Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Effects of Treadmill Exercise on Inhibition of Soleus Muscle Atrophy and Improvement Functional Recovery after Spinal Cord Contusion in the Rats

척수타박손상 후 트레드밀운동이 근 위축지연과 기능적 회복에 미치는 효과

  • Oh, Myung-Jin (Department of Sports Science, Hannam University) ;
  • Jang, Moon-Nyeo (Department of Sports Science, Hannam University) ;
  • Seo, Tae-Beom (Department of Sports Science, Hannam University) ;
  • Kim, Jong-Oh (Department of Sports Science, Hannam University) ;
  • Byun, Jae-Jong (Department of Health and Fitness Management, Woosong University) ;
  • Yoon, Jin-Hwan (Department of Sports Science, Hannam University) ;
  • Jeong, Il-Gyu (Department of Sports Science, Hannam University)
  • 오명진 (한남대학교 생활체육학과) ;
  • 장문녀 (한남대학교 생활체육학과) ;
  • 서태범 (한남대학교 생활체육학과) ;
  • 김종오 (한남대학교 생활체육학과) ;
  • 변재종 (우송대학교 스포츠건강관리학부) ;
  • 윤진환 (한남대학교 생활체육학과) ;
  • 정일규 (한남대학교 생활체육학과)
  • Published : 2009.11.30
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Abstract

Physical activity and exercise can promote sensorimotor recovery from central nerve injury. It has been suggested that the functional recovery promoted by exercise training after spinal cord injury might be associated with insulin-like growth factor-I in the inflicted muscle. To investigate morphological and biochemical change of the soleus muscle after spinal cord injury, all tissues were used for H&E, immunofluorescence staining and Western blot. Also, BBB-test was used to evaluate behavioral improvement after spinal cord contusion. Thirty male Sprague-Dawley rats ($230{\pm}10\;g$; 7week in age) were assigned equally to three different groups; Normal (n=10), SCI (n=10), SCI+TMT (n=10). Every rat in SCI and SCI+TMT groups underwent laminectomy at T9 level and then contusion on the exposed spinal cord site in anesthetized condition. After one week-recovery from contusion, every rat in the SCI+TMT group exercised on a motorized treadmill for 30min/d, 5d/wk for 7wks. TMT followed by injury increased IGF-I induction levels in the soleus muscle and inhibited muscle atrophy. Behavioral scales for 4 and 8 weeks after spinal cord injury were improved in the SCI+TMT group compared to the SCI group. These results suggest that treadmill exercise after spinal cord injury might promote functional recovery along with muscle regrowth through the up-regulation of IGF-1 in muscle tissue.

본 연구는 흰쥐의 흉추 9번을 타박 손상시키고 1주간의 휴식 후 스트레스를 유발하지 않는 저강도 트레드밀운동을 7주동안 수행하여, 가자미근 위축지연 변화를 western blotting과 H&E 염색 그리고 면역형광염색법으로 관찰하였으며, BBB-검사를 이용하여 기능적 회복을 관찰하였다. 형태학적 변화의 결과 척수손상 후 트레드밀 운동에 의해 하지부위 가자미 근과 근섬유의 횡단면 크기가 증가하였으며, 근섬유 세포에서 IGF-I 단백질 발현이 증가하였다. 또한 생화학적인 결과 척수손상 후 트레드밀 운동에 의해 하지부위 골격근 IGF-I 단백질 발현이 증가하였으며, 행동학적 검사를 통해 트레드밀 운동그룹이 비 운동그룹보다 기능적 회복이 개선된 것을 확인하였다. 이러한 결과는 근 비대에 직접적으로 관여하는 IGF-I 단백질 발현량이 운동에 의해 증가하여 가자미근크기 증가와 같은 근 위축 지연을 확인하였으며, 이러한 변화를 통해 기능적 회복에 긍정적인 결과를 보여주었다. 따라서 운동은 척수손상 환자에게 효과적인 기능재활 치료방법이 될 수 있으며, 장애를 최소화하여 자발적인 일상생활을 도모할 수 있을 것이라 생각된다.

Keywords

References

  1. Adams, G. Rand S. A. McCue. 1998. Localized infusion of IGF-1 results in skeletal muscle hypertrophy in rats. J. Appl. Physiol. 84, 1716-1722
  2. Allen, D. L., J. K. Linderman, R. R. Roy, A. J. Bigbee, R. E. Grindeland, V. Mukku, and V. R. Edgerton. 1997. Apoptosis: a mechanism contributing to remodeling of skeletal muscle in response to hindlimb unweighting. Am. J. Physiol. Cell Physiol. 273, C579-C587
  3. Allen, D. L., S. R. Monke, R. J. Talmadge, R. R. Roy, and V. R. Edgerton. 1995. Plasticity of myonuclear number in hypertrophied and atrophied mammalian skeletal muscle fibers. J. Appl. Physiol. 78, 1969-1976
  4. Allen, D. L. W., Yasui, T. Tanaka, Y. Ohira, S. Nagaoka, C. Sekiguchi, W. E. Hinds, R. R. Roy, and V. R. Edgerton. 1996. Myonuclear number and myosin heavy chain expression in rat soleus single muscle fibers after spaceflight. J. Appl. Physiol. 81, 145-151
  5. Barton-Davis, E. R., D. I. Shoturma, A. Musaro, N. Rosenthal, and H. L. Sweeney. 1998. Viral mediated expression of insulin-like growth factor I blocks the aging-related loss of skeletal muscle function. Prac. Natl. Acad. Sci. USA. 95, 15603-15607 https://doi.org/10.1073/pnas.95.26.15603
  6. Basso, D. M. M. S. Beattie, and J. C. Bresnahan. 1995. A sensitive and reliable locomotor rating scale for open field testing in rats. J. Neuratrauma 12, 1-21 https://doi.org/10.1089/neu.1995.12.1
  7. Basso, D. M., M. S. Beattie, and J. C. Bresnahan. 1996. Graded histological and locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus transection. Exp. Neural. 139, 244-56 https://doi.org/10.1006/exnr.1996.0098
  8. Bedford, T. G, C. M., Tipton, N. C., Wilson, R. A., Oppliger, and C. V. Gisolfi. 1979. Maximum oxygen consumption of rats and its changes with various experimental procedures. J. Appl. Physiol. 47, 1278-1283
  9. Cabric, M. and N. T. James. 1983. Morphometric analyses on the muscles of exercise trained and untrained dogs. Am. J. Anat. 166, 359-368 https://doi.org/10.1002/aja.1001660309
  10. Castro, M. J., D. F. Jr. Apple, E. A. Hillegass, and G. A. Dudley. 1999. Influence of complete spinal cord injury on skeletal muscle cross-sectional area within the first 6 months of injury. Eur. J. Appl. Physiol. Occup. Physiol. 80, 373-378 https://doi.org/10.1007/s004210050606
  11. Coleman, M. E. F. Yin., DeMayo, K. C. Lee, H. M. R. Geske, C. Montgomery, and R. J. Schwartz. 1995. Myogenic vector expression of insulin-like growth factor I stimulates muscle cell differentiation and myofiber hypertrophy in transgenic mice. J. BioI. Chem. 270, 12109-12116 https://doi.org/10.1074/jbc.270.20.12109
  12. Darr, K. C. and E. Schultz. 1989. Hindlimb suspension suppresses muscle growth and satellite cell proliferation. J. Appl. Physiol. 67, 1827-1834
  13. Dijkstra, S., S. Duis, I. M. Pans, A. J. Lankhorst, F. P. Hamers, H. Veldman, P. R. Bar, W. H. Gispen, E. A. Joosten, and E. E. Jr. Geisert. 2006. Intraspinal administration of an antibody against CD81 enhances functional recovery and tissue sparing after experimental spinal cord injury. Exp. Neural. 202, 57-66 https://doi.org/10.1016/j.expneurol.2006.05.011
  14. Dock, W. 1994. The evil sequlas of complete bed rest. J.A.M.A. 125, 1083-1085
  15. Dupont-Versteegden, E. E., J. D. Houle', C. M. Gurley, and C. A. Peterson. 1998. Early changes in muscle fiber size and gene expression in response to spinal cord transection and exercise. Am. J. Physiol. Cell Physiol. 275, C1124-C1133
  16. Dupont-Versteegden, E. E., R. J. L. Murphy, J. D. Houle', C. M. Gurley, and C. A. Peterson. 1999. Activated satellite cells fail to restore myonuclear number in spinal cord transected and exercised rats. Am. J. Physiol. Cell Physiol. 277, C589-C597
  17. Dupont-Versteegden, E. E., R. J. Murphy, J. D. Houle, C. M. Gurley, and C. A. Peterson. 2000. Mechanisms leading to restoration of muscle size with exercise and transplantation after spinal cord injury. Am. J. Physiol. Cell Physiol. 279, C1677 -C1684
  18. Elizabeth, J., G. Menon, S. Nair, and V. V. Radhakrishnan. 2001. Mixed tumour of schwannoma and meningioma in a patient with neurofibromatosis-2: a case report. Neurol. India 49, 398-400
  19. Engesser-Cesar, C., A. J. Anderson, D. M. Basso, V. R. Edgerton, and C. W. Cotman. 2005. Voluntary wheel running improves recovery from a moderate spinal cord injury. J. Neurotrauma 22, 157-171 https://doi.org/10.1089/neu.2005.22.157
  20. FIorini, J. R., D. Z. Ewton, and S. A. Coolican. 1996. Growth hormone and the insulin-like growth factor system in myogenesis. Endocr. Rev. 17, 481-517
  21. Genset J. C., C. A Tovar, F. P. Hamers, R. J. Deibert, M. S. Beattie, and J. C. Bresnahan. 2006. Behavioral and histological characterization of unilateral cervical spinal cord contusion injury in rats. J. Neurotrauma 23, 6-54
  22. Giangregorio, L. and N. Mccartney. 2006. Bone loss and muscle atrophy in spinal cord injury: Epidemiology, fracture prediction, and rehabilitation strategies. J. spinal cord Med. 29, 489-500
  23. Houle', J. D., K. Morris, R. D. Skinner, E. Garcia-Rilt and C. A. Peterson. 1999. Effects of fetal spinal cord tissue transplants and cycling exercise on the soleus muscle in spinalized rats. Muscle Nerve 22, 846-856 https://doi.org/10.1002/(SICI)1097-4598(199907)22:7<846::AID-MUS6>3.0.CO;2-I
  24. Jeong, I. G. and J. H. Yoon. 2006. Human Performance & Exercise Physiology, pp. 215-225, Daekyung Books, Seoul
  25. Kasper, C. E., T. P. White, and L. C. Maxwell. 1982. Adaptation of rat skeletal muscle to hypokinesia. The physiologist 25, 260
  26. Kasper, C. E., T. P. White, and L. C. Maxwell. 1990. Running during recovery from hindlimb suspension induces transient muscle injury. J. Appl. Physiol. 68, 533-539
  27. Kopple, J. D., H. Wang, M. Fournier, T.,Storer, S. M. Zhang, H. Y. Song, and M. Lewis. 2006. Transcriptional levels of growth factors in skeletal muscle of maintenance hemodialysis patients. J. Ren. Nutr. 16, 212-215 https://doi.org/10.1053/j.jrn.2006.04.013
  28. Lefaucheur, J. P. and A. Sebille. 1995. Muscle regeneration following injury can be modified in vivo by immune neutralization of basic fibroblast growth factor, transforming growth factor b1 or insulin-like growth factor I. J. Neuroimmunol. 57, 85-91 https://doi.org/10.1016/0165-5728(94)00166-L
  29. Lefaucheur, J. P., B. Gjata, H. Lafont, and A. Sebille. 1996. Angiogenic and inflammatory responses following skeletal muscle injury are altered by immune neutralization of endogenous basic fibroblast growth factor, insulin-like growth factor-1 and transforming growth factor-bl. J. Neuroimmunol. 70, 37-44 https://doi.org/10.1016/S0165-5728(96)00099-9
  30. McCall, G. E., D. L. Allen, J. K. Linderman, R. E. Grindeland, R. R. Roy, V. R. Mukku, and V. R. Edgerton. 1998. Maintenance of myonuclear domain size in rat soleus after overload and growth hormone/IGF-1 treatment. J. Appl. Physiol. 84, 1407-1412
  31. Murphy, R. J. L., E. E. Dupont-Versteegden, C. A. Peterson, and J. D. Houle. 1999. Two experimental strategies to restore muscle mass in adult rats following spinal cord injury. Neurorehab Neural Repair 13, 125-134 https://doi.org/10.1177/154596839901300205
  32. Musacchia, X. J., J. M. Steffen, and K. R. Deavers. 1981. Suspension restraint: induced hypokinesia and antiorthostasis as a simulation of weightlessness. The Physiologist 246 (Suppl)
  33. Oh, M. J., T. B. Seo, K. B. Kwon, S. J. Yoon, D. J. Elzi, B. G. Kim, and U. Namgung. 2009. Axonal outgrowth and Erk1/2 activation by training after spinal cord injury in rats. J. Neurotrauma May 26. [Epub ahead of print]
  34. Phelan, J. N. and W. J. Gonyea. 1997. Effect of radiation on satellite cell activity and protein expression in overloaded mammalian skeletal muscle. Anat. Rec. 247, 179-188 https://doi.org/10.1002/(SICI)1097-0185(199702)247:2<179::AID-AR4>3.0.CO;2-T
  35. Roberts, D. and D. J. Smith. 1989. Biochemical aspects of peripheral muscle fatigue: A Review. Sports Med. 7, 125-138 https://doi.org/10.2165/00007256-198907020-00004
  36. Rosenblatt, J. D., D. Yong, and D. J. Parry. 1994. Satellite cell activity is required for hypertrophy of overloaded adult rat muscle. Muscle Nerve 17, 608-613 https://doi.org/10.1002/mus.880170607
  37. Roy, R. R., and L. J. Acosta. 1986. Fiber type and fiber size changes in selected thigh muscles six months after low thoracic spinal cord transection in adult cats: exercise effects. Exp. Neurol. 92, 675-685 https://doi.org/10.1016/0014-4886(86)90308-0
  38. Roy, R. R., S. R. Monke, D. L. Allen, and V. R. Edgerton. 1999. Modulation of myonuclear number in functionally overloaded and exercised rat plantaris fibers. J. Appl. Physiol. 87, 634-642
  39. Roy, R. R., R. J. Talmadge, J. A. Hodgson, H. Zhong, K. M. Baldwin, and V. R. Edgerton. 1998. Training effects on soleus of cats spinal cord transected (T12-13) as adults. Muscle Nerve 21, 63-71 https://doi.org/10.1002/(SICI)1097-4598(199801)21:1<63::AID-MUS9>3.0.CO;2-E
  40. Schucht, P., O. Raineteau, M. E. Schwab, and K. Fouad. 2002. Anatomical correlates of locomotor recovery following dorsal and ventral lesions of the rat spinal cord. Exp. Neurol. 176, 143-153 https://doi.org/10.1006/exnr.2002.7909
  41. Spungen, A. M., R. H. Adkins, and C. A. Stewart. 2003. Factors influencing body composition in persons with spinal cord injury: a cross-sectional study. J. Appl. Physiol. 2398-2407
  42. Thota, A., S. Carlson, and R. Jung. 2001. Recovery of locomotor function after treadmill training of incomplete spinal cord injured rats. Biomed. Sci. Instrum. 37, 63-67
  43. Wilmet, E., A. A. Ismait, A. Heilporn, D. Welraeds, and P. Bergmann. 1995. Longitudinal study of the bone mineral content and of soft tissue composition after spinal cord section. Paraplegia 33, 674-677 https://doi.org/10.1038/sc.1995.141
  44. Winchester, P. K. and W. J. Gonyea. 1992. A quantitative study of satellite cells and myonuclei in stretched avian slow tonic muscle. Anat. Rec. 232, 369-377 https://doi.org/10.1002/ar.1092320306