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Biological Activities of Ethanol Extract from Corni fructus

산수유 에탄올 추출물의 생리활성

  • Kwon, Seong-Hyuk (Dept. of Food and Nutrition, Sunchon National University) ;
  • Yang, Hee-Sun (Dept. of Food and Nutrition, Sunchon National University) ;
  • Kim, Jae-Yong (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Park, Kyoung-Wuk (Dept. of Food and Nutrition, Sunchon National University) ;
  • Shon, Mi-Yae (Dept. of Food and Nutrition, Gyeongsang National University) ;
  • Kang, Kap-Suk (Dept. of Hotel Culinary Arts, Busan College of Information Technology) ;
  • Shim, Ki-Hwan (Division of Applied Sciences, Graduae School, Institute of Agricultured & Life Sciences, Gyeongsang National University) ;
  • Seo, Kwon-Il (Dept. of Food and Nutrition, Sunchon National University)
  • 권승혁 (순천대학교 식품영양학과) ;
  • 양희선 (순천대학교 식품영양학과) ;
  • 김재용 (경북대학교 식품공학과) ;
  • 박경욱 (순천대학교 식품영양학과) ;
  • 손미예 (경상대학교 식품영양학과) ;
  • 강갑석 (부산정보대학 호텔조리학과) ;
  • 심기환 (경상대학교 대학원 응용생명과학부.농업생명과학연구원) ;
  • 서권일 (순천대학교 식품영양학과)
  • Published : 2009.03.31

Abstract

In order to use Corni fructus as functional food materials, we investigated the biological activities of ethanol extracts from Corni fructus (EECF). The hydrogen-donating activity of EECF was increased in a dose dependent manner compared with untreated control, and the activities by EECF were 64 and 74% at 300 and $500{\mu}g/mL$ concentration, respectively. The NO productions in the RAW264.7 marcrophage cells treated with EECF were increased in dose dependent manners. EECF significantly inhibited the growth of MCF-7 human breast cancer cells in dose and time dependent manners. EECF of $500{\mu}g/mL$ concentration inhibited the proliferation by over 60% in the MCF-7 cells when treated for 72 hr. Also, the proliferations were increased in the MCF-7 cells cultured in the charcoal-treated FBS (cFBS) medium with environmental hormones such as bisphenol or $17{\beta}$-estradiol of $0.1{\mu}M$ whereas the proliferations were decreased in the MCF-7 cells treated with the environmental hormones after treatment of EEFC for 72 hr. The results suggest that Corni fructus would be used as functional food materials.

산수유를 기능성식품 소재로서 이용하기 위하여 산수유 에탄올추출물의 생리활성을 조사하였다. 산수유 에탄올추출물의 수소공여능은 농도 의존적으로 증가되었으며, 300 및 $500{\mu}g/mL$ 농도에서 각각 64, 73%의 활성을 나타내었다. 대식세포 RAW264.7에 산수유 에탄올추출물을 100, 300 및 $500{\mu}g/mL$의 농도로 처리하였을 때 산수유추출물은 농도 의존적으로 NO(nitric oxide)의 생성을 유도하였다. 또한 산수유 에탄올추출물을 유방암세포에 다양한 농도로 처리하여 24, 48, 72시간 반응시킨 결과 농도 및 시간에 의존적으로 유방암세포의 증식을 억제하였으며, $500{\mu}g/mL$ 농도로 72 시간 처리 시 60% 이상의 높은 증식억제율을 보여주었다. 또한, MCF-7 유방암 세포에 bisphenol과 $17{\beta}$-estradiol과 같은 환경호르몬을 $0.1{\mu}M$의 농도로 처리하였을 때 세포의 증식이 유도되었으며, 이 세포에 산수유 에탄올추출물을 농도별로 72시간 처리하였을 때 유방암 세포의 증식이 억제되었다. 따라서 본 연구결과는 산수유의 기능성식품 소재로서의 활용 가능성을 시사한다.

Keywords

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