Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Takrisodokyeum Water Extracts on LNCaP Prostate Cancer Cells

  • Park, Kwan-Woo (Department of Obstetrics and Gynecology, School of Oriental Medicine, Wonkwnag University) ;
  • Kim, Song-Baeg (Department of Obstetrics and Gynecology, School of Oriental Medicine, Wonkwnag University) ;
  • Choi, Chang-Min (Department of Obstetrics and Gynecology, School of Oriental Medicine, Wonkwnag University) ;
  • Ryu, Do-Gon (Department of Physiology, School of Oriental Medicine, Wonkwnag University) ;
  • Kwon, Kang-Beom (Department of Physiology, School of Oriental Medicine, Wonkwnag University)
  • Published : 2009.10.25
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Abstract

Androgen receptors (AR) play a crucial role in the development and progression of prostate cancer. Many studies have suggested that prostate cancer cell proliferation is inhibited by AR downregulation, and it has been reported that Takrisodokyeum (TRSDY) induced apoptotic cell death and suppressed tumorigenesis in human leukemia cells. Therefore, this study was conducted to elucidate the mechanism by which TRSDY affects cell growth and AR expression in androgen-dependent prostate cancer cells (LNCaP cells). We investigated the proliferation and apoptosis of LNCaP cells using MTT and DNA fragmentation assays. In addition, we used western blot analysis to assess the effects of TRSDY on the expression of the AR target gene, prostate-specific antigen (PSA). Furthermore, the mechanism of AR downregulation by TRSDY was investigated using EMSA to analyze the binding activity of AR to androgen response elements (ARE). TRSDY significantly suppressed proliferation and induced apoptosis in LNCaP cells. In addition, TRSDY-induced apoptotic cell death was accompanied by activation of caspase-3 and cleavage of its substrate, poly(ADP-ribose) polymerase. TRSDY also inhibited the constitutively expressed- or 5a-dihydrotestosterone (DHT)-induced AR/PSA protein levels. However, these effects were mediated by inhibition of the binding of AR to ARE. TRSDY-mediated AR/PSA downregulation contributes to the inhibition of cell proliferation and the induction of apoptosis in LNCaP human prostate cancer cells. Our findings suggest that TRSDY may be used as a chemopreventive or chemotherapeutic agent for the treatment of prostate cancer.

Keywords

References

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