동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제23권5호
  • /
  • Pages.1139-1144
  • /
  • 2009
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

권삼(拳蔘)의 항암효과에 대한 연구

Studies of the Anti-cancer Effects of Bistortae Rhizoma

  • 김준범 (경원대학교 한의과대학 본초학교실) ;
  • 한효상 (경원대학교 한의과대학 본초학교실) ;
  • 이영종 (경원대학교 한의과대학 본초학교실)
  • Kim, June-Beom (Department of Herbology, College of Oriental Medicine, Kyungwon University) ;
  • Han, Hyo-Sang (Department of Herbology, College of Oriental Medicine, Kyungwon University) ;
  • Lee, Young-Jong (Department of Herbology, College of Oriental Medicine, Kyungwon University)
  • 발행 : 2009.10.25
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초록

This study was purposed to research the anti-cancer effects of Bistortae Rhizoma. A total extract of Bistortae Rhizoma decoction was prepared. By measuring the cell proliferation, apoptosis, morphology and cytokine level from the extracts, the influence on HepG2 cell, SNU-1 cell and A549 cell was compared. The Bistortae Rhizoma decoction extract did not control HepG2 cell proliferation but controlled SNU-1 cell and A549 cell proliferation. In particular, the inhibitory effect on SNU-1 cell proliferation was highest. The Bistortae Rhizoma decoction extract showed to increase the apoptosis of the HepG2 ceil, SNU-1 cell and A549 cell in a dose-dependent manner. In particular, the promotion effect of the apoptosis was highest in SNU-1 cell. Among the various fraction extracts of the Bistortae Rhizoma decoction, n-BuOH extraction showed the greatest increase of the apoptosis of the HepG2 cell. The Bistortae Rhizoma decoction extract decreased dose-dependently the secretion of the TGF-$\beta$ in the HepG2 cell, SNU-1 cell and A549 cell and increased the secretion of the TNF-$\alpha$ and the IFN-$\gamma$. These results suggest that the total extract of Bistortae Rhizoma decoction has anti-cancer effect against SNU-1 cell and A549 cell.

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참고문헌

  1. 孫星衍, 孫馮翼 輯. 神農本草經. 北京, 科學技術出版社, p 66, 1999
  2. 胡乃長, 王致譜 輯注. 圖經本草. 福建, 科學技術出版社, p 570, 1980
  3. 國家中醫藥管理局 中華本草 編委會. 中華本草. 上海, 科學技術出版社, 2: 643-645, 1999
  4. 식품의약품안전청. 대한약전외한약(생약)규격집. p 68, 2006
  5. 國家藥典委員會編. 中華人民共和國藥典 2005年版1部. 北京, 化學工業出版社, p 202, 2005
  6. 조선민주주의 인민공화국 보건부 약전위원회. 조선민주주의 인민공화국약전 제5판. 평양:의학과학출판사, p 189, 1996
  7. 江蘇新醫學院編. 中藥大辭典 下冊. 上海, 科學技術出版社, pp 1959-1960, 1977
  8. 中華人民共和國衛生部藥政管理局 中國藥品生物制品檢定所 編. 現代實用本草 上冊. 北京, 人民衛生出版社, p 652, 1996
  9. 編寫組. 全國中草藥會編 上冊. 北京, 人民衛生出版社, p 345, 653, 1990
  10. 徐樹楠 主編. 中藥臨床應用大全. 石家庄, 河北科學技術出版社, pp 102-103, 1998
  11. Duwiejua, M., Zeitlin, I.J., Gray, A.I., Waterman, P.G. Anti-inflammatory activity of Polygonum bistorta, Guaiacum officinale and Hamamelis virginiana in rats. Journal of Pharmacy and Pharmacology. 46(4):286-290, 1994 https://doi.org/10.1111/j.2042-7158.1994.tb03795.x
  12. Duwiejua, M., Zeitlin, I.J., Gray, A.I., Waterman, P.G. The anti-inflammatory compounds of Polygonum bistorta : Isolation and characterisation. Planta Medica. 65(4):371-374, 1999 https://doi.org/10.1055/s-2006-960791
  13. Smolarz, H.D., Skwarek, T. The investigations into the interferon-like activity of polygonum L. genus. Acta Poloniae Pharmaceutica Drug Research. 56(6):459-462, 1999
  14. 안중수. 拳參(Polygonum bistorta L.)의 소염활성 성분에 관한 연구. 강원대학교 석사학위논문, 1999
  15. 이동준. 권삼(拳參)이 지혈(止血), 소염(消炎)작용 및 중추신경계(中樞神經系)에 미치는 영향. 우석대학교 석사학위논문, 1996
  16. 尙志鈞 輯校. 名醫別錄. 北京, 人民衛生出版社, pp 248-249, 1986
  17. X.Q. Liu, H.M. Hua, J. Liu, F.K. Chen, L.J. Wu. A new tannin-related compound from the rhizome of Polygonum bistorta L. Journal of Asian Natural Products Research. 8(4):299-302, 2006 https://doi.org/10.1080/10286020500034956
  18. Manoharan, K.P., Benny, T.K.H., Yang, D. Cycloartane type triterpenoids from the rhizomes of Polygonum bistorta. Phytochemistry. 66(19):2304-2308, 2005 https://doi.org/10.1016/j.phytochem.2005.07.008
  19. Smolarz, H.D. Comparative study on the free flavonoid aglycones in herbs of different species of Polygonum. Acta Poloniae Pharmaceutica Drug Research. 59(2):145-148, 2002
  20. Smolarz, H.D. Flavonoid glycosides in nine Polygonum L. taxons. Acta Societatis Botanicorum Poloniae. 71(1):29-33, 2002 https://doi.org/10.5586/asbp.2002.004
  21. Pellegrion, M., Ferrone, S. and Theofilopoulos, A. Isolation of human T and B cells by rosette formation with 2-aminoethylisothiouronium bromide(AET)-treated sheep red blood cells and with monkey red blood cells. J. Immunol. Meth. 11: 273-277, 1976 https://doi.org/10.1016/0022-1759(76)90120-4
  22. Kishimoto, T., A.E.G., von dem Brorne, et al. Leucocyte typing VI: White cell differentiation antigens. Garland publishing, Inc. London. 1998
  23. Maiolini, R. and Masseyeff, R. Asandwigh method of enzymoimmunoassay. I. Appication to rat and human alpha-foetoprotein. J. Immunol. Meth. 8: 223-234, 1975 https://doi.org/10.1016/0022-1759(75)90115-5
  24. Evans, V.C. Multple pathways to apoptosis. Cell Biol Int. 17(5):461-476, 1993 https://doi.org/10.1006/cbir.1993.1087
  25. Shi, L., Nishioka, W.K., Th'ng, J., Bradbury, E.M. Litchfield DW Greenberg AH. Premature p34cdc2 activation required for apoptosis. Science. 263(5150):1143-1145, 1994 https://doi.org/10.1126/science.8108732
  26. 윤정구. 종양에 대한 생체방어기전, 대한의학협회지, 32(10): 1073-1077, 1989
  27. Rodriguez, G.C., Berchuck, A., Whitaker, R.S. Epidermal growth factor receptor expression in normal ovarian epithelium and ovarian cancer. II. Relationshop between receptor expression and response to epidermal growth factor. Am J Obstet Gynecol 164: 745-750, 1991 https://doi.org/10.1016/0002-9378(91)90508-O
  28. Roit, I.M., Brostoff, J., Male, D.K. Livingstone, C. Immunology. London, Gower Medical Publishing. 1989. 9.6-9.13
  29. Kakimi, K., Guidotti, L., Koezuka, Y., Chisari, F.V. Natural killer T cell activation inhibits hepatitis B virus replication in vivo. J Exp Med. 192: 921-930, 2000 https://doi.org/10.1084/jem.192.7.921