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Elicitor-induced accumulation of stilbenes in cell suspension cultures of Cayratia trifolia (L.) Domin

  • Roat, Chetana (Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University) ;
  • Ramawat, K.G. (Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University)
  • Received : 2008.06.16
  • Accepted : 2009.01.10
  • Published : 2009.04.30

Abstract

Cell cultures of Cayratia trifolia (Vitaceae), a tropical lianas, were maintained in Murashige and Skoog's medium containing $0.25mg\;1^{-1}$ NAA, $0.2mg\;1^{-1}$ kinetin and casein hydrolysate $250mg\;1^{-1}$. Cell suspension cultures of C. trifolia accumulate stilbenes (piceid, resveratrol, viniferin, ampelopsin), which on elicitation by any of $500{\mu}M$ salicylic acid, $100{\mu}M$ methyl jasmonate, $500{\mu}M$ ethrel and $500mg\;1^{-1}$ yeast extract, added on the 7th day, were enhanced by 3- to 6-fold ($5-11mg\;1^{-1}$) by the 15th day.

Keywords

Acknowledgement

Supported by : UGC-DRS

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