and Shau, H.: Antioxidant function of recombinant human natural killer enhancing factor. Biochemical and Biophysical Research Communications, 208:964-969, 1995 https://doi.org/10.1006/bbrc.1995.1428
  18. Saur,i H., Ashjian, P.H., Kim, A.T. and Shau, H.: Recombinant natural killer enhancing factor augments natural killer cytotoxicity. J Leukoc Biol., 59:925-931, 1996
  19. Schroder, E., Willis, A.C. and Ponting, C.P.: Porcine natural killer enhancing factor-B: oligomerisation and identification as a calpain substrate in vitro. Biochim Biophys Acta., 1383:279-291, 1998 https://doi.org/10.1016/S0167-4838(97)00217-3
  20. Shau, H., Gupta, R.K. and Golub, S.H.: Identification of natural killer enhancing factor (NKEF) from human erythroid cells. Cell. Immunol., 147:1-11, 1993 https://doi.org/10.1006/cimm.1993.1043
  21. Shau, H., Butterfield, L.H., Chiu, R. and Kim, A.: Cloning and sequence analysis of candidate human natural killer enhancing factor genes. Immunogenetics, 40:129-134, 1994 https://doi.org/10.1007/BF00188176
  22. Shau, H. and Kim, A.: Identification of natural killer enhancing factor as a major antioxidant in human red blood cells. Biochem Biophys Res Commun., 199:83-88, 1994 https://doi.org/10.1006/bbrc.1994.1197
  23. Shen, L., Stuge, T.B., Zhou, H., Khayat, M., Barker, K,S. and Quiniou, S.M.: Channel catfish cytotoxic cells: a mini-review. Dev Comp Immunol., 26:141-149, 2002 https://doi.org/10.1016/S0145-305X(01)00056-8
  24. Shin, D.H., Fujiki, K., Nakao, M. and Yano, T.: Organization of the NKEF gene and its expression in the common carp (Cyprinus carpio). Dev. Comp. Immunol., 25:597-606, 2001 https://doi.org/10.1016/S0145-305X(01)00021-0
  25. Thompson, J.D., Higgis, D.G. and Gibson, T.J.: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res., 22:4673-4680, 1994 https://doi.org/10.1093/nar/22.22.4673
  26. Wang, Z., Chen, S.L. and Ji, X.S.: Cloning and expression analysis of natural killer enhancing factor (NKEF) from Japanese flounder (Paralichthys olivaceus). Chin. High Technol. Lett., 15:86-90, 2005
  27. Wood, Z.A., Schrode,r E., Robin-Harris, J. and Poole, L.B.: Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci., 28:32-40, 2003 https://doi.org/10.1016/S0968-0004(02)00003-8
  28. Yamamoto, T., Matsui, Y., Natori, S. and Obinata, M.: Cloning of a housekeeping-type gene (MER5) preferentially expressed in murine erythroleukemia cells. Gene, 80:337-343, 1989 https://doi.org/10.1016/0378-1119(89)90297-7
  29. Zhang, P., Liu, B., Kang, S.W., Seo, M.S., Rhee, S.G. and Obeid, L.M.: Thioredoxin peroxidase is a novel inhibitor of apoptosis with a mechanism distinct from that of Bcl-2. J Biol Chem., 272:30615-30618, 1997 https://doi.org/10.1074/jbc.272.49.30615
  30. Zhang, H., Evenhuis, J.P., Thorgaard, G.H. and Ristow, S.S.: Cloning, characterization and genomic structure of the natural killer cell enhancement factor (NKEF)-like gene from homozygous clones of rainbow trout. Dev. Comp. Immunol., 25:25-35, 2001 https://doi.org/10.1016/S0145-305X(00)00038-0