Imaging Science in Dentistry

Korean Academy of Oral and Maxillofacial Radiology (대한영상치의학회)
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Effects of 2-deoxy-D-glucose and quercetin on the gene expression of bone sialoprotein and osteocalcin during the differentiation in irradiated MC3T3-E1 osteoblastic cells

2-deoxy-D-glucose와 quercetin이 방사선조사 MC3T3-E1 골모세포주의 분화시 bone sialoprotein과 osteocalcin 유전자의 발현에 미치는 영향

  • Lee, Ji-Un (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bioscience, Chonbuk National University) ;
  • Kim, Kyoung-A (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bioscience, Chonbuk National University) ;
  • Koh, Kwang-Joon (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bioscience, Chonbuk National University)
  • 이지운 (전북대학교 치의학전문대학원 구강악안면방사선학교실 및 구강생체과학연구소) ;
  • 김경아 (전북대학교 치의학전문대학원 구강악안면방사선학교실 및 구강생체과학연구소) ;
  • 고광준 (전북대학교 치의학전문대학원 구강악안면방사선학교실 및 구강생체과학연구소)
  • Published : 2009.09.30
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Abstract

Purpose : To investigate the effects of 2-deoxy-D-glucose (2-DG) and quercetin (QCT) on gene expression of bone sialoprotein (BSP) and osteocalcin (OC) during the differentiation in irradiated MC3T3-E1 osteoblastic cells. Materials and Methods : When MC3T3-E1 osteoblastic cells had reached 70-80% confluence, cultures were transferred to a differentiating medium supplemented with 5 mM 2-DG or $10{\mu}M$ QCT, and then irradiated with 2, 4, 6, and 8 Gy. At various times after irradiation, the cells were analyzed for the synthesis of type I collagen, and expression of BSP and OC. Results : The synthesis of type I collagen in cells exposed to 2 Gy of radiation in the presence of 2-DG or QCT showed no significant difference compared with the control group within 15 days post-irradiation. When the cells were irradiated with 8 Gy, 2-DG facilitated the irradiation mediated decrease of type I collagen synthesis, whereas such decrease was inhibited by treating with QCT. During MC3T3-E1 osteoblastic cell differentiation, the mRNA expression of BSP and OC showed the peak value at 14 days and 21 days, respectively. 2-DG or QCT treatment alone decreased the level of BSP mRNA, but increased the OC mRNA level only at early time of differentiation (day 7). In the cells irradiated with 2, 4, 8 Gy, the mRNA expression of BSP and OC decreased at 7 days after the irradiation. The cells were treated with various dose of radiation in the presence of 2-DG or QCT, the mRNA level of both BSP and OC increased although this increase was observed at low dose of radiation (2 Gy) and at the early stage of differentiation. However, when the cells were exposed to 4, 6, or 8 Gy, the increase of BSP and OC mRNAs was detected only in cells co-incubated with QCT. Conclusion : This study demonstrates that 2-DG and QCT affect differently the expression of bone formation related factors, type I collagen, BSP, and OC in the irradiated MC3T3-E1 osteoblasic cells, according to the dose of radiation and the times of differentiation. Overall, the present findings suggest that 2-DG and QCT could have the regulatory roles as radiation-sensitizer and -protector, respectively.

Keywords

References

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