Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Immunomodulatory Effect of Pueraria lobata on the Functional Activation of Macrophages by Lipopolysaccharide Treatment

그람음성균 유래 lipopolysaccharide로 활성화된 대식세포의 기능 수행시 갈화 [Pueraria lobata (Willd.) Ohwi]의 조절작용

  • Shen, Ting (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Jong-Dai (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Cho, Jae-Youl (School of Bioscience and Biotechnology, Kangwon National University)
  • 센팅 (강원대학교 BT학부대학 생명공학부) ;
  • 김종대 (강원대학교 BT학부대학 생명공학부) ;
  • 조재열 (강원대학교 BT학부대학 생명공학부)
  • Published : 2009.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Pueraria lobata (Willd.) Ohwi was investigated to check its modulatory effects on the activation of macrophages upon inflammatory conditions treatment. For this purpose, we examined several inflammatory responses such as nitric oxide (NO) production, reactive oxygen species (ROS) generation, cytoprotection and phagocytosis under the treatment of methanol extract from P. lobata (Pl-ME). Pl-ME dose-dependently blocked NO production in lipopolysaccharide (LPS)- stimulated RAW264.7 cells but not sodium prusside (SNP)-generated NO release. The NO inhibition seemed to be due to blocking inducible NO synthase (iNOS), since Pl-ME suppressed its expression in a NF-${\kappa}B$-independent manner. Similarly, this extract also effectively protected RAW264.7 cells from LPS-induced cytotoxicity. However, Pl-ME did not block ROS generation and rather it enhanced. Finally, this extract negatively modulated FITC-dextran uptake. Therefore, our data suggested that Pl-ME may be involved in negatively regulating some macrophage-mediated inflammatory responses such as NO production and phagocytic uptake.

Keywords

References

  1. Arao T, Kinjo J, Nohara T and Isobe R. (1997). Oleanene-type triterpene glycosides from puerariae radix. IV. Six new saponins from Pueraria lobata. Chemical and Pharmaceutical Bulletin (Tokyo). 45:362-366 https://doi.org/10.1248/cpb.45.362
  2. Chen F and Shi X. (2002). NF-kappaB, a pivotal transcription factor in silica-induced diseases. Molecular Cell Biochemistry. 234-235: 169-176 https://doi.org/10.1023/A:1015915000265
  3. Chen G, Zhang J and Jiannong Y. (2001). Determination of puerarin, daidzein and rutin in Pueraria lobata (Wild.) Ohwi by capillary electrophoresis with electrochemical detection. Journal of Chromatography A. 923:255-262 https://doi.org/10.1016/S0021-9673(01)00996-7
  4. Cho JY, Baik KU, Jung JH and Park MH. (2000). In vitro antiinflammatory effects of cynaropicrin, a sesquiterpene lactone, from Saussurea lappa. European Journal of Pharmacology. 398:399-407 https://doi.org/10.1016/S0014-2999(00)00337-X
  5. Cho JY, Grigura V, Murphy TL and Murphy K. (2003). Identification of cooperative monomeric Brachyury sites conferring T-bet responsiveness to the proximal IFN-gamma promoter. International Immunology. 15:1149-1160 https://doi.org/10.1093/intimm/dxg113
  6. Dijkstra G, Moshage H and Jansen PL. (2002). Blockade of NFkappaB activation and donation of nitric oxide: new treatment options in inflammatory bowel disease? Scandinavian Journal of Gastroenterology. Supplement:37-41
  7. Edwards JP, Zhang X, Frauwirth KA and Mosser DM. (2006). Biochemical and functional characterization of three activated macrophage populations. Journal of Leukocyte Biology. 80: 1298-1307 https://doi.org/10.1189/jlb.0406249
  8. Fang H, Pengal RA, Cao X, Ganesan LP, Wewers MD, Marsh CB and Tridandapani S. (2004). Lipopolysaccharide-induced macrophage inflammatory response is regulated by SHIP. Journal of Immunology. 173:360-366 https://doi.org/10.4049/jimmunol.173.1.360
  9. Hsu FL, Liu IM, Kuo DH, Chen WC, Su HC and Cheng JT. (2003). Antihyperglycemic effect of puerarin in streptozotocininduced diabetic rats. Journal of Natural Products. 66:788-792 https://doi.org/10.1021/np0203887
  10. Hwang YP, Choi CY, Chung YC, Jeon SS and Jeong HG. (2007). Protective effects of puerarin on carbon tetrachlorideinduced hepatotoxicity. Archieves of Pharmacal Research. 30:1309-1317 https://doi.org/10.1007/BF02980272
  11. Jung KK, Lee HS, Cho JY, Shin WC, Rhee MH, Kim TG, Kang JH, Kim SH, Hong S and Kang SY. (2006). Inhibitory effect of curcumin on nitric oxide production from lipopolysaccharide-activated primary microglia. Life Sciences. 79:2022-2031 https://doi.org/10.1016/j.lfs.2006.06.048
  12. Katiyar SK, Matsui MS, Elmets CA and Mukhtar H. (1999). Polyphenolic antioxidant (−.)-epigallocatechin-3-gallate from green tea reduces UVB-induced inflammatory responses and infiltration of leukocytes in human skin. Photochemistry and Photobiology. 69:148-153
  13. Lee YG, Byeon SE, Kim JY, Lee JY, Rhee MH, Hong S, Wu JC, Lee HS, Kim MJ, Cho DH and Cho JY. (2007). Immunomodulatory effect of Hibiscus cannabinus extract on macrophage functions. Journal of Ethnopharmacology. 113:62-71 https://doi.org/10.1016/j.jep.2007.04.019
  14. Lee YG and Cho JY. (2007). Inhibitory effect of curcumin on nitirc oxide production in lipopolysaccharide-stimulated RAW264.7 cells and its suppressive mechanism. Korean Journal of Medicinal Crop Science. 15:451-456
  15. Lee YG, Lee WM, Kim JY, Lee JY, Lee IK, Yun BS, Rhee MH and Cho JY. (2008). Src kinase-targeted antiinflammatory activity of davallialactone from Inonotus xeranticus in lipopolysaccharide-activated RAW264.7 cells. British Journal of Pharmacology. 154:852-863 https://doi.org/10.1038/bjp.2008.136
  16. Migliaccio E, Giorgio M and Pelicci PG. (2006). Apoptosis and aging: role of p66Shc redox protein. Antioxidants & Redox Signaling. 8:600-608 https://doi.org/10.1089/ars.2006.8.600
  17. Miyazawa M, Sakano K, Nakamura S and Kosaka H. (2001). Antimutagenic activity of isoflavone from Pueraria lobata. Journal of Agricultural and Food Chemistry. 49:336-341 https://doi.org/10.1021/jf000255w
  18. Savina A and Amigorena S. (2007). Phagocytosis and antigen presentation in dendritic cells. Immunological Reviews. 219:143-156 https://doi.org/10.1111/j.1600-065X.2007.00552.x
  19. Shen T, Lee YJ and Cho JY. (2008). Effect of Hot Water Extract from Scutellaria barbata on the Macrophages Activated by Lipopolysaccharide. Korean Journal of Medicinal Crop Science. 16:313 319
  20. Tetley TD. (2005). Inflammatory cells and chronic obstructive pulmonary disease. Current Drug Targets: Inflammation and Allergy. 4:607-618 https://doi.org/10.2174/156801005774912824
  21. Zhang G and Fang S. (1997). Antioxidation of Pueraria lobata isoflavones (PLIs). Zhong Yao Cai. 20:358-360