대한소아치과학회지 (Journal of the korean academy of Pediatric Dentistry)

  • 제36권4호
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  • Pages.531-538
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  • 2009
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  • 1226-8496(pISSN)
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  • 2288-3819(eISSN)
대한소아치과학회 (Korean Academy of Pediatric Dentistry)
권호 표지

자일리톨 섭취에 따른 Streptococcus mutans의 글루칸 생성관련 유전자 발현 억제효과

INHIBITION OF GLUCAN SYNTHESIS RELATED GENE EXPRESSION OF STREPTOCOCCUS MUTANS BY XYLITOL TREATMENT

  • 김지혜 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 이영은 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 안상헌 (동국대학교 의과대학 치과학교실) ;
  • 최연희 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 남순현 (경북대학교 치의학전문대학원 소아치과학교실) ;
  • 송근배 (경북대학교 치의학전문대학원 예방치과학교실)
  • Kim, Ji-Hye (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Lee, Young-Eun (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Ahn, Sang-Hun (Department of Dentistry, College of Medicine, Dongguk University) ;
  • Choi, Youn-Hee (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Nam, Soon-Heyun (Department of Pediatric Dentistry, School of Dentistry, Kyungpook National University) ;
  • Song, Keun-Bae (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
  • 발행 : 2009.11.30
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초록

본 연구에서는 장기적인 자일리톨의 섭취가 Streptococcus mutans의 대표적인 독성인자 중 하나인 글루칸 생성에 미치는 영향을 알아보기 위하여 글루칸 합성효소인 glucosyltansferase의 mRNA 발현을 실시간 역전사 중합효소 연쇄반응을 통해 평가하여 다음과 같은 결과를 얻었다. 1. 24개월 동안 자일리톨껌을 섭취한 군에서 타액 내 Streptococcus mutans의 colony 수는 통계적으로 유의하게 감소하였다(p<0.05). 2. 비수용성 글루칸 합성에 관여하는 유전자인 gtfB, gtfC의 발현은 자일리톨껌을 섭취한 군에서 시간이 지남에 따라 유의 하게 감소하였다(p<0.05). 특히 gtfB의 발현은 12개월과 24개월째 대조군에 비하여 통계적으로 유의하게 낮았고, gtfC의 발현은 24개월째 대조군에 비하여 통계적으로 유의하게 낮았다(p<0.05). 3. 수용성 글루칸 합성에 관여하는 유전자인 gtfD의 발현 역시 자일리톨껌을 섭취한 군에서 시간이 지남에 따라 유의하게 감소하였다(p<0.05). 또한 gtfD의 발현은 12개월과 24개월째 대조군에 비하여 통계적으로 유의하게 낮았다(p<0.05). 이상의 결과들을 종합해 보았을 때, 자일리톨의 섭취는 구강 내 Streptococcus mutans의 글루칸 합성 관련 유전자들의 발현을 억제시킴으로써 Streptococcus mutans의 수적인 감소를 가져오는 것으로 생각된다.

Xylitol has the ability to reduce the adherence of Streptococcus mutans(S. mutans), which can make it easier to remove plaque, decrease acid production and inhibit dental caries. There are few reports on the effects of xylitol on the expression of the virulence related genes in S. mutans. This study examined the inhibitory effect of chewing gum containing xylitol on glucan synthesis related gene expression of S. mutans. Participants were voluntarily recruited for a women's oral health prevention program, classified into two groups(a control and a xylitol group), and then followed for 2 years. Twenty salivary samples were randomly selected from each group. Colony count and real-time reverse transcription polymerase chain reaction were used to analyze the characteristics of S. mutans. The following results were obtained: The S. mutans counts decreased steadily in the xylitol group over the study period(p<0.05). The expression of the virulence related genes (gtfB, gtfC and gtfD) was significantly lower in the xylitol group than in the control groups (p<0.05). In conclusion, these results suggest that chewing xylitol gum for a long period of time may reduce the expression of the genes associated with S. mutans virulence, which can result in a decrease growth of S. mutans colonies as a result.

키워드

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