Morphological Characteristics of Intestine in Rats Fed Acidified Small Black Soybean

  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Ko, Byung-Moon (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Son, Geun-Seoup (Division of Biotechnology, College of Environmental and Bioresource Science, Chonbuk National University) ;
  • Jun, Hyun-Il (Faculty of Biotechnology, Institute of Agricultural Science and Technology, Chonbuk National University) ;
  • Kim, Young-Soo (Faculty of Biotechnology, Institute of Agricultural Science and Technology, Chonbuk National University)
  • 발행 : 2009.02.28

초록

In this study, the effects of processed small black soybeans on the intestinal morphological characteristics of rats were examined. Adult male rats were fed diets containing raw, cooked, or acidified small black soybean powders for 4 weeks. The total short chain fatty acid (SCFA) level was significantly higher in the acidified small black soybean supplemented group than in the raw and cooked soybean diet groups. The major SCFAs found in the experimental groups were acetate, followed by propionate and butyrate. The duodenal villus height and colonic mucosal thickness were also significantly higher in the acidified small black soybean supplemented group than in the raw and cooked soybean diet groups. The acidified small black soybean supplemented group showed the densest colonic mucosa by staining with alcian blue (AB), as compared to the raw and cooked soybean diet groups. The acidified small black soybean supplemented group exhibited strongly stained $CD4^+$ in the mucosal lamina propria, while cooked and acidified diet groups were more strongly stained $CD8^+$ in the submucosal lamina propria than the raw diet group. These results suggest that acidified small black soybeans may help improve intestinal function.

키워드

참고문헌

  1. Messina M, Persky VP, Barnes S. Soy intake and cancer risk- a review of the in vitro and in vivo data. Nutr. Cancer 21: 260-268 (1994)
  2. Knight DC, Eden JA. A review of the clinical effects of phytoestrogens. Obstet. Gynecol. 87: 897-904 (1996)
  3. Ali AA, Velasquez MT, Hansen CT, Mohamed AI, Bhathena SJ. Effects of soybean isoflavones, probiotics, and their interactions on lipid metabolism and endocrine system in an animal model of obesity and diabetes. J. Nutr. Biochem. 15: 583-590 (2004) https://doi.org/10.1016/j.jnutbio.2004.04.005
  4. Anderson JW, Smith BM, Washnock CS. Cardiovascular and renal benefits of dry bean and soybean intake. Am. J. Clin. Nutr. 70:464S-474S (1990)
  5. Tham DM, Gardner CD, Haskell WL. Potential health benefits of dietary phytoestrogens: A review of the clinical, epidemiological, and mechanistic evidence. J. Clin. Endocrinol. Metab. 83: 2223-2235 (1998) https://doi.org/10.1210/jc.83.7.2223
  6. Kim JS, Kim JG, Kim WJ. Changes of isoflavone contents in soybean cultivars pickled in persimmon vinegar. Korean J. Food Sci. Technol. 36: 833-836 (2004)
  7. Kim JA, Hong SB, Jung WS, Yu CY, Ma KH, Gwag JG, Chung IM. Composition of isoflavones composition in seed, embryo, cotyledon, and seed coat of cooked-with-rice and vegetable soybean vatieties. Food Chem. 102: 738-744 (2006) https://doi.org/10.1016/j.foodchem.2006.06.061
  8. Choung MG, Baek IY, Kang ST, Han WY, Shin DC, Moon HP, Kang KH. Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max L. Merr.). J. Agr. Food Chem. 49:5848-5851 (2001) https://doi.org/10.1021/jf010550w
  9. Lee CH, Oh SH, Yang EJ, Kim YS. Effects of raw, cooked, and germinated small black soybean powders on dietary fiber content and gastrointestinal functions. Food Sci. Biotechnol. 15: 635-638 (2006)
  10. Shinomiya K, Tokunaga S, Shigemoto Y, Kamei C. Effect of seed coat extract from black soybeans on radial maze performance in rats. Clin. Exp. Pharmacol. P. 32: 757-760 (2005) https://doi.org/10.1111/j.1440-1681.2005.04263.x
  11. Takahashi R, Ohmori R, Kiyose C, Momiyama Y, Ohsuzu F, Kondo K. Antioxidant activities of black and yellow soybeans against low density lipoprotein oxidation. J. Agr. Food Chem. 53: 4578-4582 (2005) https://doi.org/10.1021/jf048062m
  12. Tangerman A, Nagengast FM. A gas chromatographic analysis of fecal short-chain fatty acids, using the direct injection method. Anal. Biochem. 236: 1-8 (1996) https://doi.org/10.1006/abio.1996.0123
  13. Choi YK, Lee CH, Lee MW, Kwon J, Song GS, Kim YS. Effect of alcohol insoluble residues from stem and root barks of Ulmus davidiana on intestinal characteristics in rats. Food Sci. Biotechnol. 15: 380-384 (2006)
  14. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29: 577-580 (1981) https://doi.org/10.1177/29.4.6166661
  15. Lee CH, Yang EI, Song GS, Chai OH, Kim YS. Cheonggukjang mucilage stimulates immunohistochemical activities of gastrointestinal tract in rats. Food Sci. Biotechnol. 14: 813-817 (2005)
  16. Cummings JH. Short chain fatty acids in the human colon. Gut 22:763-779 (1981) https://doi.org/10.1136/gut.22.9.763
  17. Engelhardt WV, Ronnau K, Rechkemmer G, Sakata T. Absorption of short-chain fatty acids and their role in the hindgut of monogastric animals. Anim. Feed Sci. Tech. 23: 43-53 (1989) https://doi.org/10.1016/0377-8401(89)90088-6
  18. Davidson MH, McDonald A. Fiber: Forms and functions. Nutr. Res. 18: 617-624 (1998) https://doi.org/10.1016/S0271-5317(98)00048-7
  19. Schneeman BO. Fiber, inulin, and oligofructose: Similarits and differences. J. Nutr. 129: 1424S-1427S (1999) https://doi.org/10.1093/jn/129.7.1424S
  20. Sakata T. Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine: A possible explanation for for trophic effects of fermentable fiber, gut microbes, and luminal trophic factors. Brit. J. Nutr. 58: 95-103 (1987) https://doi.org/10.1079/BJN19870073
  21. Shimotoyome A, Meguro S, Hase T, Tokimitsu I, Sakata T. Short chain fatty acids but lactate or succinate stimulate mucus release in the rat colon. Compo Biochem. Phys. A 125: 525-531 (2000) https://doi.org/10.1016/S1095-6433(00)00183-5
  22. Kim YS, Tsao D, Siddiqui B, Whitehead JS, Arnstein P, Bennet J, Hicks J. Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon cancer cell. Cancer 45:1185-1192 (1980) https://doi.org/10.1002/1097-0142(19800315)45:5+<1185::AID-CNCR2820451324>3.0.CO;2-W
  23. Samanya M, Yamauchi K. Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Compo Biochem. Phys. A 133: 95-104 (2002) https://doi.org/10.1016/S1095-6433(02)00121-6
  24. Cross CE, Halliwell B, Allen A. Antioxidant protection- a function of tracheo-bronchial and gastrointestinal mucus. Lancet 323: 1328-1330 (1984) https://doi.org/10.1016/S0140-6736(84)91822-1
  25. Strugaka V, Allen A, Dettmar PW, Pearson JP. Colonic mucin:Methods of measuring mucus thickness. P. Nutr. Soc. 62: 237-243(2003) https://doi.org/10.1079/PNS2002205
  26. Satchithanandam S, Vargofcak-Apker M, Calvert RJ, Leeds AR, Cassidy MM. Alteration of gastrointestinal mucine by fiber feeding in rats. J. Nutr. 120: 1179-1184 (1990) https://doi.org/10.1093/jn/120.10.1179
  27. Shimotoyodome A, Meguro S, Hase T, Tokimitsu I, Sakata T. Sulfated polysaccharides, but not cellulose, increase colonic mucus in rats with loperomide-induced constipation. Digest. Dis. Sci. 46:1482-1489 (2001) https://doi.org/10.1023/A:1010644021888
  28. Matsuo K, Ota H, Akamatsu T, Sugiyama A, Katsuyama T. Histochemistry of the surface mucosal gel layer of the human colon. Gut 40: 782-789 (1997) https://doi.org/10.1136/gut.40.6.782
  29. Camerini V, Panwala C, Kronenberg M. Regional specialization of the mucosal immune system. J. Immunol. 151: 1765-1776 (1993)
  30. Nagai T, Ishizuka S, Hara H, Aoyama Y. Dietary sugar beet fiber prevents the increase in aberrant crypt foci induced by $\gamma$-irradiation in the colorectum of rats treated with an immunosuppressant. J. Nutr. 130: 1682-1687 (2000)
  31. Ishizuka S, Tanaka S. Modulation of $CD8^+$ intraepithelial lymphocyte distribution by dietary fiber in the rat large intestine. Exp. BioI. Med. 227: 1017-1021 (2002) https://doi.org/10.1177/153537020222701110