Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Growth retardation and differential regulation of expansin genes in chilling-stressed sweetpotato

  • Noh, Seol Ah (School of Life Sciences and Biotechnology, Korea University) ;
  • Park, Sun Hee (School of Life Sciences and Biotechnology, Korea University) ;
  • Huh, Gyung Hye (Department of Molecular and Biomedical Technology, Inje University) ;
  • Paek, Kyung-Hee (School of Life Sciences and Biotechnology, Korea University) ;
  • Shin, Jeong Sheop (School of Life Sciences and Biotechnology, Korea University) ;
  • Bae, Jung Myung (School of Life Sciences and Biotechnology, Korea University)
  • Received : 2008.09.23
  • Accepted : 2008.10.23
  • Published : 2009.02.28
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Abstract

We report here a first evaluation of chilling-responsive gene regulation in the sweetpotato. The growth of sweetpotato plants was severely retarded at $12^{\circ}C$; the lengths of the leaf, petiole, and root were markedly reduced and microscopic observation revealed that the elongation growth of the epidermal cells in each of these organs was significantly reduced. We examined the transcriptional regulation of three sweetpotato expansin genes (IbEXP1, IbEXP2 and IbEXPL1) in response to various chilling temperatures (12, 16, 22, and $28^{\circ}C$). In the leaf and petiole, the highest transcript levels were those of IbEXP1 at $28^{\circ}C$, whereas IbEXPL1 transcript levels were highest in the root. IbEXP1 mRNA levels in the $12^{\circ}C-treated$ petiole showed a fluctuating pattern (transient decrease-recovery-stable decrease) for 48 h. In the leaf and petiole, IbEXP1 and IbEXPL1 exhibited a similar response to chilling in that their mRNA levels decreased at $22^{\circ}C$, increased at $16^{\circ}C$, and decreased dramatically at $12^{\circ}C$. In contrast, mRNA levels of IbEXP2 in the leaf fell gradually as the temperature fell from 28 to $12^{\circ}C$, while they remained unaltered in the petiole. In the root, mRNA levels of IbEXPL1 and IbEXP1 reached maximum levels at $16^{\circ}C$, and decreased significantly at $12^{\circ}C$. These data demonstrated that expression of these three expansin genes was ultimately down-regulated at $12^{\circ}C$; however, transcriptional regulation of each expansin gene exhibited its own distinctive pattern in response to various chilling temperatures.

Keywords

Acknowledgement

Supported by : Rural Development Administration, Korea Science and Engineering Foundation

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