Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Carotenoids from Green Alga Haematococcus pluvialis by HPLC and LC-MS (APCI) and Their Antioxidant Properties

  • Ranga, Rao (Plant Cell Biotechnology Department, Central Food Technological Research Institute (Constituent Laboratory of Council of Scientific and Industrial Research)) ;
  • Sarada, A.R. (Plant Cell Biotechnology Department, Central Food Technological Research Institute (Constituent Laboratory of Council of Scientific and Industrial Research)) ;
  • Baskaran, V. (Biochemistry and Nutrition Department, Central Food Technological Research Institute (Constituent Laboratory of Council of Scientific and Industrial Research)) ;
  • Ravishankar, G.A. (Plant Cell Biotechnology Department, Central Food Technological Research Institute (Constituent Laboratory of Council of Scientific and Industrial Research))
  • Published : 2009.11.30
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Abstract

Haematococcus pluvial is, a green alga, accumulates astaxanthin (3,3'-dihydroxy-$\beta$,$\beta$'-carotene-4,4'-dione) upto 2-3% on a dry weight basis. In the present study, identification of carotenoids from Haematococcus cyst cell extract by HPLC and LC-MS (APCI) and their antioxidant properties were evaluated in in vitro model systems. The extract exhibited 89% and 78% antioxidant activities in the $\beta$-carotene linoleate model and the hydroxyl radical scavenging model, at 9 ppm of total carotenoid, respectively. The extract also showed 80%, 85%, and 79% antioxidant activities against lipid peroxidation in the kidney, brain, and liver of rats. Low-density lipoprotein oxidation induced by $Cu^{2+}$ ions was also protected (45%, 64%, and 75%) by the extract in a dose-dependent manner with different carotenoid levels. Thiobarbituric acid reactive substances concentration in the blood, liver, and kidney of rats were also significantly (p<0.005) decreased in H. pluvialis-treated rats. The potent antioxidant activity is attributable to various carotenoids present in the extract.

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References

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