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Haematococcus pluvialis로부터 Haematococcus 추출물 제조 공정에서 효소 처리가 추출 효율과 항산화 활성에 미치는 영향

Effect of Enzyme Treatments on the Extraction Efficacy and Antioxidant Activity of Haematococcus Extract from Haematococcus pluvialis

  • 발행 : 2009.01.31

초록

우수한 항산화제로 알려진 astaxanthin을 함유한 H. pluvialis 균체로부터 작용기작이 상이한 exe형과 endo형의 단백질 분해 효소와 복합 다당류 분해 효소를 이용하여 식품용 haematococcus추출물을 효율적으로 제조할 수 있는 방법을 조사하였다. 상업용 단백질 분해 효소로는 Alcalase (endo형)와 Flavourzyme (exe형)을 복합 다당류 분해효소로는 Viscozyme을 사용하였다. 단백질 분해 효소는 endo형과 exe형을 병용하는 것이 추출물의 astaxanthin 함량을 증가시켰다. Viscozyme과 함께 2종류의 단백질 분해 효소를 사용하는 경우에는 Alcalase와 Flavourzyme을 병용하여 1차로 처리한 후 Viscozyme을 2차로 사용하는 2단계 가수분해 방법이 적절하였다. 이 조건에서 astaxanthin 함량은 효소를 사용하지 않은 대조구에 비하여 320% ($529{\mu}g/g{\rightarrow}2,256{\mu}g/g$) 이상 향상되었다. 또한 DPPH법으로 조사한 항산화 활성은 astaxanthin 함량에 비례하여 증가하였으며, 1차로 Alcalase와 Flavourzyme을 병용하여 처리한 후 2차로 Viscozyme을 사용하는 조건에서 가장 우수하였다.

An efficient production method of food-grade heamatococcus extract was developed based on stepwise enzymatic hydrolysis. In the first step, Haematococcus pluvialis cells hydrolysis carried out with commercially available exopeptidase(Flavourzyme) and endopeptidase (Alcalase), resulted in increased astaxanthin content. In the second step, proteolytic hydrolyzed H. pluvialis cells treated with hetero-polysaccharides hydrolytic enzyme (Viscozyme). By two-stage treatments using Alcalase and Flavourzyme and Viscozyme, the highest astaxanthin content was obtained. The astaxanthin content was remarkably enhanced by 320% $(529{\mu}g/g\rightarrow2,256{\mu}g/g)$ than that of the non-treated extract. And then, antioxidative activities determined by DPPH method were increased with increasing the astaxanthin content in haematococcus extract prepared by enzymatic hydrolysis.

키워드

참고문헌

  1. Guerin, M., Huntley, M. E. and Olaizola, M. (2003) Haematococcus astaxanthin: applications for human health and nutrition. Trends Biotechnol. 21, 210-216. https://doi.org/10.1016/S0167-7799(03)00078-7
  2. Kobayashi, M., Kakizono, T., Nishio, N., Nagai, S., Kurimura, Y. and Tsuji, Y. (1997) Antioxidant role of astaxanthin in the green algae Haematococcus pluvialis. Appl. Microbiol. Biotechnol. 48, 351-356. https://doi.org/10.1007/s002530051061
  3. Jyonouchi, H., Sun, S., Iijima, K., and Gross, M. (2000) Antitumor activity of astaxanthin and its mode of action. Nutr. Cancer 36, 59-65. https://doi.org/10.1207/S15327914NC3601_9
  4. Higuera-Ciapara, I., Felix-Vanlenzuela, L. and Goycoolea, F. M. (2006) Astaxanthin: a review of its chemistry and applications. Crit. Rev. Food Sci. Nutr. 46, 185-196. https://doi.org/10.1080/10408690590957188
  5. Schroeder, W. A., and Johnson, E. A. (1993) Antioxidant role of carotenoids in Phaffia rhodozyma. J. Gen. Microbiol. 39, 907-912.
  6. Orosa, M., Franqueira, D., Cid, A. and Abalde, J. (2005) Analysis and enhancement of astaxanthin accumulation in Haematococcus pluvialis. Bioresource Technol. 96, 373-378. https://doi.org/10.1016/j.biortech.2004.04.006
  7. Kobayashi, M., Kurimura, Y., Kakizono, T., Nishio, N. and Tsuji, Y. (1997) Morphological changes in the life cycle of the green algae Haematococcus pluvialis. J. Ferment. Bioeng. 84, 94-97. https://doi.org/10.1016/S0922-338X(97)82794-8
  8. Kang, C. D. and Sim, S. J. (2007) Selective extraction of free astaxanthin from Haematococcus culture using a tandem organic solvent system. Biotechnol. Prog. 23, 866-871 https://doi.org/10.1002/bp0700354
  9. Valderrama, J. O., Perrut, M. and Majewski, W. (2003) Extraction of astaxanthin and phycocyanine from microalgae with supercritical carbon dioxide. J. Chem. Eng. Data 48, 827-830. https://doi.org/10.1021/je020128r
  10. Machmudah, S., Shotipruk, A., Goto, M., Sasaki, M. and Horose, T. (2006) Extraction of astaxanthin from Haematococcus pluvialis using supercritical $CO_2$ and ethanol entrainer. Ind. Eng. Chem. Res. 45, 3652-3657. https://doi.org/10.1021/ie051357k
  11. In, M. -J., Choi, J. -H., Kim, S., Chae, H. J. and Kim, D. H. (2008) Enhanced extraction of astaxanthin from Haematococcus pluvialis using enzyme treatment. J. Korean Soc. Appl. BioI, Chem. 51, 247-249.
  12. Blois, M. S. (1958) Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1203. https://doi.org/10.1038/1811199a0
  13. Orosa, M., Franqueira, D., Cid, A. and Abalde, J. (2005) Analysis and enhancement of astaxanthin accumulation in Haematococcus pluvialis. Bioresource Technol. 96, 373-378. https://doi.org/10.1016/j.biortech.2004.04.006
  14. Kim, S., Cho, E., In, M. -J. and Chae, H. J. (2008) Extraction and analysis of astaxanthin from Haematococcus pluvialis using sonication. J. Korean Soc. Food Sci. Nutr. 37, 1363-1368. https://doi.org/10.3746/jkfn.2008.37.10.1363
  15. In, M. -J., Jang, J. E. and Kim, D. H. (2007) Enhancing extraction yield of chlorella extract by enzyme treatment. J. Appl. BioI. Chem. 50, 132-135.
  16. Wang, S. -B., Hu, Q., Sommerfield, M. and Chen, F (2004) Cell wall proteomices of the green algae Haematococcus pluvialis(Chlorophyceae). Proteomics 4, 692-708. https://doi.org/10.1002/pmic.200300634
  17. Chae, H. J., Joo, H. and In, M. -J. (2001) Utilization of brewer’s yeast cells for the production of food-grade yeast extract. Part 1: effects of different enzymatic treatments on solid and protein recovery and flavor characteristics, Bioresource Technol., 76, 253-258. https://doi.org/10.1016/S0960-8524(00)00102-4
  18. In, M. -J., Chae, H. J. and Oh, N. -S. (2002) Process development for heme-enriched peptide by enzymatic hydrolysis of hemoglobin. Bioresource Technol. 84, 63-68. https://doi.org/10.1016/S0960-8524(02)00009-3
  19. Kobayashi, M., Kurimura, Y., Sakamoto, Y. and Tsuji, Y. (1997) Selective extraction of astaxanthin and chlorophyll from the green algae Haematococcus pluvialis. Biotechnol. Tech. 11, 657-660. https://doi.org/10.1023/A:1018455209445
  20. Park, E. -K., Seo, M.-W. and Lee, C. -G. (2001) Effects of medium compositions for the growth and the astaxanthin production of Haematococcus pluvialis. Kor. J. Appl. Microbiol. Biotechnol. 29, 227-233.

피인용 문헌

  1. Astaxanthin-Producing Green Microalga Haematococcus pluvialis: From Single Cell to High Value Commercial Products vol.7, pp.1664-462X, 2016, https://doi.org/10.3389/fpls.2016.00531