Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Fermented Red Ginseng Extracts on Physiological Activity and Blood Glucose Level in Streptozotocin Induced Diabetic Rats

홍삼발효 추출물의 생리활성 및 streptozotocin으로 유발된 당뇨쥐의 혈당강하에 미치는 영향

  • Kim, Hae-Ja (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Seo, Myeong-Hyo (College of Oriental Medicine, Wonkwang University) ;
  • Lee, Eun-Kyoung (College of Oriental Medicine, Wonkwang University) ;
  • Cho, Hwa-Eun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi, Yun-Hee (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ki-Nam (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chong, Myong-Soo (College of Oriental Medicine, Wonkwang University)
  • 김혜자 (원광대학교 한의학전문대학원) ;
  • 서명효 (원광대학교 한의과대학) ;
  • 이은경 (원광대학교 한의과대학) ;
  • 조화은 (원광대학교 한의학전문대학원) ;
  • 최윤희 (원광대학교 한의학전문대학원) ;
  • 이기남 (원광대학교 한의학전문대학원) ;
  • 정명수 (원광대학교 한의과대학)
  • Published : 2009.10.25
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Abstract

The purpose of this study was investigated hypoglycemic effects of fermented red ginseng extracts. We prepared non-fermented red ginseng extracts(R), fermented with Lactobacillus plantarum(RL) extracts, Saccharomycescerevisiae(RS) extracts, and L. plantarum mixed S. cerevisiae(RLS) extracts, examined composition of ginsenosides, SOD-like activity, and $\alpha$-glucosidase inhibitory activity. Ginsenoside Re was highest contents in all extracts, second was ginsenoside Rc and then ginsenoside Rb1. Concentration of these ginsenoside was showed higher in RS than in other extracts. SOD-like activity and $\alpha$-glucosidase inhibitory activity were shown higher in fermented red ginseng extracts than non fermented extracts. And activities of mixed fermentation extracts(RLS) higher than single fermentation extracts(RL, RS). Effects of blood glucose level, serum lipid profile and metabolic variables were evaluated in streptozotocin(STZ) induced diabetic rat. Experimental group was divided into 7 groups: normal control group(hereafter NC group), diabetes control group(DC group), positive control group treated with 50 mg/kg body weight of acarbose(PC group), treated with 300 mg/kg body weight of R, RL, RS and RLS extracts groups, respectively. Blood glucose level of DC group was maintained high level in all experimental period, but treated with red ginseng extracts groups was reduced the glucose level by R group 18.00%, RL group 28.07%, RS group 29.03%, RLS group 42.42%, respectively. The concentration of total cholesterol and triglyceride of fermented red ginseng extracts treated groups (RL, RS, RLS) was lower than non- fermented extracts group(R) DC and PC groups. The activity of ALT, AST in RLS treated groups were lower than other groups.

Keywords

References

  1. 이계준. 발효미생물학, 서울, 라이프사이언스, p 3, 2002
  2. Shah, N.P. Functional foods from probiotics and prebiotics, Food Technol, 55: 46-53, 2005
  3. Kim, N.Y., Han, M.J. Development of Ginseng yoghurt fermented by Bifidobacterium spp, Korean J Food Cookery Sci, 21: 575-584, 2005
  4. Lee, I.S., Paek, K.Y. Preparation and quality characteristics of yoghurt added with cultured ginseng, Korean J Food Sci Techol, 35: 235-241, 2003
  5. Goh, J.S., Chae, Y.S., Gang, C.G., Kwon, I.K., Choi, M., Park, H. Effect of ginseng extract on the acid production by lactic acid bacteria and the distribution of intestinal microflora of mouse, J Dairy Sci, 15: 216-225, 1996
  6. Koo, H.H., Chung, S.H. Effects of Panax ginseng and Ganoderma lucidum extract on the growth of lactic acid bacteria, J Korean Soc Food Sic Nutr, 7: 45-50, 1994
  7. Hong, O.S., Ko, Y.T. Study on preparation of yogurt from milk and rice, Korean J Food Sci Technol, 23: 587-592, 1991
  8. Yoon, K.Y., Wooddams, E.E., Hang, Y.D. Production of probiotic cabbage juice by lactic acid bacteria, Bioresource Technol, 97: 1427-1430, 2006 https://doi.org/10.1016/j.biortech.2005.06.018
  9. Lee, E.H., Nam, E.S., Park, S.L. Characteristics of curd yogurt from milk added with Maesil (Pruns mume), Korean J Food Sci Technol, 34: 419-424, 2002
  10. Lee, I.S., Lee, S.O., Kim, H.S. Preparation and quality characteristics of yoghurt added with Saururus chinensis (Lour.)bail. J Korean Soc Food Nutr, 31: 411-416, 2002 https://doi.org/10.3746/jkfn.2002.31.3.411
  11. Han, M.J., Lee, Y.K. Development of yogurt containing pumpkin. Kor J Food Sci Techol, 35: 173-175, 1993
  12. Jung, Y.J., Han, D.H., Choi, B.H., Park, C., Lee, H., Kim, S.H., Hahm, D.H. Effect of fermented herbal extracts, HP-1 on enzyme activities and gene expressions related to alcohol metabolism in ethanol-loaded rats, Korean Journal of Oriental Physiology and Pathology, 21: 387-391, 2007
  13. 전국한의학대학 공동교재편찬위원회. 본초학, 서울, 영림사, p 576, 2004
  14. Nam, K.Y. Clinical Applications and Efficacy of Korean Ginseng (Panax ginseng C.A. Meyer), J Ginseng Res, 26(3):111-131, 2002 https://doi.org/10.5142/JGR.2002.26.3.111
  15. Park, J.D. Recent studies on the chemical constituents of Korea ginseng, Korean J Ginseng Sci, 20: 389-415, 1996
  16. 고인호, 장재철, 고정삼. 방사선이 조사된 생쥐 간에서 백삼과 발효인삼추출물이 단백질 및 지질과산화에 미치는 효과, 대한방사선기술학회지 27: 1226-2854, 2004
  17. 김동현. 인삼과 건강, 서울, 도서출판 효일, pp 31-33, 2005
  18. Han, J.Y., Goo, D.H., Han, M.S., Ryu, G.H. Comparison of fermentability and characteristics of fermented broth for white ginseng, red ginseng and extruded white ginseng. Food Engineering Progress, 11: 119-126, 2007
  19. Park, S.J., Kim, D.H., Paek, N.S., Kim, S.S. Preparation and quality characteristics of the fermentation product of ginseng by lactic acid bacteria (FGL), Korean J Ginseng Sci, 30(2):88-94, 2006 https://doi.org/10.5142/JGR.2006.30.2.088
  20. Lau, A.J., Woo, S.O., Koh, H.L. Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection, J Chromatogr A, 1011: 77-87, 2003 https://doi.org/10.1016/S0021-9673(03)01135-X
  21. Sung, H.S., Yang, C.B. Effect of ethanol concentration on saponin composition of red ginseng extract, Korean Journal of Food Science and Technology, 17: 227-231, 1985
  22. Yokozawa Kobayashi, T., Oura, H., Kawashima, Y. Studies on the mechanism of the hypoglycemic activity of ginsenoside-Rb2 in streptozotocin-diabetic rats, Chem Pharm Bull, 33: 869-872, 1985 https://doi.org/10.1248/cpb.33.869
  23. Attele, A.S., Zhou, Y.P., Xie, J.T., Wu, J.A., Zhang, L., Dey, L., Pugh, W., Rue, P.A., Polonsky, K.S., Yuan, C.S. Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component, Diabetes, 51(6):1851-1858, 2002 https://doi.org/10.2337/diabetes.51.6.1851
  24. Mochizuki, M., Yoo, Y.C., Matsuzawa, K., Sato, K., Saiki, I., Tono-Oka, S., Samukawa, K.I., Azuma, I. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside-Rb2, 20(R)-and 20(S)-ginsenoside-Rg3, of red ginseng, Biol Pharm Bull, 18: 1197-1202, 1995 https://doi.org/10.1248/bpb.18.1197
  25. Han, B.H., Park, M.H., Han, Y.N., Shin, C.S. Studies on the antioxidant components of Korean ginseng (IV) antifatigue active components, Yakhak Hoeji, 28: 231-235, 1984
  26. Choi, C.S., Kim, K.I., Hong, H.D., Choi, S.Y., Lee, Y.C. Phenolic acid composition and antioxidative of white ginseng, J Ginseng Res, 30: 22-30, 2006 https://doi.org/10.5142/JGR.2006.30.1.022
  27. Gua, J., Jin, Y.S., Han, W., Shim, T.H., Sa, J.H., Wang, M.H. Studies for component analysis, antioxidative activity and $\alpha$-glucosidase inhibitory activity from equisetum arvense, J Kor Soc Appl Biol Chem, 49(1):77-81, 2006
  28. The Diabetes Conrol and Complication Trial (DCCT) Research Group : Effects of intensive treatment of long-term complication in insulin-dependent diabetes mellitus, N Eng J Med, 329: 977-986, 1993 https://doi.org/10.1056/NEJM199309303291401
  29. Shichiri, M. Long term results of th Gumamoto study on optimal diabetes control in type 2 diabetic patients. Diabetes Care Suppl, 2: B21-29, 2000
  30. UK Prospective Diabetes Study (UKPDS) Group : Intensive blood-glucose control with control with sulphonylueas or insulin compared with conventional treatment and risk of complications in patients with type 2 diabetes (UKPDS 33), Lancet, 352: 837-853, 1998 https://doi.org/10.1016/S0140-6736(98)07019-6
  31. Waki, I., Kyo, H., Yasuda, M., Kimura, M. Effects of a hypoglycemic component of ginseng radix on insulin biosynthesis in normal and diabetic animals, J Pharmcobiodyn, 5: 547-554, 1982 https://doi.org/10.1248/bpb1978.5.547
  32. Sievenpiper, J.L., Sung, M.K., Di Buono, M., Lee, K.S., Nam, K.Y., Arnason, J.T., Leiter, L.A., Vuksan, V. Korean red ginseng rootlets decrease acute postprandial glycemia: Results from sequential preparation-and dose-finding studies, J Am Coll Nutr, 25: 100-107, 2006 https://doi.org/10.1080/07315724.2006.10719519
  33. Sotaniemi, E.A., Haapakoski, E., Rautio, A. Ginseng therapy in non-insulin-dependent diabetic patients, Diabetes Care, 18: 1373-1375, 1995 https://doi.org/10.2337/diacare.18.10.1373
  34. Lee, W.K., Kao, S.T., Liu, I.M., Cheng, J.T. Increase of insulin secretion by ginsenoside Rh2 to lower plasma glucose in Wistar rats, Clin Exp Pharmacol Physiol, 33: 27-32, 2006 https://doi.org/10.1111/j.1440-1681.2006.04319.x
  35. Cho, W.C., Chung, W.S., Lee, S.K., Leung, A.W., Cheng, C.H., Yue, K.K. Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats, Eur J Pharmacol, 550(1-3):173-179, 2006 https://doi.org/10.1016/j.ejphar.2006.08.056
  36. Trinh, H.T., Han, S.J., Kim, S.W., Lee, Y.C., Kim, D.H. Bifidus fermentation increases hypolipidemic and hypoglycemic effects of red ginseng, J Microbiol Biotechnol, 17(7):1127-1133, 2007