Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Characterization of Bio-active Materials from Prickly Lettuce (Lactuca serriola)

가시상추 유래 생리활성물질의 분리 및 특성 규명

  • Moon, Sung-Il (Institute of Health and Environment, Gyeongsangbukdo Government) ;
  • Kim, Sung-Whan (Institute of Health and Environment, Gyeongsangbukdo Government) ;
  • Huh, Wan (Institute of Health and Environment, Gyeongsangbukdo Government) ;
  • Kim, Soon-Young (Dept. of Biological Sciences, Andong National University) ;
  • Kim, Jong-Sik (Dept. of Biological Sciences, Andong National University) ;
  • Lee, Kon-Joo (Dept. of Biological Sciences, Andong National University)
  • 문성일 (경상북도 보건환경연구원) ;
  • 김성환 (경상북도 보건환경연구원) ;
  • 허완 (경상북도 보건환경연구원) ;
  • 김순영 (안동대학교 자연과학대학 생명과학과) ;
  • 김종식 (안동대학교 자연과학대학 생명과학과) ;
  • 이건주 (안동대학교 자연과학대학 생명과학과)
  • Published : 2009.02.28
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Abstract

The bio-active materials were isolated from prickly lettuce (Lactuca serriola) by using several extraction solvents. The contents of general compounds, vitamins, polyphenols and flavonoids of prickly lettuce were analysed. In addition, nitrite scavenging activity, anti-oxidative activity and anti-microbial activity of methanol extracts were measured. The ethylacetate (EtOAc) fraction of methanol extracts of prickly lettuce showed the best on the nitrite scavenging activity, anti-oxidant activity and anti-microbial activity. And also, the contents of polyphenols and flavonoids were the highest among all fractions. The subfraction 2 (EtOAc:MeOH=3:1) of EtOAc fraction showed the strongest anti-oxidative activity among 5 subfractions. The subfraction 2 was identified as ${\rho}$-coumaric acid and caffeic acid by GC-MS. The anti-microbial activity of each solvent fraction from prickly lettuce was measured. The EtOAc fraction showed the strongest anti-microbial activity. Overall, these results may provide the basic data to understand the biological activities of bio-active materials derived from prickly lettuce.

가시상추(Prickly lettuce : Lactuca serriola)로부터 여러 가지 추출용매를 사용하여 생리활성물질을 분리하였다. 가시상추의 일반성분, vitamin, polyphenol성 물질 그리고 flavonoid 성분의 함량을 분석하였다. 또한, MeOH 조추출물에 의한 아질산염 제거 활성, 항산화 활성, 항균 활성을 측정하였다. MeOH 조추출물의 EtOAc 분획에서 가장 높은 아질산염 제거 활성, 항산화 활성 그리고 항균 활성을 보여주었다. 그리고 polyphenol성 물질과 flavonoid의 함량도 가장 높았다. EtOAc 분획 중 subfraction 2 (EtOAc:MeOH=3:1)에서 가장 높은 항산화 활성을 보여주었다. Subfraction 2는 GC-MS에 의해 ${\rho}$-coumaric acid와 caffeic acid로 동정되었다. 가시상추 추출물의 용매 분획별 항균 활성을 검색한 결과, EtOAc 분획에서 가장 높은 항균 활성을 보여주었다. 이러한 연구 결과는 가시상추 유래의 생리활성물질의 생물학적 활성을 이해하는데 필요한 기본 데이터를 제공해 줄 것이다.

Keywords

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