Annals of Hepato-Biliary-Pancreatic Surgery (한국간담췌외과학회지)

The Korean Association of Hepato-Biliary-Pancreatic Surgery (한국간담췌외과학회)
권호 표지

The Factors Affecting Transplanted Hepatocytes Repopulation in Rats with Liver Fibrosis

간섬유화가 유도된 백서에서 이식된 간세포 착상과 증식에 관여하는 인자

  • Lee, Sang-Woo (Department of Surgery, Yonsei University College of Medicine) ;
  • Min, Seon-Ok (Department of Surgery, Yonsei University College of Medicine) ;
  • Choi, Sae-Byeol (Department of Surgery, Korea University College of Medicine) ;
  • Kim, Kyung-Sik (Department of Surgery, Yonsei University College of Medicine)
  • 이상우 (연세대학교 의과대학 외과학교실) ;
  • 민선옥 (연세대학교 의과대학 외과학교실) ;
  • 최새별 (고려대학교 의과대학 외과학교실) ;
  • 김경식 (연세대학교 의과대학 외과학교실)
  • Published : 2009.12.31

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Abstract

Purpose: Cell therapy for various diseases has gained wide acceptance. Because most patients with chronic liver failure have mild-to-severe liver cirrhosis, there are many limitations to clinical applications. We analyzed how to increase cell engraftment in rats with liver fibrosis. Methods: We used analbuminemic SD rats (NARs) as recipients of syngeneic CAG-EGFP SD hepatocytes obtained by the 2 perfusion method. Hepatic fibrosis was induced with thioacetamide in drinking water for 6 weeks in the recipient NARs. NARs were pre-treated with gadolinium, doxorubicin, and gliotoxin before hepatocyte transplantation. We evaluated the degree of cell engraftment by RT-PCR and immunofluorescent staining for GFP and albumin. The transplanted cells were detected by immunostaining for albumin, and serum albumin was also measured. Results: Although detection of GFP by RT-PCR was variable, albumin was detected in all groups 4 wks after hepatocyte transplantation. GFP and albumin were also detected by immunofluorescent staining 1 and 4 wks after cell transplantation. In control rats, albumin production was maximal at 3 wks, and after that it rapidly decreased. In the gadolinium and doxorubicin-treated group, albumin production was increased up to 4 wks. Albumin production in the gadolinium-treated group was superior to that of the doxorubicin-treated group. Conclusion: Kupffer cells play the most important role in cell engraftment in hepatic fibrosis. Therefore, perturbation of kupffer cells in hepatic fibrosis is needed to increase cell engraftment.

Keywords

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