Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Stress Tolerance of Bifidobacterium infantis ATCC 27920 to Mild-heat Adaptation

  • Kang, Seok-U (Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Kim, Young-Hoon (Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Cho, In-Shick (Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Kang, Ja-Heon (Department of Ophthalmology, East-West Neo Medical Center, Kyung Hee University, College of Medicine) ;
  • Chun, Il-Byung (Animal Nutrition and Physiology, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Kwang-Hyun (Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Oh, Se-Jong (Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University)
  • Published : 2009.02.28
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Abstract

Two-dimensional gel electrophoresis (2-DE) was employed to assess the thermo-tolerance characteristics of Bifrdobacterium infantis ATCC 27920 to mild heat adaptation. When exposed to various heat levels, pH, and hydrogen peroxide ($H_2O_2$) stress conditions, B. infantis ATCC 27920 exhibited high level of stress resistance. Under mild-heat treatment ($46^{\circ}C$), no significant change in viability level was observed after 2 hr. Interestingly, improved viability was observed in mild-heat adapted ($46^{\circ}C$ for 1 hr) cultures exposed to $55^{\circ}C$, in comparison to control experiments. Viability was not affected by pH, bile, and $H_2O_2$ stress conditions. 2-DE analysis revealed those mild-heat adaptation up-regulated 4 proteins and down-regulated 3 proteins. Among these protein spots, isopropyhnalate dehydratase (leuD), glycosyltransferase (glgA), and ribosomal protein L5 (rp1E) were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALD1-TOF/MS).

Keywords

References

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