Preparation of Hot Water Extracts of Dandelion Leaves to Increase Anti-inflammatory Activity

항염증 활성이 증진된 민들레 잎의 열수 추출물 제조조건 설정

To materialize anti-inflammatory compounds from dandelion leaves, we studied optimal conditions of hot water extraction and concentration. We investigated the properties of extracts on various temperatures between 60 and $90^{\circ}C$. Extraction yields at $60^{\circ}C$ were the highest at $39.2{\pm}1.3%$ and flavonoid contents were increased by heating. However, anti-inflammatory activities were remarkably decreased by increasing temperature. According to the results that the highest anti-inflammatory activities of water extract were demonstrated at $60^{\circ}C$, cell wall degrading enzyme treatment was carried out to increase extraction yield of anti-inflammatory compounds. The enzyme treatment resulted in increased yields and flavonoids from dandelion leaves of 1.5-fold and anti-inflammatory activities were not decreased. Also, column chromatography was used for concentration of anti-inflammatory compounds from extract of dandelion leaves treated with enzyme. Anti-inflammatory activities of adsorbed compounds on Amberlite XAD-2 and Sepabeads SP-850 were significantly increased. However, yields of adsorbed compounds were increased over two-folds on Sepabeads SP-850 compared to Amberlite XAD-2. Thus Sepabeads SP-850 was more effective than Amberlite XAD-2 to concentrate the anti-inflammatory compounds of dandelion leaves.

민들레 잎으로부터 항염증 활성 물질을 분리하여 기능성 식품 소재로 개발하기 위해 민들레 잎의 적정 열수추출 및 항염증 활성물질의 농축조건을 조사하였다. $60{\sim}90^{\circ}C$의 추출온도에서의 추출물의 특성을 조사한 결과, $60^{\circ}C$ 추출물의 수율이 가장 높았고, flavonoid의 함량은 온도가 증가함에 따라 증가하였지만 항염증 활성은 감소하는 경향을 나타내었다. 가장 높은 항염증 활성을 나타낸 $60^{\circ}C$ 추출물의 유용성분의 추출을 증가시키기 위해 세포벽 분해 효소를 처리한 결과 민들레 잎에서 추출된 flavonoid 함량과 추출수율이 1.5배 증가하고 항염증 활성도 감소하지 않는 것으로 나타나 세포벽 분해효소 처리가 항염증 활성물질의 추출증가에 상당한 영향을 미치는 것으로 나타났다. 또한 효소 처리에 의한 민들레 잎 추출물로부터 추출한 항염증 활성물질을 농축시키기 위해 column chromatography를 실시한 결과, Amberlite XAD-2와 Sepabeads SP-850에 흡착된 물질 모두 항염증 활성이 매우 높은 것으로 나타났지만 Sepabeads SP-850에 흡착된 물질의 수율이 2배 이상 높았기 때문에 민들레 잎의 항염증 활성물질을 농축하는데 Sepabeads SP-850 resin이 훨씬 효과적임을 알 수 있었다.