Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Hepatitis Delta Virus Large Antigen Sensitizes to TNF-α-Induced NF-κB Signaling

  • Park, Chul-Yong (Ilsong Institute of Life Science, Hallym University) ;
  • Oh, Sang-Heun (Ilsong Institute of Life Science, Hallym University) ;
  • Kang, Sang Min (Ilsong Institute of Life Science, Hallym University) ;
  • Lim, Yun-Sook (Ilsong Institute of Life Science, Hallym University) ;
  • Hwang, Soon B. (Ilsong Institute of Life Science, Hallym University)
  • Received : 2009.05.06
  • Accepted : 2009.05.22
  • Published : 2009.07.31
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Abstract

Hepatitis delta virus (HDV) infection causes fulminant hepatitis and liver cirrhosis. To elucidate the molecular mechanism of HDV pathogenesis, we examined the effects of HDV viral proteins, the small hepatitis delta antigen (SHDAg) and the large hepatitis delta antigen (LHDAg), on $NF-{\kappa}B$ signaling pathway. In this study, we demonstrated that $TNF-{\alpha}-induced$ $NF-{\kappa}B$ transcriptional activation was increased by LHDAg but not by SHDAg in both HEK293 and Huh7 cells. Furthermore, LHDAg promoted TRAF2-induced $NF-{\kappa}B$ activation. Using coimmunoprecipitation assays, we demonstrated that both SHDAg and LHDAg interacted with TRAF2 protein. We showed that isoprenylation of LHDAg was not required for the increase of $NF-{\kappa}B$ activity. We further showed that only LHDAg but not SHDAg increased the $TNF-{\alpha}-mediated$ nuclear translocation of p65. This was accomplished by activation of $I{\kappa}B_{\alpha}$ degradation by LHDAg. Finally, we demonstrated that LHDAg augmented the COX-2 expression level in Huh7 cells. These data suggest that LHDAg modulates $NF-{\kappa}B$ signaling pathway and may contribute to HDV pathogenesis.

Keywords

Acknowledgement

Supported by : Ministry of Health and Welfare, NRL, Ministry of Education, Science and Technology

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