Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Genotoxic Evaluation of Surfactin C in Chinese Hamster Lung Cell Line

  • Published : 2009.03.01
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Abstract

To investigate the mutation inducibility of surfactin C, we performed the chromosome aberration assay with Chinese hamster lung cells in vitro. The colorimetric MTT screening assay was carried out to determine the cytotoxicity index ($IC_{50}$) of surfactin C. The $IC_{50}$ value was $125{\mu}g/ml$. For the chromosome aberration test of surfactin C, the maximum concentration was employed as $125{\mu}g/ml$, followed by 62.5 and $31.25{\mu}g/ml$ for the lower concentrations, with or without metabolic activation (S9). Cyclophosphamide and mitomycin C were used as positive controls in the presence and absence of S9 metabolic activation, respectively. These results showed that surfactin C was not capable of inducing chromosome aberration, as measured by the chromosome aberration test using Chinese hamster lung cell line. There is no evidence for surfactin C to have a genotoxic potential.

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References

  1. Arima, K., Kakinuma, A. and Tamura, G. (1968), Surfactin, a crystalline peptidolipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of fibrin clot formation, Biochem. Res. Commun., 31, 488-494 https://doi.org/10.1016/0006-291X(68)90503-2
  2. Banat, I.M., Makkar, R.S. and Cameotra, S.S. (2000), Potential commercial applications of microbial surfactants, Appl. Microbiol. Biotechnol., 53, 495-508 https://doi.org/10.1007/s002530051648
  3. Dearfield, K.L., Cimino, M.C., McCarroll, N.E., Mauer, I. and Valcovic, L.R. (2002), Genotoxicity risk assessment: a proposed classification strategy, Mutat. Res., 521, 121-135 https://doi.org/10.1016/S1383-5718(02)00236-X
  4. Desai, J.D. and Banat, I.M. (1997), Microbial production of surfactants and their commercial potential, Microbiol. Mol. Biol. Rev., 61, 47-64
  5. Galloway, S., Aardema, M., Ishidate, M., Ivett, J., Kirkland D., Morita, T., Mosesso, P. and Sofuni, T. (1994), Report from the working group on in vitro tests for chromosomal aberrations, Mutat. Res., 312, 241-262 https://doi.org/10.1016/0165-1161(94)00012-3
  6. Georgiou, G., Liu, S.C. and Sharma, M.M. (1990), Surface active compounds from microorganisms, Biotechnol., 10, 60-65 https://doi.org/10.1038/nbt0192-60
  7. Hwang, M.H., Lim, J.H., Kim, K.S., Rhee, M.H., Kim, N.W., Kim, J.C. and Park, S.C. (2005a), Antibacterial activity in vitro and primary dermal irritationtest in rabbits of surfactin produced in bacillus subtilus complex BC2121, J. Toxicol. Pub. Health, 20, 39-43
  8. Hwang, M.H., Lim, J.H., Yun, H.I., Rhee, M.H., Cho, J.Y., Hsu, W.H. and Park, S.C. (2005b), Surfactin C inhibits the lipopolysaccharide- induced transcription of interleukin-1beta and inducible nitric oxide synthase and nitric oxide production in murine RAW 264.7 cells, Biotechnol Lett., 27, 1605-1608 https://doi.org/10.1007/s10529-005-2515-1
  9. Hwang, Y.H., Park, B.K., Lim, J.H., Kim, M.S., Song, I.B., Park, S.C. and Yun, H.I. (2008), Evaluation of genetic and developmental toxicity of surfactin C from Bacillus subtilis BC1212, J. Health Sci., 54, 101-106 https://doi.org/10.1248/jhs.54.101
  10. Kanatomo, S., Nagai, S., Ohki, K. and Yasuda, Y. (1995), Study on surfactin, a cyclic depsipeptide. I. Isolation and structure of eight surfuctin analogs produced by Bacillus natto KMD 2311, Yakugaku. Zasshi., 115, 756-764 https://doi.org/10.1248/yakushi1947.115.9_756
  11. Kikuchi, T. and Hasumi, K. (2002), Enhancement of plasminogen activation surfactin C: augmentation of fibrinolysis in vitro and in vivo, Biochem. Biophys. Acta., 1596, 234-245 https://doi.org/10.1016/S0167-4838(02)00221-2
  12. Lim, J.H., Park, B.K., Kim, M.S., Hwang, M.H., Rhee, M.H., Park, S.C. and Yun, H.I. (2005), The anti-thrombotic activity of surfactins, J. Vet. Sci., 6, 353-355
  13. Mulligan, C.N. (2005), Environmental applications for biosurfactants, Environ Pollut., 133, 183-198 https://doi.org/10.1016/j.envpol.2004.06.009
  14. O'Brien, P.J., Hales, B.F., Josephy, P.D., Castonguay, A., Yamazoe, Y. and Guengerich, F.P. (1996), Chemical carcinogenesis, mutagenesis and teratogenesis, Can. J. Physiol. Pharmacol., 74, 565-571 https://doi.org/10.1139/cjpp-74-5-565
  15. Organization for Economic Cooperation and Development(OECD, 1997). Test Guideline 473, In vitro Mammalian chromosome aberration test. In: OECD Guidelines for the Testing of Chemicals. OECD Paris
  16. Park, B.K., Lim, J.H., Hwang, Y.H., Kim, M.S., Song, I.B., Lee, H.G., Han, S.J., Hwang, M.H., Kim, J.W., Park, S.C., Rhee, M.H. and Yun, H.I. (2006), Acute oral toxicity of surfactin C in mice, J. Toxicol. Pub. Health, 22, 453-458
  17. Rodrigues, L., Banat, I.M., Teixeira, J. and Oliveira, R. (2006), Biosurfactants: potential applications in medicine, J. Antimicrob.Chemother., 57, 609-618 https://doi.org/10.1093/jac/dkl024
  18. Singh, P. and Cameotra, S.S. (2004), Potential applications of microbial surfactants in biomedical sciences, Trends Biotechnol.,22, 142-146 https://doi.org/10.1016/j.tibtech.2004.01.010
  19. Scott, D., Danford, N., Dean, B., Kirkland, D. and Richardson C. (1983), In vitro chromosome aberration assays, Report of the UKEMS Sub-Committee on Guidelines for Mutagenicity Testing, Part 1, Cambridge University Press, Cambridge, UK, pp. 41-64
  20. Takahashi, T., Ohno, O., Ikeda, Y., Sawa, R., Homma, Y., Igarashi, M. and Umezawa, K. (2006), Inhibition of lipopolysaccharide activity by a bacterial cyclic lipopeptide surfactin, J. Antibiot (Tokyo), 59, 35-43 https://doi.org/10.1038/ja.2006.6