Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Entrapment of Milk Nutrients during Cholesterol Removal from Milk by Crosslinked β-Cyclodextrin

  • Ha, H.J. (Department of Food Science and Technology, Sejong University) ;
  • Jeon, S.S. (Department of Food Science and Technology, Sejong University) ;
  • Chang, Y.H. (Department of Food Science and Technology, Sejong University) ;
  • Kwak, H.S. (Department of Food Science and Technology, Sejong University)
  • Published : 2009.10.31
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Abstract

This study was designed to elucidate the quantities of milk nutrients entrapped during cholesterol removal from milk by crosslinked $\beta$-cyclodextrin ($\beta$-CD, 0.4~1.2%, w/v) and to evaluate the amounts of the residual $\beta$-CD in cholesterol-reduced milk treated by crosslinked $\beta$-CD. The content of lactose in the control milk (without treatment by crosslinked $\beta$-CD) was 4.86%, and the amounts of lactose entrapped by crosslinked $\beta$-CD ranged from 0.00 to 0.03%. The total amounts of the entrapped short-chain free fatty acid (FFA) and free amino acid (FAA) ranged from 0.03 to 0.09 ppm and from 0.28 to $0.71{\mu}mol/mL$, respectively. The amounts of the entrapped water-soluble vitamins (L-ascorbic acid, niacin, thiamine and riboflavin) ranged from 0.02 to 0.05 ppm, 0.01 to 0.06 ppm, 0.00 to 0.06 ppm and 0.01 to 0.06 ppm, respectively. The entrapped amounts of lactose, short-chain FFAs, FAAs and water-soluble vitamins were not remarkably affected by the concentrations of crosslinked $\beta$-CD (0.4~1.2%, w/v). Only very small amounts of residual $\beta$-CD in the cholesterol-removed milk were measured (1.22~3.00 ppm). Based on the data obtained from the present study, it was concluded that the amounts of entrapped nutrients were negligible during cholesterol removal from milk by crosslinked $\beta$-CD, and only trace amounts of residual $\beta$-CD were present in cholesterol-removed milk.

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References

  1. Adams, M. L., Sullivan, D. M., Smith, R. L., and Richer, E. F. (1986) Evaluation of direct saponification method in determination of cholesterol in meats. J. Assoc. Anal. Chem. 69, 844-846
  2. Alonso, L., Cuesta, P., and Gilliland, S. E. (2009) Effect of $\beta$-cyclodextrin on trans fats, CLA, PUFA and phospholipids of milk fat. J. Am. Oil. Chem. Soc. 86, 337-342 https://doi.org/10.1007/s11746-009-1355-x
  3. Cardwell, C. G. (1952) Industrial starch research. Chem. Eng. News 30, 514-517 https://doi.org/10.1021/cen-v030n006.p514
  4. Han, E. M., Kim, S. H., Ahn, J., and Kwak, H. S. (2005) Cholesterol removal from homogenized milk with crosslinked $\beta$-cyclodextrin by adipic acid. Asian-Austral. J. Anim. Sci. 18, 1794-1799 https://doi.org/10.5713/ajas.2005.1794
  5. Han, E. M., Kim, S. H., Ahn, J., and Kwak, H. S. (2007) Optimizing cholesterol removal from cream using $\beta$-cyclodextrin cross-linked with adipic acid. Int. J. Dairy Technol. 60, 31-36 https://doi.org/10.1111/j.1471-0307.2007.00296.x
  6. Hodgin, J. C., Howard, P. Y., and Ball, D. M. (1983) An automated device for in situ pre-column derivatization and injection of amino acids for HPLC analysis. J. Chromatogr. Sci. 221, 503-507
  7. Ikins, W. G., Kwak, H. S., Zink, G. S., and Jeon, I. J. (1988) Comparison of methods for quantitation of free fatty acids in cheese. J. Food Sci. 53, 1915-1916 https://doi.org/10.1111/j.1365-2621.1988.tb07878.x
  8. Kim, H. Y., Bae, H. Y., and Kwak, H. S. (2008) Development of cholesterol-reduced Blue cheese made by crosslinked $\beta$-cyclodextrin. Michwissenschaft. 63, 53-56
  9. Kim, J. J., Jung, T. H., Ahn, J., and Kwak, H. S. (2006) Properties of cholesterol-reduced butter made with β-cyclodextrin and added evening primrose oil and phytosterols. J. Dairy Sci. 89, 4503-4510 https://doi.org/10.3168/jds.S0022-0302(06)72498-5
  10. Kim, S. H., Han, E. M., Ahn, J., and Kwak, H. S. (2005) Effect of crosslinked β-cyclodextrin on quality of cholesterol-reduced cream cheese. Asian-Austral. J. Anim. Sci. 18, 584-589 https://doi.org/10.5713/ajas.2005.584
  11. Kwak, H. S. and Jeon, I. J. (1988) Comparison of high performance liquid chromatography and enzymatic method for the measurement of lactose in milk. J. Food Sci. 53, 975-976 https://doi.org/10.1111/j.1365-2621.1988.tb09002.x
  12. Lee, S. J., Hwang, J. H., Lee, S., Ahn, J., and Kwak, H. S. (2007) Property changes and cholesterol-lowering effects in evening primrose oil-enriched and cholesterol-reduced yogurt. Int. J. Dairy Technol. 60, 22-30 https://doi.org/10.1111/j.1471-0307.2007.00294.x
  13. Lindroth, P. and Mopper, K. (1979) High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescences derivatization with o-phthaldehyde. Anal. Chem. 52, 1667-1674
  14. Lopez, L. A., Alonso, P. C., and Gilliland, S. E. (2009) Analytical method for quantification $\beta$-cyclodextrin in milk, cream and butter by LC. Chromatographia 69, 1089-1092 https://doi.org/10.1365/s10337-009-1022-2
  15. Nagamoto, S. (1985) Cyclodextrin-expanding the development of their functions and applications. Chem. Econ. Engng. Rev. 17, 28-34

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