생명과학회지 (Journal of Life Science)

  • 제19권12호
  • /
  • Pages.1841-1846
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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갱년기 유도 흰쥐에서 감태 추출물이 골 대사 지표물질의 변화에 미치는 영향

The Effect of Ecklonia cava Extracts on Bone Turnover Markers in Ovariectomized Rats

  • 강성림 (신라대학교 식품영양학과) ;
  • 김미향 (신라대학교 식품영양학과)
  • 발행 : 2009.12.30
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초록

갱년기의 여성에는 여러 폐경 증후들이 나타나는데, 특히 에스트로겐의 감소로 인한 골 손실의 증가 등으로 골다공증질환의 발병율이 높아지게 된다. 이러한 갱년기 증후군을 치료하기 위해 호르몬 대체 요법으로 치료를 하고 있으나 유방암과 자궁암 등의 부작용에 대한 논쟁의 여지가 있어 갱년기 증후군을 치료하는데 양면성을 가진다. 그러므로 본 연구에서는 쉽게 섭취 할 수 있으며, 부작용이 없는 해조류인 감태 추출물을 이용하여 in vivo 실험을 통해 갱년기 장애 시 나타날 수 있는 골 손실에 미치는 영향을 검토하였다. 난소 절제술을 시행한 군들에서 전체적으로 체중이 증가하였으나, 난소절제 후 감태 추출물을 투여에 의한 체중 감소는 나타나지 않아 감태 추출물이 체중 감소에는 크게 영향을 미치지 않은 것으로 나타났다. 난소절제 시(OVX-CON) 에스트로겐 결핍으로 bone turnover가 증가되어 비 난소절제(SHAM)군에 비해 혈장 중의 ALP의 활성이 증가되었으나, 난소 절제 후 감태 추출물을 투여한 군에서 그 활성이 유의적으로 감소하는 경향이 나타났다. 이것은 난소 절제 후 에스트로겐의 분비가 감소되는데 반해 감태 추출물 투여가 에스트로겐 대체 작용을 함으로써 난소절제로 인한 골 손실 정도를 완화시켜 준 것으로 추측되어진다. 골 용해 지표인 혈액 중의 CTx는 난소절제군이 비 절제군에 비해 높은 수준을 보여 난소 절제군에 있어 골 용해가 증가되었음을 나타내었다. 반면 감태 추출물의 투여로 인해 CTx 함량이 감소하였으며, EC-200군의 경우 SHAM과 비슷한 수준으로 나타났다. 이는 난소절제로 인해 bone turnover가 증가된 상태에서 감태 추출물의 투여로 bone turnover가 감소하여 골 흡수 저해 효과를 타나낸 것으로 추정된다. 또한 결합조직 중의 collagen 함량은 난소절제로 인하여 감소하였으나, 감태 추출물의 투여에 의해 점진적으로 회복되는 경향을 나타내었다. 이상의 결과로부터 estrogen 부족으로 인한 골 손실의 예방에 감태가 유익한 효과를 가지며, 이를 활용한 기능성식품 개발도 가능할 것으로 기대되어 진다.

Menopause is often associated with the incidence of several chronic diseases including osteoporosis, cardiovascular disease, and obesity. Hormone replacement therapy (HRT) is an effective regimen that has been found to prevent these diseases in postmenopausal women. However, HRT is accompanied by an increased risk of unfavorable outcomes. Therefore, this study was conducted to evaluate the effects of Ecklonia cava, a kind of seaweed, extract on bone turnover markers in symptomatic menopausal women. For this study, the following four groups of 9-week-old Sprague-Dawley rats were evaluated over 6 weeks: normal rats (SHAM), ovariectomized rats (OVX-CON) and ovariectomized rats that were treated with Ecklonia cava extracts. The optimum extraction temperature and solvent of Ecklonia cava were found to be $80^{\circ}C$ and 80% ethanol. We measured the osteocalcin and CTx content, enzyme ALP activity in serum and collagen content in the cartilage, bone, skin and lungs. We found that the levels of indicators of bone metabolism such as ALP, osteocalcin and CTx were lower in rats in the Ecklonia cava extract group than the OVX-CON group. In addition, the collagen contents in the bone, cartilage, skin and lungs decreased in response to ovariectomy, but the levels of collagen were greater in the bone of rats that were treated with Ecklonia cava extract than in the bone of rats in the OVX-CON group. According to these results, we were able to know the effects of Ecklonia cava extract on bone aging in ovariectomized rats. Consequently, we expect Ecklonia cava extract to have an effect on bone aging in postmenopausal women.

키워드

참고문헌

  1. Ahn, M. J., K. D. Yoon, S. Y. Min, J. S. Lee, J. H. Kim, T. G. Kim, S. H. Kim, N. G. Huh, and H. J. Kim. 2004. Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. BioI. Pharm. Bull. 27, 544-547 https://doi.org/10.1248/bpb.27.544
  2. Bonde, M., P. Carnero, C. Fledelius, P. Qvist, P. D. Delmas, and C. Christiansen. 1997. Measurement of bone degradation products in serum using antibodies reactive with an isomerized from of an 8 amino acid sequence of the C-telopeptide of type I collagen. J. Bone Miner. Rse. 12, 1028-1034 https://doi.org/10.1359/jbmr.1997.12.7.1028
  3. Brown, J. P., P. D. Delmas, L. Malaval, C. Edouard, M. C. Chapuy, and P. J. Meunier. 1984. Serum bone CIa-protein: a specific marker for bone formation in postmenopausal osteoporosis. Lancet. 19, 1091-1093
  4. Cho, K. J., Y. S. Lee, and B. H. Ryu. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull. Kor. Fish Soc. 23, 345-352
  5. Clark, A. P. and J. A. Schuttinga. 1992. Targeted estrogen/progesterone replacement therapy for osteoporosis: calculation of health care cost savings. Osteaporos. Int. 2, 195-200 https://doi.org/10.1007/BF01623926
  6. Dempster, D. W., R Birchman, and R Xu. 1995. Temporal changes in cancellous bone structure of rats immediately after ovariectomy. Bone 16, 157-161 https://doi.org/10.1016/S8756-3282(94)00024-7
  7. Einhorn, T. A. 1996. The bone organ system. Form and function. pp. 3-222, In Marcus, R, D. Feldmen, J. Kelsey (eds.), Osteoporosis. London, Academic press Inc
  8. Carnero, P., E. Hausherr, M. C. Chapuy, C. Marcelli, H. Carndjean, C. Muller, C. Cormier, G. Breart, P. J. Meunier, and P. D. Delmas. 1996. Markers of bone resorption predict hip fracture in elderly woman: the EPIDOS Prospective Study. J. Bone Miner. Res. 11, 1531-1538 https://doi.org/10.1002/jbmr.5650111021
  9. Croen, J. J., J. MenczeC and S. Shapiro. 1968. Chronic destructive periodontal disease in patients with presenile osteoporosis. J. Periodontal. 39, 19-23 https://doi.org/10.1016/0026-0495(86)90202-7
  10. Han, I. K., K. O. Park, H. Kim, and N. H. Cho. 1998. Climacteric symptoms and perception in middle-aged Korean women. 4, 3-15
  11. Hong, J. H., B. K. Kim, B. S. Son, H. Y. Chee, B. H. Lee, H. C. Shin, K. B. Lee, and K. S. Song. 2006. Antihypertensive effect of Ecklonia cava extract. Kor. Soc. Pharm. 37, 200-205
  12. SimI J. M. 2003. Studies on the nutritional evaluation of Ecklonia cava kjellman and Ecklonia Residues and their dietary effects on immunocompetence and productivity in chicken. Ph.D. Program in the Department of Animal Science, Graduate School of Konkuk University
  13. Jilka, R. L. 1998. Cytokines, bone remodeling, and estrogen deficiency: a 1998 update. Bone 23, 75-81 https://doi.org/10.1016/S8756-3282(98)00077-5
  14. Kang, B. M., M. R. Kim, B. K. Yoon, B. S. Lee, H. W. Chung, H. Choi, H. M. Park, and J. G. Kim. 2007. Effect of hormone replacement therapy on bone mineral density in Korean postmenopausal women over the age of 60. 13, 131-138
  15. Kassem, K., K. Brixen, and L. Mosekilde. 1998. Effect of growth hormone treatment on serum levels of insulin-like growth factors and IGF binding protein 1-4 in postmenopausal woman. Clinical Endocrinology 49, 747-756 https://doi.org/10.1046/j.1365-2265.1998.00606.x
  16. Khandwala, H. M., S. Mumm, and M. P. Whyte. 2006. Low serum alkaline phosphatase activity and pathologic fracture: case report and brief review of hypophosphatasia diagnosed in adulthood. Endocr. Pract. 12, 676-681 https://doi.org/10.4158/EP.12.6.676
  17. Kimble, R. B., A. B. Matayoshi, and J. L. Vannice. 1995. Simulataneous block of interleukin-1 and tumor necrosis factor is required to completely prevent bone loss in the early postovariectomy period. Endocrinology 136, 3054-3061 https://doi.org/10.1210/en.136.7.3054
  18. Kimble, R. B., J. L. Vannice, and D. C. Bloedowl. 1994. Interleukin-1 receptor antagonist decreases bone loss and bone resorption in ovariectomized rats. J. Clin. Invest. 93, 1959-1967 https://doi.org/10.1172/JCI117187
  19. Kim, S, A., J. Kim, M. K. Woo, C. S. Kwak, and M. S. Lee. 2005. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J. Korean Soc. Food Sci. Nutr. 34, 451-459 https://doi.org/10.3746/jkfn.2005.34.4.451
  20. Kwon, M. J. and T. J. Nam. 2006. Effects of Mesangi (Capsosiphon fulvecens) power on lipid metabolism in high cholesterol fed rats. J. Korean Soc. Food Sci. Nutr. 35, 530-535 https://doi.org/10.3746/jkfn.2006.35.5.530
  21. Lee, H. S., M. S. Choi, Y. K. Lee, S. H. Park, and Y. J. Kim. 1996. A study on the development of high-fiber supplements for the diabetic patients-Effect of seaweed supplement on the lipid and glucose metabolism in streptozotocin induced diabetic rats. Korean J. Nutr. 29, 296-306
  22. Lee, S. H., K. N. Kim, S. H. Cha, G. N. Ahn, and Y. J. Jeon. 2006. Comparison of antioxidant activities of enzymatic and methanolic extracts from Ecklonia cava stem and leave. J. Korean Soc. Food Sci. Nutr. 35, 1139-1145 https://doi.org/10.3746/jkfn.2006.35.9.1139
  23. Lee, Y. A. and M. Kim. 2008. Effects of sea tangle extract on formation of collagen and collagen cross-link in ovariectomized rats. J. Life Sci. 18, 1578-1583
  24. Lindsay, R. 1992. Osteoprosis. pp. 8-33, A guide to diagnosis, prevention, and treatment. Paven press. New York
  25. Osmanagaoglu, M. A., S. Osmanagaoglu, T. Osmanagaoglu, B. Okumus, and H. Ozkaya. 2005. Effect of different preparations of hormone therapy on lipid and glucose metabolism, coagulation factors and bone mineral density in overweightand obese postmenopausal woman. Fertility and Sterility 84, 384-393 https://doi.org/10.1016/j.fertnstert.2005.01.131
  26. Myung, C. S., H. C. Shin, H. Y. Bao, S. J. Yeo, B. H. Lee, and J. S. Kang. 2005. Improvement of memory by dieckol and phlorofucofuroeckol in ethanol-treated mice: possible involvement of the inhibition of acetylcholinesterase. Arch. Pharm. Res. 28, 691-698 https://doi.org/10.1007/BF02969360
  27. Newton, K. M., S. D. Reed, L. Grothaus, J. Guiltinan, E. Ludman, and A. Z. Lacroix. 2005. The Herbal Alternatives for Menopause Study (HALT): background and study design. Maturitas. 52, 134-146 https://doi.org/10.1016/j.maturitas.2005.01.007
  28. Osmers, R., M. Friede, E. Liske, J. Schnitker, J. Feudenstein, and H. H. Henneicke-von Zepelin. 2005. Efficacy and safety of isopropanolic black cohosh extract for climacteric symptoms. Obstet Gynecol. 105, 1074-1083 https://doi.org/10.1097/01.AOG.0000158865.98070.89
  29. Park, H. M., T. C. Kim, T. C. Kim, K. H. Kang, S. J. Yoon, and M. Hur. 2000. The predictive value of changes of bone markers for changes of bone mineral density in postmenopausal hormone replacement therapy with or without active vitamin D. Kor. Soc. Obstetrics Gynecology 43, 268-274
  30. Pi Y. Z., X. P. Wu, S. P. Liu, X. H Luo, X. Z. Cao, H. Xie, and E. Y. Liao. 2006. Age-related changes in bone biochemical markers and their relationship with bone mineral density in normal Chinese woman. J. Bone Miner. Res. 24, 380-385 https://doi.org/10.1007/s00774-006-0703-2
  31. Price, P. A., J. G. Parthermone, and L.. J. Doftos. 1980. New biochemical marker for bone metabolism. J. Clin. Invest. 66, 878-883 https://doi.org/10.1172/JCI109954
  32. Riis, B. J. 1993. Biochemical markers of bone turnover. II: Diagnosis, prophylaxis, and treatment of osteoporosis. Am. J. Med. 95, 17S-21S https://doi.org/10.1016/0002-9343(93)90376-Z
  33. Schiele, F., J. Henny, J. Hitz, C. Petitclerc, R Gueguen and G. Siest. 1983. Total bone and liver alkaline phosphatases in plasma: biological variations and reference limits. Clin. Chem. 29, 634-641 https://doi.org/10.1016/0009-8981(88)90014-9
  34. Shin, H. C., H. J. Hwang, K. J. Kang, and B. H. Lee. 2006. An antioxidative and antiinflammatory agent for potential treatment of osteoarthritis from Ecklonia. cava. Arch. Pharm. Res. 29, 165-171 https://doi.org/10.1007/BF02974279
  35. Solt, D. B. 1991. The pathogenesis, oral manifestations, and implications for dentistry of metabolic bone disease. Curro Opin. Dent. 1, 783-791
  36. Stampfer, M. J. and G. A Colditz. 1991. Estrogen replacement therapy and coronary heart disease: a quantitative assessment of the epidemiologic evidence. Prevo Med. 20, 47-63 https://doi.org/10.1016/0091-7435(91)90006-P
  37. The Committee of Menopause. 2008. Relationship between osteoporosis and cardiovascular disease in postmenopausal women. 14, 238-245
  38. Turner, R. T., G. K. Wakley, and K. S. Hannon. 1974. Tamoxifen prevents the skeletal effects of ovarian hormone deficiency in rats. J. Bone Mineral Res. 2, 449-459 https://doi.org/10.1002/jbmr.5650020513
  39. Woessner, J. F. 1961. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch. Biochem. Biophys. 93, 440-447
  40. Yamazaki, I. and H. Yamaguchi. 1989. Characteristics of an ovariectomized osteopenic rat model. J. Bone Min. Res. 4, 13-22 https://doi.org/10.1002/jbmr.5650040104

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