Annals of Hepato-Biliary-Pancreatic Surgery (한국간담췌외과학회지)

The Korean Association of Hepato-Biliary-Pancreatic Surgery (한국간담췌외과학회)
권호 표지

Microsatellite Instability and p53 Gene Loss of Heterozygosity in Hepatocellular Carcinoma

간세포암에서 현미부수체 불안정성과 p53 유전자 이형접합 소실

  • Kim, Jun-Seok (Department of Surgery, Hallym University College of Medicine) ;
  • Lim, Man-Sup (Department of Surgery, Hallym University College of Medicine) ;
  • Kim, Doo-Jin (Department of Surgery, Hallym University College of Medicine) ;
  • Kim, Joo-Seop (Department of Surgery, Hallym University College of Medicine) ;
  • Kim, Kwan-Seok (Department of Surgery, Hallym University College of Medicine) ;
  • Kim, Hong-Ki (Department of Surgery, Hallym University College of Medicine) ;
  • Cho, Seong-Jin (Department of Pathology, Hallym University College of Medicine) ;
  • Kwon, Mi-Jung (Department of Pathology, Hallym University College of Medicine) ;
  • Nam, Eun-Sook (Department of Pathology, Hallym University College of Medicine) ;
  • Choi, Kyung-Chan (Department of Pathology, Hallym University College of Medicine) ;
  • Shin, Hyung-Sik (Department of Pathology, Hallym University College of Medicine) ;
  • Chae, Gi-Bong (Department of Surgery, Kangwon National University College of Medicine)
  • 김준석 (한림대학교 의과대학 외과학교실) ;
  • 임만섭 (한림대학교 의과대학 외과학교실) ;
  • 김두진 (한림대학교 의과대학 외과학교실) ;
  • 김주섭 (한림대학교 의과대학 외과학교실) ;
  • 김관석 (한림대학교 의과대학 외과학교실) ;
  • 김홍기 (한림대학교 의과대학 외과학교실) ;
  • 조성진 (한림대학교 의과대학 병리학교실) ;
  • 권미정 (한림대학교 의과대학 병리학교실) ;
  • 남은숙 (한림대학교 의과대학 병리학교실) ;
  • 최경찬 (한림대학교 의과대학 병리학교실) ;
  • 신형식 (한림대학교 의과대학 병리학교실) ;
  • 채기봉 (강원대학교 의과대학 외과학교실)
  • Published : 2009.09.30

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Abstract

Purpose: Hepatocellular carcinoma (HCC) shows various molecular and genetic alterations in its development and progression. Recently, microsatellite instability (MSI) and the loss of heterozygosity (LOH), have been postulated as useful prognostic factors in many malignant tumors. LOH is related to the allelic loss of various tumor suppressor genes, however, MSI has been found to be the result of a mismatched ONA pairing. Our objectives were to evaluate MSI and p53 gene LOH and to correlate this to clinicopathological factors. Methods: MSI analysis was performed by using polymerase chain reaction with 5 microsatellite markers (BAT25, BAT26, D2S123, D5S346 and D17S250 recommended in the 1998 NCI International Workshop) on 50 surgically resected tumors. p53 LOH was detected with 4 markers (D17S796, TP53, D17S5, D17S513). Results: MSI and p53 LOH were detected in 30% and 66%, respectively. 18% of HCCs exhibited MSI in 5 NCI-recommended markers and 18% of HCCs demonstrated MSI in 4 p53 markers. MSI was mostly detected in BAT25 and BAT26 markers. MSI was more frequently detected in tumor grade 1, small HCC, and non-lymphovascular group. For the most part, p53 LOH was detected by D17S513 marker (38.1%). p53 LOH results were correlated with higher tumor grade and invasiveness. LOH-High group showed a significant correlation with advanced HCCs and lymphovascular invasion. There was no demonstrated correlation between MSI and p53 LOH was not demonstrated. Conclusion: These results suggest that MSI may be involved to some extent in hepatocarcinogenesis and tumor invasion. Also MSI and p53 gene LOH may be a useful clinical indicator in determining the prognosis among patients with HCC.

Keywords

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