Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Single Oral Dose Toxicity Test of Taeumjowi-tang in ICR Mice

태음조위탕(太陰調胃湯)의 마우스 단회투여 독성시험

  • Kim, Jong-Dae (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University)
  • 김종대 (대구한의대학교 한의과대학 폐계내과학교실)
  • Published : 2009.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

The object of this study was to evaluate the single dose toxicity of Taeumjowui-tang(TUJWT), a polyherbal formula have been traditionally used as prevention or treatment agent for obesity, in male and female mice. Aqueous extracts of TUJWT (Yield = 10.5%) was administered to female and male ICR mice as an oral dose of 2000, 1000 and 500 mg/kg (body wt.) according to the recommendation of KFDA Guidelines. Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy, organ weight and histopathology of 12 principle organs were examined. As results, we could not find any mortality, clinical signs, and changes in the body and organ weight except for diarrhea restricted in TUJWT 2000 mg/kg treated one male mouse at 1 day after administration, and increases of popliteal lymph node weights in all TUJWT administered groups. In addition, no TUJWT-treatment related abnormal gross findings and changes in histopathology of principle organs were detected except for increases of the frequency of hypertrophy and hyperplasia of lymphoid cells in the popliteal lymph nodes in all TUJWT treatment groups with some sporadic accidental findings. These increases of popliteal lymph node weights with hypertrophy and hyperplasia of lymphoid cells were considered that related to the immune modulate effect of TUJWT not toxicological signs. The results obtained in this study suggest that the TUJWT does not cause any toxicological signs. The 50% lethal dose and approximate lethal dose of TUJWT aqueous extracts in both female and male mice were considered as over 2000 mg/kg.

Keywords

References

  1. 박용주. 한방정책의 현재와 미래. 한국한의학연구원 국제학술심포지움, 1998
  2. Lee, J.E., Kim, H.J., Choi, E.K., Chai, H.Y., Yun, Y.W., Kim, D.J., et al. Four-week repeated-dose toxicity study on Pinellia Extract, Korean J. Lab. Anim. Sci., 19: 127-141, 2003
  3. 이제마. 東醫壽世保元, 서울, 대성문화사, pp 19-24, 137-141, 1993
  4. 전국한의과대학 사상의학회. 四象醫學. 서울, 집문당, pp 413, 436-438, 553-555, 1998
  5. 전병훈, 김경요, 이기주. 太陰調胃湯이 白鼠의 肥滿症 및 誘導肥滿細胞에 미치는 效果. 동의병리학회지 10: 88-98, 1996
  6. 송미연, 이종수, 김성수, 신현대. 節食療法 施行 후 太陰人肥滿에 대한 淸肺瀉肝湯과 太陰調胃湯의 臨床的 硏究. 韓方再活醫學科學會誌 8: 34-56, 1998
  7. 김만우, 박성식. 太陰調胃湯의 抗酸化 效能에 의한 肝細胞 保護 效果에 관한 硏究. 사상체질의학회지 13: 51-60, 2001
  8. 신동준, 김달래, 김선형. 太陰調胃湯과 麻黃이 肥滿 白鼠의 Leptin에 미치는 影響. 사상체질의학회지 13: 79-87, 2001
  9. 윤진환, 류성환, 정경훈, 최덕구, 정일규, 이희혁 외. 운동과 太陰調胃湯 처방이 흰쥐의 간기능 효소와 간내 지방축적에 미치는 영향. 운동과학, pp 345-357, 2002
  10. 장문석, 심경준, 김휴영, 박은화, 고은빛, 최봉재 외. SD계 흰쥐에 대한 太陰調胃湯의 급성 경구 독성 시험 연구. 동의생리병리학회지 22(6):1439-1443, 2008
  11. 한국식품의약품안전청. 의약품 등의 독성시험기준. 한국식품의약품 안전청 고시 제 2005-60호, 2005
  12. Irwin, S. Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse. Psychopharmacology, 13: 222-257, 1968 https://doi.org/10.1007/BF00401402
  13. Dourish, C.T. Effects of drugs on spontaneous motor activity. In Experimental Psychopharmacology (A.J. Greenshaw and C.T. Dourish, Ed.), Clifton, Humana Press, pp 325-334, 1987
  14. Klein, H.O., Kreysch, H.G., Coerper, C., Voigt, P., Ruff, I. Preclinical and early clinical trial with mafosfamide as immune modulator. Methods Find Exp. Clin. Pharmacol. 9: 627-640, 1987
  15. Duarte, C.G., dos Santos, G.L., Azzolini, A.E., de Assis Pandochi, A.I., The effect of the antithyroid drug propylthiouracil on the alternative pathway of complement in rats. Int. J. Immunopharmacol., 22: 25-33, 2000 https://doi.org/10.1016/S0192-0561(99)00061-2
  16. Iqbal, M.P., Sultana, F., Mehboobali, N. and Pervez, S. Folinic acid protects against suppression of growth by methotrexate in mice. Biopharm. Drug Dispos, 22: 169-178, 2001 https://doi.org/10.1002/bdd.271
  17. Pinzone Fox, M.L., Sastry, M.K., Parenti, D.M., Simon, G.L. Plasma leptin concentration increases early during highly active antiretroviral therapy for acquired immunodeficiency syndrome, independent of body weight. J. Endocrinol. Invest., 28: 1-3, 2005 https://doi.org/10.1007/BF03345520
  18. Plata, E.J., Murphy, W.H. Growth and haematologic properties of the BALB/wm strain of inbred mice. Lab. Anim. Sci. 22: 712-720, 1972
  19. Yamaguchi, C., Fujita, S., Obara, T., Ueda, T. Effects of room temperature on reproduction, body weight and organ weights, food and water intakes, and hematology in mice. Exp. Anim., 32: 1-11, 1983 https://doi.org/10.1538/expanim1978.32.1_1
  20. Hsu, H.Y., Lin, B.F., Lin, J.Y., Kuo, C.C., Chiang W. Suppression of allergic reactions by dehulled adlay in association with the balance of TH1/TH2 cell responses. J Agric Food Chem. 51: 3763-3769, 2003 https://doi.org/10.1021/jf021154w
  21. Zhu, Y.P. Chinese Materia Medica Chemistry, Pharmacology and Applications. The Netherlands, Harwood Academic Publisher, pp 629-632, 1998
  22. 박진홍, 이현수, 문형철, 김대호, 성낙술, 정해곤 외, 초음파 병행 추출의 마황과 복분자 열수 추출물의 면역 활성 증진에 미치는 효과. 한국생물공학회지 19: 113-117, 2004
  23. 김대호, 박진홍, 김정화, 김철희, 유진현, 권민철 외. 저온 추출 공정에 의한 마황과 복분자의 면역 활성 증진 효과. 한국약용작물학회지 13: 81-86, 2005
  24. 김정화, 김대호, 유진현, 김철희, 권민철, 성낙술 외, 초음파 병행 추출을 이용한 마황과 복분자, 당귀 분획물의 면역활성 조절 효과. 한국약용작물학회지 13: 161-170, 2005
  25. Lee, J,H., Yang, K.J., Shin, H.D., Park, B.R., Son, C.W., Jang, H.J., et al, Single subcutaneous dose toxicity of Polycan, a -glucan originated from Aureobasidium in mice. Lab. Anim. Res., 21: 299-305, 2005
  26. Lee, H.S., Lee, I.G., Ku, S.K. Single oral dose toxicity study of water extracts of Picrorrhiza Rhizoma in mice. J. Toxicol. Pub. Health, 22: 117-126, 2006
  27. Banks, W.J. Female reproductive system. In: Banks WJ (Ed). Applied veterinary histology, 2nd ed. Baltimore, Williams & Wilkins, pp 506-526, 1986
  28. Pineda, M.H. Female reproductive system. In: McDonald LE and Pineda MH (Eds). Veterinary endocrinology and reproduction, Philadelphia, Lea & Febiger, pp 303-354, 1989
  29. U.S. Environmental Protection Agency. Health Effects Test Guidelines OPPTS 870.100, Acute Toxicity Testing Background. US EPA August, Washington, USA, 1998